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Improved Cartilage Protection with Low Molecular Weight Hyaluronic Acid Hydrogel
Traumatic joint injuries are common, leading to progressive tissue degeneration and the development of osteoarthritis. The post-traumatic joint experiences a pro-inflammatory milieu, initiating a subtle but deteriorative process in cartilage tissue. To prevent or even reverse this process, our group...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10525634/ https://www.ncbi.nlm.nih.gov/pubmed/37760116 http://dx.doi.org/10.3390/bioengineering10091013 |
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author | Brackin, Riley B. McColgan, Gail E. Pucha, Saitheja A. Kowalski, Michael A. Drissi, Hicham Doan, Thanh N. Patel, Jay M. |
author_facet | Brackin, Riley B. McColgan, Gail E. Pucha, Saitheja A. Kowalski, Michael A. Drissi, Hicham Doan, Thanh N. Patel, Jay M. |
author_sort | Brackin, Riley B. |
collection | PubMed |
description | Traumatic joint injuries are common, leading to progressive tissue degeneration and the development of osteoarthritis. The post-traumatic joint experiences a pro-inflammatory milieu, initiating a subtle but deteriorative process in cartilage tissue. To prevent or even reverse this process, our group previously developed a tissue-penetrating methacrylated hyaluronic acid (MeHA) hydrogel system, crosslinked within cartilage to restore and/or protect the tissue. In the current study, we further optimized this approach by investigating the impact of biomaterial molecular weight (MW; 20, 75, 100 kDa) on its integration within and reinforcement of cartilage, as well as its ability to protect tissue degradation in a catabolic state. Indeed, the low MW MeHA integrated and reinforced cartilage tissue better than the high MW counterparts. Furthermore, in a 2 week IL-1β explant culture model, the 20 kDa MeHA demonstrated the most protection from biphasic mechanical loss, best retention of proteoglycans (Safranin O staining), and least aggrecan breakdown (NITEGE). Thus, the lower MW MeHA gels integrated better into the tissue and provided the greatest protection of the cartilage matrix. Future work will test this formulation in a preclinical model, with the goal of translating this therapeutic approach for cartilage preservation. |
format | Online Article Text |
id | pubmed-10525634 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105256342023-09-28 Improved Cartilage Protection with Low Molecular Weight Hyaluronic Acid Hydrogel Brackin, Riley B. McColgan, Gail E. Pucha, Saitheja A. Kowalski, Michael A. Drissi, Hicham Doan, Thanh N. Patel, Jay M. Bioengineering (Basel) Article Traumatic joint injuries are common, leading to progressive tissue degeneration and the development of osteoarthritis. The post-traumatic joint experiences a pro-inflammatory milieu, initiating a subtle but deteriorative process in cartilage tissue. To prevent or even reverse this process, our group previously developed a tissue-penetrating methacrylated hyaluronic acid (MeHA) hydrogel system, crosslinked within cartilage to restore and/or protect the tissue. In the current study, we further optimized this approach by investigating the impact of biomaterial molecular weight (MW; 20, 75, 100 kDa) on its integration within and reinforcement of cartilage, as well as its ability to protect tissue degradation in a catabolic state. Indeed, the low MW MeHA integrated and reinforced cartilage tissue better than the high MW counterparts. Furthermore, in a 2 week IL-1β explant culture model, the 20 kDa MeHA demonstrated the most protection from biphasic mechanical loss, best retention of proteoglycans (Safranin O staining), and least aggrecan breakdown (NITEGE). Thus, the lower MW MeHA gels integrated better into the tissue and provided the greatest protection of the cartilage matrix. Future work will test this formulation in a preclinical model, with the goal of translating this therapeutic approach for cartilage preservation. MDPI 2023-08-27 /pmc/articles/PMC10525634/ /pubmed/37760116 http://dx.doi.org/10.3390/bioengineering10091013 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Brackin, Riley B. McColgan, Gail E. Pucha, Saitheja A. Kowalski, Michael A. Drissi, Hicham Doan, Thanh N. Patel, Jay M. Improved Cartilage Protection with Low Molecular Weight Hyaluronic Acid Hydrogel |
title | Improved Cartilage Protection with Low Molecular Weight Hyaluronic Acid Hydrogel |
title_full | Improved Cartilage Protection with Low Molecular Weight Hyaluronic Acid Hydrogel |
title_fullStr | Improved Cartilage Protection with Low Molecular Weight Hyaluronic Acid Hydrogel |
title_full_unstemmed | Improved Cartilage Protection with Low Molecular Weight Hyaluronic Acid Hydrogel |
title_short | Improved Cartilage Protection with Low Molecular Weight Hyaluronic Acid Hydrogel |
title_sort | improved cartilage protection with low molecular weight hyaluronic acid hydrogel |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10525634/ https://www.ncbi.nlm.nih.gov/pubmed/37760116 http://dx.doi.org/10.3390/bioengineering10091013 |
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