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Isolation and Identification of Endophytic Bacteria Bacillus sp. ME9 That Exhibits Biocontrol Activity against Xanthomonas phaseoli pv. manihotis

SIMPLE SUMMARY: Cassava is one of the most important crops worldwide. However, bacterial blight of cassava, as its most important disease, has caused substantial economic losses to the cassava industry. The main method of controlling cassava bacterial blight has been through chemical control, but th...

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Detalles Bibliográficos
Autores principales: Feng, Yating, Zhang, Yijie, Shah, Obaid Ullah, Luo, Kai, Chen, Yinhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10525512/
https://www.ncbi.nlm.nih.gov/pubmed/37759630
http://dx.doi.org/10.3390/biology12091231
Descripción
Sumario:SIMPLE SUMMARY: Cassava is one of the most important crops worldwide. However, bacterial blight of cassava, as its most important disease, has caused substantial economic losses to the cassava industry. The main method of controlling cassava bacterial blight has been through chemical control, but this has polluted the environment significantly. In this context, biological control using microorganisms not only has the capacity to inhibit the disease, but also is environmentally friendly, showing great potential. Therefore, there is an urgent need to study the endophytic biological control method of cassava bacterial blight. On this basis, in the current study, we conducted a series of in vivo and in vitro experiments to isolate, screen and identify a biocontrol Bacillus ME9 with an antagonistic effect against Xpm11, the pathogen that causes cassava bacterial blight. In this study, the results also demonstrated that B. ME9 has a strong colonization ability, and revealed that its genome consists of a variety of genes related to antibacterial lipopeptides, which may be directly related to its antibacterial ability. B. ME9 had a strong antagonistic affect against Xpm11, and showed a certain effect on a variety of pathogens, which is expected to be further developed in its application as a commercial bacteriological agent. ABSTRACT: In recent years, the bacterial blight of cassava has caused substantial economic losses to the Chinese cassava industry. Chemical control methods have become the primary approach to control this disease; however, their widespread usage and harmful residues have raised concerns about environmental pollution. In order to avoid this, it is urgent to seek a green ecological method to prevent and control it. Biological control through the utilization of microorganisms not only effectively inhibits the disease, but also gives consideration to environmental friendliness. Therefore, investigating an endophytic biological control method for cassava bacterial blight is of great importance. In this study, cassava leaf tissues were used as test specimens in order to isolate endophytic bacteria by using dilution and separation methods. Bacillus ME9, derived from cassava endophytic bacteria, exhibits good antagonism against a diverse range of pathogens, including Xpm11. Its genome consists of a series of genes encoding antibacterial lipopeptides, which may be directly related to its antibacterial capabilities. Furthermore, inoculation resulted in a substantial change in the diversity of the endophytic bacterial community, characterized by improved diversity, and displayed an obvious inhibition of pathogenic bacterial growth, demonstrating successful colonization within plants. The results laid a foundation and provided theoretical support for the development and utilization of cassava endophytic bacterial diversity and endogenous disease control strategies.