Cargando…
Isolation, Identification, and Antibacterial Properties of Prodigiosin, a Bioactive Product Produced by a New Serratia marcescens JSSCPM1 Strain: Exploring the Biosynthetic Gene Clusters of Serratia Species for Biological Applications
Prodigiosin pigment has high medicinal value, so exploring this compound is a top priority. This report presents a prodigiosin bioactive compound isolated from Serratia marcescens JSSCPM1, a new strain. The purification process of this compound involves the application of different chromatographic m...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10526024/ https://www.ncbi.nlm.nih.gov/pubmed/37760761 http://dx.doi.org/10.3390/antibiotics12091466 |
_version_ | 1785110923815419904 |
---|---|
author | Arivuselvam, Rajaguru Dera, Ayed A. Parween Ali, Syed Alraey, Yasser Saif, Ahmed Hani, Umme Arumugam Ramakrishnan, Sivaa Azeeze, Mohamed Sheik Tharik Abdul Rajeshkumar, Raman Susil, Aishwarya Harindranath, Haritha Kumar, B. R. Prashantha |
author_facet | Arivuselvam, Rajaguru Dera, Ayed A. Parween Ali, Syed Alraey, Yasser Saif, Ahmed Hani, Umme Arumugam Ramakrishnan, Sivaa Azeeze, Mohamed Sheik Tharik Abdul Rajeshkumar, Raman Susil, Aishwarya Harindranath, Haritha Kumar, B. R. Prashantha |
author_sort | Arivuselvam, Rajaguru |
collection | PubMed |
description | Prodigiosin pigment has high medicinal value, so exploring this compound is a top priority. This report presents a prodigiosin bioactive compound isolated from Serratia marcescens JSSCPM1, a new strain. The purification process of this compound involves the application of different chromatographic methods, including UV-visible spectroscopy, high-performance liquid chromatography (HPLC), and liquid chromatography–mass spectrometry (LC/MS). Subsequent analysis was performed using nuclear magnetic resonance (NMR) to achieve a deeper understanding of the compound’s structure. Finally, through a comprehensive review of the existing literature, the structural composition of the isolated bioactive compound was found to correspond to that of the well-known compound prodigiosin. The isolated prodigiosin compound was screened for antibacterial activity against both Gram-positive and Gram-negative bacteria. The compound inhibited the growth of Gram-negative bacterial strains compared with Gram-positive bacterial strains. It showed a maximum minimum inhibitory concentration against Escherichia coli NCIM 2065 at a 15.9 ± 0.31 μg/mL concentration. The potential binding capabilities between prodigiosin and the OmpF porin proteins (4GCS, 4GCP, and 4GCQ) were determined using in silico studies, which are generally the primary targets of different antibiotics. Comparative molecular docking analysis indicated that prodigiosin exhibits a good binding affinity toward these selected drug targets. |
format | Online Article Text |
id | pubmed-10526024 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105260242023-09-28 Isolation, Identification, and Antibacterial Properties of Prodigiosin, a Bioactive Product Produced by a New Serratia marcescens JSSCPM1 Strain: Exploring the Biosynthetic Gene Clusters of Serratia Species for Biological Applications Arivuselvam, Rajaguru Dera, Ayed A. Parween Ali, Syed Alraey, Yasser Saif, Ahmed Hani, Umme Arumugam Ramakrishnan, Sivaa Azeeze, Mohamed Sheik Tharik Abdul Rajeshkumar, Raman Susil, Aishwarya Harindranath, Haritha Kumar, B. R. Prashantha Antibiotics (Basel) Article Prodigiosin pigment has high medicinal value, so exploring this compound is a top priority. This report presents a prodigiosin bioactive compound isolated from Serratia marcescens JSSCPM1, a new strain. The purification process of this compound involves the application of different chromatographic methods, including UV-visible spectroscopy, high-performance liquid chromatography (HPLC), and liquid chromatography–mass spectrometry (LC/MS). Subsequent analysis was performed using nuclear magnetic resonance (NMR) to achieve a deeper understanding of the compound’s structure. Finally, through a comprehensive review of the existing literature, the structural composition of the isolated bioactive compound was found to correspond to that of the well-known compound prodigiosin. The isolated prodigiosin compound was screened for antibacterial activity against both Gram-positive and Gram-negative bacteria. The compound inhibited the growth of Gram-negative bacterial strains compared with Gram-positive bacterial strains. It showed a maximum minimum inhibitory concentration against Escherichia coli NCIM 2065 at a 15.9 ± 0.31 μg/mL concentration. The potential binding capabilities between prodigiosin and the OmpF porin proteins (4GCS, 4GCP, and 4GCQ) were determined using in silico studies, which are generally the primary targets of different antibiotics. Comparative molecular docking analysis indicated that prodigiosin exhibits a good binding affinity toward these selected drug targets. MDPI 2023-09-20 /pmc/articles/PMC10526024/ /pubmed/37760761 http://dx.doi.org/10.3390/antibiotics12091466 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Arivuselvam, Rajaguru Dera, Ayed A. Parween Ali, Syed Alraey, Yasser Saif, Ahmed Hani, Umme Arumugam Ramakrishnan, Sivaa Azeeze, Mohamed Sheik Tharik Abdul Rajeshkumar, Raman Susil, Aishwarya Harindranath, Haritha Kumar, B. R. Prashantha Isolation, Identification, and Antibacterial Properties of Prodigiosin, a Bioactive Product Produced by a New Serratia marcescens JSSCPM1 Strain: Exploring the Biosynthetic Gene Clusters of Serratia Species for Biological Applications |
title | Isolation, Identification, and Antibacterial Properties of Prodigiosin, a Bioactive Product Produced by a New Serratia marcescens JSSCPM1 Strain: Exploring the Biosynthetic Gene Clusters of Serratia Species for Biological Applications |
title_full | Isolation, Identification, and Antibacterial Properties of Prodigiosin, a Bioactive Product Produced by a New Serratia marcescens JSSCPM1 Strain: Exploring the Biosynthetic Gene Clusters of Serratia Species for Biological Applications |
title_fullStr | Isolation, Identification, and Antibacterial Properties of Prodigiosin, a Bioactive Product Produced by a New Serratia marcescens JSSCPM1 Strain: Exploring the Biosynthetic Gene Clusters of Serratia Species for Biological Applications |
title_full_unstemmed | Isolation, Identification, and Antibacterial Properties of Prodigiosin, a Bioactive Product Produced by a New Serratia marcescens JSSCPM1 Strain: Exploring the Biosynthetic Gene Clusters of Serratia Species for Biological Applications |
title_short | Isolation, Identification, and Antibacterial Properties of Prodigiosin, a Bioactive Product Produced by a New Serratia marcescens JSSCPM1 Strain: Exploring the Biosynthetic Gene Clusters of Serratia Species for Biological Applications |
title_sort | isolation, identification, and antibacterial properties of prodigiosin, a bioactive product produced by a new serratia marcescens jsscpm1 strain: exploring the biosynthetic gene clusters of serratia species for biological applications |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10526024/ https://www.ncbi.nlm.nih.gov/pubmed/37760761 http://dx.doi.org/10.3390/antibiotics12091466 |
work_keys_str_mv | AT arivuselvamrajaguru isolationidentificationandantibacterialpropertiesofprodigiosinabioactiveproductproducedbyanewserratiamarcescensjsscpm1strainexploringthebiosyntheticgeneclustersofserratiaspeciesforbiologicalapplications AT deraayeda isolationidentificationandantibacterialpropertiesofprodigiosinabioactiveproductproducedbyanewserratiamarcescensjsscpm1strainexploringthebiosyntheticgeneclustersofserratiaspeciesforbiologicalapplications AT parweenalisyed isolationidentificationandantibacterialpropertiesofprodigiosinabioactiveproductproducedbyanewserratiamarcescensjsscpm1strainexploringthebiosyntheticgeneclustersofserratiaspeciesforbiologicalapplications AT alraeyyasser isolationidentificationandantibacterialpropertiesofprodigiosinabioactiveproductproducedbyanewserratiamarcescensjsscpm1strainexploringthebiosyntheticgeneclustersofserratiaspeciesforbiologicalapplications AT saifahmed isolationidentificationandantibacterialpropertiesofprodigiosinabioactiveproductproducedbyanewserratiamarcescensjsscpm1strainexploringthebiosyntheticgeneclustersofserratiaspeciesforbiologicalapplications AT haniumme isolationidentificationandantibacterialpropertiesofprodigiosinabioactiveproductproducedbyanewserratiamarcescensjsscpm1strainexploringthebiosyntheticgeneclustersofserratiaspeciesforbiologicalapplications AT arumugamramakrishnansivaa isolationidentificationandantibacterialpropertiesofprodigiosinabioactiveproductproducedbyanewserratiamarcescensjsscpm1strainexploringthebiosyntheticgeneclustersofserratiaspeciesforbiologicalapplications AT azeezemohamedsheiktharikabdul isolationidentificationandantibacterialpropertiesofprodigiosinabioactiveproductproducedbyanewserratiamarcescensjsscpm1strainexploringthebiosyntheticgeneclustersofserratiaspeciesforbiologicalapplications AT rajeshkumarraman isolationidentificationandantibacterialpropertiesofprodigiosinabioactiveproductproducedbyanewserratiamarcescensjsscpm1strainexploringthebiosyntheticgeneclustersofserratiaspeciesforbiologicalapplications AT susilaishwarya isolationidentificationandantibacterialpropertiesofprodigiosinabioactiveproductproducedbyanewserratiamarcescensjsscpm1strainexploringthebiosyntheticgeneclustersofserratiaspeciesforbiologicalapplications AT harindranathharitha isolationidentificationandantibacterialpropertiesofprodigiosinabioactiveproductproducedbyanewserratiamarcescensjsscpm1strainexploringthebiosyntheticgeneclustersofserratiaspeciesforbiologicalapplications AT kumarbrprashantha isolationidentificationandantibacterialpropertiesofprodigiosinabioactiveproductproducedbyanewserratiamarcescensjsscpm1strainexploringthebiosyntheticgeneclustersofserratiaspeciesforbiologicalapplications |