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Chlorhexidine-Containing Electrospun Polymeric Nanofibers for Dental Applications: An In Vitro Study

Chlorhexidine is the most commonly used anti-infective drug in dentistry. To treat infected void areas, a drug-loaded material that swells to fill the void and releases the drug slowly is needed. This study investigated the encapsulation and release of chlorhexidine from cellulose acetate nanofibers...

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Autores principales: de Carvalho, Luana Dutra, Peres, Bernardo Urbanetto, Shen, Ya, Haapasalo, Markus, Maezono, Hazuki, Manso, Adriana P., Ko, Frank, Jackson, John, Carvalho, Ricardo M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10526102/
https://www.ncbi.nlm.nih.gov/pubmed/37760711
http://dx.doi.org/10.3390/antibiotics12091414
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author de Carvalho, Luana Dutra
Peres, Bernardo Urbanetto
Shen, Ya
Haapasalo, Markus
Maezono, Hazuki
Manso, Adriana P.
Ko, Frank
Jackson, John
Carvalho, Ricardo M.
author_facet de Carvalho, Luana Dutra
Peres, Bernardo Urbanetto
Shen, Ya
Haapasalo, Markus
Maezono, Hazuki
Manso, Adriana P.
Ko, Frank
Jackson, John
Carvalho, Ricardo M.
author_sort de Carvalho, Luana Dutra
collection PubMed
description Chlorhexidine is the most commonly used anti-infective drug in dentistry. To treat infected void areas, a drug-loaded material that swells to fill the void and releases the drug slowly is needed. This study investigated the encapsulation and release of chlorhexidine from cellulose acetate nanofibers for use as an antibacterial treatment for dental bacterial infections by oral bacteria Streptococcus mutans and Enterococcus faecalis. This study used a commercial electrospinning machine to finely control the manufacture of thin, flexible, chlorhexidine-loaded cellulose acetate nanofiber mats with very-small-diameter fibers (measured using SEM). Water absorption was measured gravimetrically, drug release was analyzed by absorbance at 254 nm, and antibiotic effects were measured by halo analysis in agar. Slow electrospinning at lower voltage (14 kV), short target distance (14 cm), slow traverse and rotation, and syringe injection speeds with controlled humidity and temperature allowed for the manufacture of strong, thin films with evenly cross-meshed, uniform low-diameter nanofibers (640 nm) that were flexible and absorbed over 600% in water. Chlorhexidine was encapsulated efficiently and released in a controlled manner. All formulations killed both bacteria and may be used to fill infected voids by swelling for intimate contact with surfaces and hold the drug in the swollen matrix for effective bacterial killing in dental settings.
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spelling pubmed-105261022023-09-28 Chlorhexidine-Containing Electrospun Polymeric Nanofibers for Dental Applications: An In Vitro Study de Carvalho, Luana Dutra Peres, Bernardo Urbanetto Shen, Ya Haapasalo, Markus Maezono, Hazuki Manso, Adriana P. Ko, Frank Jackson, John Carvalho, Ricardo M. Antibiotics (Basel) Article Chlorhexidine is the most commonly used anti-infective drug in dentistry. To treat infected void areas, a drug-loaded material that swells to fill the void and releases the drug slowly is needed. This study investigated the encapsulation and release of chlorhexidine from cellulose acetate nanofibers for use as an antibacterial treatment for dental bacterial infections by oral bacteria Streptococcus mutans and Enterococcus faecalis. This study used a commercial electrospinning machine to finely control the manufacture of thin, flexible, chlorhexidine-loaded cellulose acetate nanofiber mats with very-small-diameter fibers (measured using SEM). Water absorption was measured gravimetrically, drug release was analyzed by absorbance at 254 nm, and antibiotic effects were measured by halo analysis in agar. Slow electrospinning at lower voltage (14 kV), short target distance (14 cm), slow traverse and rotation, and syringe injection speeds with controlled humidity and temperature allowed for the manufacture of strong, thin films with evenly cross-meshed, uniform low-diameter nanofibers (640 nm) that were flexible and absorbed over 600% in water. Chlorhexidine was encapsulated efficiently and released in a controlled manner. All formulations killed both bacteria and may be used to fill infected voids by swelling for intimate contact with surfaces and hold the drug in the swollen matrix for effective bacterial killing in dental settings. MDPI 2023-09-06 /pmc/articles/PMC10526102/ /pubmed/37760711 http://dx.doi.org/10.3390/antibiotics12091414 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Carvalho, Luana Dutra
Peres, Bernardo Urbanetto
Shen, Ya
Haapasalo, Markus
Maezono, Hazuki
Manso, Adriana P.
Ko, Frank
Jackson, John
Carvalho, Ricardo M.
Chlorhexidine-Containing Electrospun Polymeric Nanofibers for Dental Applications: An In Vitro Study
title Chlorhexidine-Containing Electrospun Polymeric Nanofibers for Dental Applications: An In Vitro Study
title_full Chlorhexidine-Containing Electrospun Polymeric Nanofibers for Dental Applications: An In Vitro Study
title_fullStr Chlorhexidine-Containing Electrospun Polymeric Nanofibers for Dental Applications: An In Vitro Study
title_full_unstemmed Chlorhexidine-Containing Electrospun Polymeric Nanofibers for Dental Applications: An In Vitro Study
title_short Chlorhexidine-Containing Electrospun Polymeric Nanofibers for Dental Applications: An In Vitro Study
title_sort chlorhexidine-containing electrospun polymeric nanofibers for dental applications: an in vitro study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10526102/
https://www.ncbi.nlm.nih.gov/pubmed/37760711
http://dx.doi.org/10.3390/antibiotics12091414
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