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Antifungal Action of Arabidopsis thaliana TCP21 via Induction of Oxidative Stress and Apoptosis

The realm of antimicrobial proteins in plants is extensive but remains relatively uncharted. Understanding the mechanisms underlying the action of plant antifungal proteins (AFPs) holds promise for antifungal strategies. This study aimed to bridge this knowledge gap by comprehensively screening Arab...

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Autores principales: Park, Seong-Cheol, Yoon, A-Mi, Kim, Young-Min, Lee, Min-Young, Lee, Jung Ro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10525234/
https://www.ncbi.nlm.nih.gov/pubmed/37760070
http://dx.doi.org/10.3390/antiox12091767
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author Park, Seong-Cheol
Yoon, A-Mi
Kim, Young-Min
Lee, Min-Young
Lee, Jung Ro
author_facet Park, Seong-Cheol
Yoon, A-Mi
Kim, Young-Min
Lee, Min-Young
Lee, Jung Ro
author_sort Park, Seong-Cheol
collection PubMed
description The realm of antimicrobial proteins in plants is extensive but remains relatively uncharted. Understanding the mechanisms underlying the action of plant antifungal proteins (AFPs) holds promise for antifungal strategies. This study aimed to bridge this knowledge gap by comprehensively screening Arabidopsis thaliana species to identify novel AFPs. Using MALDI-TOF analysis, we identified a member of the TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR1 (TCP) family of transcription factors as a novel AFP, A. thaliana TCP21 (AtTCP21; accession number NP_196450). Bacterially purified recombinant AtTCP21 inhibited the growth of various pathogenic fungal cells. AtTCP21 was more potent than melittin, a well-known AFP, in combating Colletotrichum gloeosporioides. Growth inhibition assays against various fungal pathogens and yeasts confirmed the pH-dependent antimicrobial activity of AtTCP21. Without inducing any membrane alterations, AtTCP21 penetrates the fungal cell wall and membrane, where it instigates a repressive milieu for fungal cell growth by generating intracellular reactive oxygen species and mitochondrial superoxides; resulting in morphological changes and apoptosis. Our findings demonstrate the redox-regulating effects of AtTCP21 and point to its potential as an antimicrobial agent.
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spelling pubmed-105252342023-09-28 Antifungal Action of Arabidopsis thaliana TCP21 via Induction of Oxidative Stress and Apoptosis Park, Seong-Cheol Yoon, A-Mi Kim, Young-Min Lee, Min-Young Lee, Jung Ro Antioxidants (Basel) Article The realm of antimicrobial proteins in plants is extensive but remains relatively uncharted. Understanding the mechanisms underlying the action of plant antifungal proteins (AFPs) holds promise for antifungal strategies. This study aimed to bridge this knowledge gap by comprehensively screening Arabidopsis thaliana species to identify novel AFPs. Using MALDI-TOF analysis, we identified a member of the TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR1 (TCP) family of transcription factors as a novel AFP, A. thaliana TCP21 (AtTCP21; accession number NP_196450). Bacterially purified recombinant AtTCP21 inhibited the growth of various pathogenic fungal cells. AtTCP21 was more potent than melittin, a well-known AFP, in combating Colletotrichum gloeosporioides. Growth inhibition assays against various fungal pathogens and yeasts confirmed the pH-dependent antimicrobial activity of AtTCP21. Without inducing any membrane alterations, AtTCP21 penetrates the fungal cell wall and membrane, where it instigates a repressive milieu for fungal cell growth by generating intracellular reactive oxygen species and mitochondrial superoxides; resulting in morphological changes and apoptosis. Our findings demonstrate the redox-regulating effects of AtTCP21 and point to its potential as an antimicrobial agent. MDPI 2023-09-15 /pmc/articles/PMC10525234/ /pubmed/37760070 http://dx.doi.org/10.3390/antiox12091767 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Seong-Cheol
Yoon, A-Mi
Kim, Young-Min
Lee, Min-Young
Lee, Jung Ro
Antifungal Action of Arabidopsis thaliana TCP21 via Induction of Oxidative Stress and Apoptosis
title Antifungal Action of Arabidopsis thaliana TCP21 via Induction of Oxidative Stress and Apoptosis
title_full Antifungal Action of Arabidopsis thaliana TCP21 via Induction of Oxidative Stress and Apoptosis
title_fullStr Antifungal Action of Arabidopsis thaliana TCP21 via Induction of Oxidative Stress and Apoptosis
title_full_unstemmed Antifungal Action of Arabidopsis thaliana TCP21 via Induction of Oxidative Stress and Apoptosis
title_short Antifungal Action of Arabidopsis thaliana TCP21 via Induction of Oxidative Stress and Apoptosis
title_sort antifungal action of arabidopsis thaliana tcp21 via induction of oxidative stress and apoptosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10525234/
https://www.ncbi.nlm.nih.gov/pubmed/37760070
http://dx.doi.org/10.3390/antiox12091767
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