Cargando…

Comparison of Mid-Infrared Handheld and Benchtop Spectrometers to Detect Staphylococcus epidermidis in Bone Grafts

Bone analyses using mid-infrared spectroscopy are gaining popularity, especially with handheld spectrometers that enable on-site testing as long as the data quality meets standards. In order to diagnose Staphylococcus epidermidis in human bone grafts, this study was carried out to compare the effect...

Descripción completa

Detalles Bibliográficos
Autores principales: Lindtner, Richard, Wurm, Alexander, Kugel, Katrin, Kühn, Julia, Putzer, David, Arora, Rohit, Coraça-Huber, Débora Cristina, Zelger, Philipp, Schirmer, Michael, Badzoka, Jovan, Kappacher, Christoph, Huck, Christian Wolfgang, Pallua, Johannes Dominikus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10525239/
https://www.ncbi.nlm.nih.gov/pubmed/37760120
http://dx.doi.org/10.3390/bioengineering10091018
_version_ 1785110736080470016
author Lindtner, Richard
Wurm, Alexander
Kugel, Katrin
Kühn, Julia
Putzer, David
Arora, Rohit
Coraça-Huber, Débora Cristina
Zelger, Philipp
Schirmer, Michael
Badzoka, Jovan
Kappacher, Christoph
Huck, Christian Wolfgang
Pallua, Johannes Dominikus
author_facet Lindtner, Richard
Wurm, Alexander
Kugel, Katrin
Kühn, Julia
Putzer, David
Arora, Rohit
Coraça-Huber, Débora Cristina
Zelger, Philipp
Schirmer, Michael
Badzoka, Jovan
Kappacher, Christoph
Huck, Christian Wolfgang
Pallua, Johannes Dominikus
author_sort Lindtner, Richard
collection PubMed
description Bone analyses using mid-infrared spectroscopy are gaining popularity, especially with handheld spectrometers that enable on-site testing as long as the data quality meets standards. In order to diagnose Staphylococcus epidermidis in human bone grafts, this study was carried out to compare the effectiveness of the Agilent 4300 Handheld Fourier-transform infrared with the Perkin Elmer Spectrum 100 attenuated-total-reflectance infrared spectroscopy benchtop instrument. The study analyzed 40 non-infected and 10 infected human bone samples with Staphylococcus epidermidis, collecting reflectance data between 650 cm(−1) and 4000 cm(−1), with a spectral resolution of 2 cm(−1) (Agilent 4300 Handheld) and 0.5 cm(−1) (Perkin Elmer Spectrum 100). The acquired spectral information was used for spectral and unsupervised classification, such as a principal component analysis. Both methods yielded significant results when using the recommended settings and data analysis strategies, detecting a loss in bone quality due to the infection. MIR spectroscopy provides a valuable diagnostic tool when there is a tissue shortage and time is of the essence. However, it is essential to conduct further research with larger sample sizes to verify its pros and cons thoroughly.
format Online
Article
Text
id pubmed-10525239
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-105252392023-09-28 Comparison of Mid-Infrared Handheld and Benchtop Spectrometers to Detect Staphylococcus epidermidis in Bone Grafts Lindtner, Richard Wurm, Alexander Kugel, Katrin Kühn, Julia Putzer, David Arora, Rohit Coraça-Huber, Débora Cristina Zelger, Philipp Schirmer, Michael Badzoka, Jovan Kappacher, Christoph Huck, Christian Wolfgang Pallua, Johannes Dominikus Bioengineering (Basel) Article Bone analyses using mid-infrared spectroscopy are gaining popularity, especially with handheld spectrometers that enable on-site testing as long as the data quality meets standards. In order to diagnose Staphylococcus epidermidis in human bone grafts, this study was carried out to compare the effectiveness of the Agilent 4300 Handheld Fourier-transform infrared with the Perkin Elmer Spectrum 100 attenuated-total-reflectance infrared spectroscopy benchtop instrument. The study analyzed 40 non-infected and 10 infected human bone samples with Staphylococcus epidermidis, collecting reflectance data between 650 cm(−1) and 4000 cm(−1), with a spectral resolution of 2 cm(−1) (Agilent 4300 Handheld) and 0.5 cm(−1) (Perkin Elmer Spectrum 100). The acquired spectral information was used for spectral and unsupervised classification, such as a principal component analysis. Both methods yielded significant results when using the recommended settings and data analysis strategies, detecting a loss in bone quality due to the infection. MIR spectroscopy provides a valuable diagnostic tool when there is a tissue shortage and time is of the essence. However, it is essential to conduct further research with larger sample sizes to verify its pros and cons thoroughly. MDPI 2023-08-29 /pmc/articles/PMC10525239/ /pubmed/37760120 http://dx.doi.org/10.3390/bioengineering10091018 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lindtner, Richard
Wurm, Alexander
Kugel, Katrin
Kühn, Julia
Putzer, David
Arora, Rohit
Coraça-Huber, Débora Cristina
Zelger, Philipp
Schirmer, Michael
Badzoka, Jovan
Kappacher, Christoph
Huck, Christian Wolfgang
Pallua, Johannes Dominikus
Comparison of Mid-Infrared Handheld and Benchtop Spectrometers to Detect Staphylococcus epidermidis in Bone Grafts
title Comparison of Mid-Infrared Handheld and Benchtop Spectrometers to Detect Staphylococcus epidermidis in Bone Grafts
title_full Comparison of Mid-Infrared Handheld and Benchtop Spectrometers to Detect Staphylococcus epidermidis in Bone Grafts
title_fullStr Comparison of Mid-Infrared Handheld and Benchtop Spectrometers to Detect Staphylococcus epidermidis in Bone Grafts
title_full_unstemmed Comparison of Mid-Infrared Handheld and Benchtop Spectrometers to Detect Staphylococcus epidermidis in Bone Grafts
title_short Comparison of Mid-Infrared Handheld and Benchtop Spectrometers to Detect Staphylococcus epidermidis in Bone Grafts
title_sort comparison of mid-infrared handheld and benchtop spectrometers to detect staphylococcus epidermidis in bone grafts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10525239/
https://www.ncbi.nlm.nih.gov/pubmed/37760120
http://dx.doi.org/10.3390/bioengineering10091018
work_keys_str_mv AT lindtnerrichard comparisonofmidinfraredhandheldandbenchtopspectrometerstodetectstaphylococcusepidermidisinbonegrafts
AT wurmalexander comparisonofmidinfraredhandheldandbenchtopspectrometerstodetectstaphylococcusepidermidisinbonegrafts
AT kugelkatrin comparisonofmidinfraredhandheldandbenchtopspectrometerstodetectstaphylococcusepidermidisinbonegrafts
AT kuhnjulia comparisonofmidinfraredhandheldandbenchtopspectrometerstodetectstaphylococcusepidermidisinbonegrafts
AT putzerdavid comparisonofmidinfraredhandheldandbenchtopspectrometerstodetectstaphylococcusepidermidisinbonegrafts
AT arorarohit comparisonofmidinfraredhandheldandbenchtopspectrometerstodetectstaphylococcusepidermidisinbonegrafts
AT coracahuberdeboracristina comparisonofmidinfraredhandheldandbenchtopspectrometerstodetectstaphylococcusepidermidisinbonegrafts
AT zelgerphilipp comparisonofmidinfraredhandheldandbenchtopspectrometerstodetectstaphylococcusepidermidisinbonegrafts
AT schirmermichael comparisonofmidinfraredhandheldandbenchtopspectrometerstodetectstaphylococcusepidermidisinbonegrafts
AT badzokajovan comparisonofmidinfraredhandheldandbenchtopspectrometerstodetectstaphylococcusepidermidisinbonegrafts
AT kappacherchristoph comparisonofmidinfraredhandheldandbenchtopspectrometerstodetectstaphylococcusepidermidisinbonegrafts
AT huckchristianwolfgang comparisonofmidinfraredhandheldandbenchtopspectrometerstodetectstaphylococcusepidermidisinbonegrafts
AT palluajohannesdominikus comparisonofmidinfraredhandheldandbenchtopspectrometerstodetectstaphylococcusepidermidisinbonegrafts