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Comparison of Mid-Infrared Handheld and Benchtop Spectrometers to Detect Staphylococcus epidermidis in Bone Grafts
Bone analyses using mid-infrared spectroscopy are gaining popularity, especially with handheld spectrometers that enable on-site testing as long as the data quality meets standards. In order to diagnose Staphylococcus epidermidis in human bone grafts, this study was carried out to compare the effect...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10525239/ https://www.ncbi.nlm.nih.gov/pubmed/37760120 http://dx.doi.org/10.3390/bioengineering10091018 |
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author | Lindtner, Richard Wurm, Alexander Kugel, Katrin Kühn, Julia Putzer, David Arora, Rohit Coraça-Huber, Débora Cristina Zelger, Philipp Schirmer, Michael Badzoka, Jovan Kappacher, Christoph Huck, Christian Wolfgang Pallua, Johannes Dominikus |
author_facet | Lindtner, Richard Wurm, Alexander Kugel, Katrin Kühn, Julia Putzer, David Arora, Rohit Coraça-Huber, Débora Cristina Zelger, Philipp Schirmer, Michael Badzoka, Jovan Kappacher, Christoph Huck, Christian Wolfgang Pallua, Johannes Dominikus |
author_sort | Lindtner, Richard |
collection | PubMed |
description | Bone analyses using mid-infrared spectroscopy are gaining popularity, especially with handheld spectrometers that enable on-site testing as long as the data quality meets standards. In order to diagnose Staphylococcus epidermidis in human bone grafts, this study was carried out to compare the effectiveness of the Agilent 4300 Handheld Fourier-transform infrared with the Perkin Elmer Spectrum 100 attenuated-total-reflectance infrared spectroscopy benchtop instrument. The study analyzed 40 non-infected and 10 infected human bone samples with Staphylococcus epidermidis, collecting reflectance data between 650 cm(−1) and 4000 cm(−1), with a spectral resolution of 2 cm(−1) (Agilent 4300 Handheld) and 0.5 cm(−1) (Perkin Elmer Spectrum 100). The acquired spectral information was used for spectral and unsupervised classification, such as a principal component analysis. Both methods yielded significant results when using the recommended settings and data analysis strategies, detecting a loss in bone quality due to the infection. MIR spectroscopy provides a valuable diagnostic tool when there is a tissue shortage and time is of the essence. However, it is essential to conduct further research with larger sample sizes to verify its pros and cons thoroughly. |
format | Online Article Text |
id | pubmed-10525239 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105252392023-09-28 Comparison of Mid-Infrared Handheld and Benchtop Spectrometers to Detect Staphylococcus epidermidis in Bone Grafts Lindtner, Richard Wurm, Alexander Kugel, Katrin Kühn, Julia Putzer, David Arora, Rohit Coraça-Huber, Débora Cristina Zelger, Philipp Schirmer, Michael Badzoka, Jovan Kappacher, Christoph Huck, Christian Wolfgang Pallua, Johannes Dominikus Bioengineering (Basel) Article Bone analyses using mid-infrared spectroscopy are gaining popularity, especially with handheld spectrometers that enable on-site testing as long as the data quality meets standards. In order to diagnose Staphylococcus epidermidis in human bone grafts, this study was carried out to compare the effectiveness of the Agilent 4300 Handheld Fourier-transform infrared with the Perkin Elmer Spectrum 100 attenuated-total-reflectance infrared spectroscopy benchtop instrument. The study analyzed 40 non-infected and 10 infected human bone samples with Staphylococcus epidermidis, collecting reflectance data between 650 cm(−1) and 4000 cm(−1), with a spectral resolution of 2 cm(−1) (Agilent 4300 Handheld) and 0.5 cm(−1) (Perkin Elmer Spectrum 100). The acquired spectral information was used for spectral and unsupervised classification, such as a principal component analysis. Both methods yielded significant results when using the recommended settings and data analysis strategies, detecting a loss in bone quality due to the infection. MIR spectroscopy provides a valuable diagnostic tool when there is a tissue shortage and time is of the essence. However, it is essential to conduct further research with larger sample sizes to verify its pros and cons thoroughly. MDPI 2023-08-29 /pmc/articles/PMC10525239/ /pubmed/37760120 http://dx.doi.org/10.3390/bioengineering10091018 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lindtner, Richard Wurm, Alexander Kugel, Katrin Kühn, Julia Putzer, David Arora, Rohit Coraça-Huber, Débora Cristina Zelger, Philipp Schirmer, Michael Badzoka, Jovan Kappacher, Christoph Huck, Christian Wolfgang Pallua, Johannes Dominikus Comparison of Mid-Infrared Handheld and Benchtop Spectrometers to Detect Staphylococcus epidermidis in Bone Grafts |
title | Comparison of Mid-Infrared Handheld and Benchtop Spectrometers to Detect Staphylococcus epidermidis in Bone Grafts |
title_full | Comparison of Mid-Infrared Handheld and Benchtop Spectrometers to Detect Staphylococcus epidermidis in Bone Grafts |
title_fullStr | Comparison of Mid-Infrared Handheld and Benchtop Spectrometers to Detect Staphylococcus epidermidis in Bone Grafts |
title_full_unstemmed | Comparison of Mid-Infrared Handheld and Benchtop Spectrometers to Detect Staphylococcus epidermidis in Bone Grafts |
title_short | Comparison of Mid-Infrared Handheld and Benchtop Spectrometers to Detect Staphylococcus epidermidis in Bone Grafts |
title_sort | comparison of mid-infrared handheld and benchtop spectrometers to detect staphylococcus epidermidis in bone grafts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10525239/ https://www.ncbi.nlm.nih.gov/pubmed/37760120 http://dx.doi.org/10.3390/bioengineering10091018 |
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