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Revisiting Schistosoma mansoni Micro-Exon Gene (MEG) Protein Family: A Tour into Conserved Motifs and Annotation

Genome sequencing of the human parasite Schistosoma mansoni revealed an interesting gene superfamily, called micro-exon gene (meg), that encodes secreted MEG proteins. The genes are composed of short exons (3–81 base pairs) regularly interspersed with long introns (up to 5 kbp). This article recolle...

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Autores principales: Nedvědová, Štěpánka, De Stefano, Davide, Walker, Olivier, Hologne, Maggy, Miele, Adriana Erica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10526429/
https://www.ncbi.nlm.nih.gov/pubmed/37759676
http://dx.doi.org/10.3390/biom13091275
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author Nedvědová, Štěpánka
De Stefano, Davide
Walker, Olivier
Hologne, Maggy
Miele, Adriana Erica
author_facet Nedvědová, Štěpánka
De Stefano, Davide
Walker, Olivier
Hologne, Maggy
Miele, Adriana Erica
author_sort Nedvědová, Štěpánka
collection PubMed
description Genome sequencing of the human parasite Schistosoma mansoni revealed an interesting gene superfamily, called micro-exon gene (meg), that encodes secreted MEG proteins. The genes are composed of short exons (3–81 base pairs) regularly interspersed with long introns (up to 5 kbp). This article recollects 35 S. mansoni specific meg genes that are distributed over 7 autosomes and one pair of sex chromosomes and that code for at least 87 verified MEG proteins. We used various bioinformatics tools to produce an optimal alignment and propose a phylogenetic analysis. This work highlighted intriguing conserved patterns/motifs in the sequences of the highly variable MEG proteins. Based on the analyses, we were able to classify the verified MEG proteins into two subfamilies and to hypothesize their duplication and colonization of all the chromosomes. Together with motif identification, we also proposed to revisit MEGs’ common names and annotation in order to avoid duplication, to help the reproducibility of research results and to avoid possible misunderstandings.
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spelling pubmed-105264292023-09-28 Revisiting Schistosoma mansoni Micro-Exon Gene (MEG) Protein Family: A Tour into Conserved Motifs and Annotation Nedvědová, Štěpánka De Stefano, Davide Walker, Olivier Hologne, Maggy Miele, Adriana Erica Biomolecules Article Genome sequencing of the human parasite Schistosoma mansoni revealed an interesting gene superfamily, called micro-exon gene (meg), that encodes secreted MEG proteins. The genes are composed of short exons (3–81 base pairs) regularly interspersed with long introns (up to 5 kbp). This article recollects 35 S. mansoni specific meg genes that are distributed over 7 autosomes and one pair of sex chromosomes and that code for at least 87 verified MEG proteins. We used various bioinformatics tools to produce an optimal alignment and propose a phylogenetic analysis. This work highlighted intriguing conserved patterns/motifs in the sequences of the highly variable MEG proteins. Based on the analyses, we were able to classify the verified MEG proteins into two subfamilies and to hypothesize their duplication and colonization of all the chromosomes. Together with motif identification, we also proposed to revisit MEGs’ common names and annotation in order to avoid duplication, to help the reproducibility of research results and to avoid possible misunderstandings. MDPI 2023-08-22 /pmc/articles/PMC10526429/ /pubmed/37759676 http://dx.doi.org/10.3390/biom13091275 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nedvědová, Štěpánka
De Stefano, Davide
Walker, Olivier
Hologne, Maggy
Miele, Adriana Erica
Revisiting Schistosoma mansoni Micro-Exon Gene (MEG) Protein Family: A Tour into Conserved Motifs and Annotation
title Revisiting Schistosoma mansoni Micro-Exon Gene (MEG) Protein Family: A Tour into Conserved Motifs and Annotation
title_full Revisiting Schistosoma mansoni Micro-Exon Gene (MEG) Protein Family: A Tour into Conserved Motifs and Annotation
title_fullStr Revisiting Schistosoma mansoni Micro-Exon Gene (MEG) Protein Family: A Tour into Conserved Motifs and Annotation
title_full_unstemmed Revisiting Schistosoma mansoni Micro-Exon Gene (MEG) Protein Family: A Tour into Conserved Motifs and Annotation
title_short Revisiting Schistosoma mansoni Micro-Exon Gene (MEG) Protein Family: A Tour into Conserved Motifs and Annotation
title_sort revisiting schistosoma mansoni micro-exon gene (meg) protein family: a tour into conserved motifs and annotation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10526429/
https://www.ncbi.nlm.nih.gov/pubmed/37759676
http://dx.doi.org/10.3390/biom13091275
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