Epidemiological and Molecular Approaches for a Fatal Feline Panleukopenia Virus Infection of Captive Siberian Tigers (Panthera tigris altaica) in the Republic of Korea

SIMPLE SUMMARY: A fatal feline panleukopenia virus (FPV) infection occurred in endangered Siberian tigers living in a zoo in the Republic of Korea. Genetic and phylogenetic analyses revealed that the infection was caused by the cross-species transmission of cat-derived FPV prevalent in Korea. The vaccinated tigers were infected with FPV most likely due to insufficient protective immunity or vaccine failure triggered by maternal-derived antibody interference. Therefore, improved biosecurity and vaccination guidelines are required to save the lives of endangered wild carnivores from fatal FPV infection. The data presented in this study will help us expand our knowledge of the molecular epidemiology of FPV in Korea and establish an effective control plan to prevent future FPV infections in captive wild carnivores. ABSTRACT: Feline panleukopenia virus (FPV), a member of the species Protoparvovirus carnivoran1, is one of the most fatal pathogens of domestic and wild carnivores. The virus endemically infects domestic carnivores worldwide and its cross-species transmission threatens endangered wild carnivores, including Siberian tigers. In this study, a fatal FPV infection in endangered Siberian tigers was investigated to trace the origin of the virus and elucidate the reason behind FPV’s infection of the vaccinated tigers. Our genetic characterization and phylogenetic analysis revealed that the virus detected in the infected tigers, designated as the KTPV-2305 strain, was closely related to FPV strains circulating in Korean cats, suggesting that it might have been transmitted from stray cats wandering around the zoo. Compared with the prototype FPV reference strains, the KTPV-2305 strain carried three distinct amino acid (aa) mutations in the VP2 protein sequence (I101T, I232V, and L562V) in this study. These three mutations are commonly found in most global FPV strains, including Korean strains, indicating that these mutations are common evolutionary characteristics of currently circulating global FPVs. The reason why the vaccinated tigers were infected with FPV was most likely the insufficient protective immunity of the affected tigress or vaccine failure triggered by the interference of maternal-derived antibodies in the affected tiger cubs. These findings suggest that improved vaccination guidelines are urgently needed to save the lives of wild carnivores from this fatal virus.