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Revisiting Schistosoma mansoni Micro-Exon Gene (MEG) Protein Family: A Tour into Conserved Motifs and Annotation
Genome sequencing of the human parasite Schistosoma mansoni revealed an interesting gene superfamily, called micro-exon gene (meg), that encodes secreted MEG proteins. The genes are composed of short exons (3–81 base pairs) regularly interspersed with long introns (up to 5 kbp). This article recolle...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10526429/ https://www.ncbi.nlm.nih.gov/pubmed/37759676 http://dx.doi.org/10.3390/biom13091275 |
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author | Nedvědová, Štěpánka De Stefano, Davide Walker, Olivier Hologne, Maggy Miele, Adriana Erica |
author_facet | Nedvědová, Štěpánka De Stefano, Davide Walker, Olivier Hologne, Maggy Miele, Adriana Erica |
author_sort | Nedvědová, Štěpánka |
collection | PubMed |
description | Genome sequencing of the human parasite Schistosoma mansoni revealed an interesting gene superfamily, called micro-exon gene (meg), that encodes secreted MEG proteins. The genes are composed of short exons (3–81 base pairs) regularly interspersed with long introns (up to 5 kbp). This article recollects 35 S. mansoni specific meg genes that are distributed over 7 autosomes and one pair of sex chromosomes and that code for at least 87 verified MEG proteins. We used various bioinformatics tools to produce an optimal alignment and propose a phylogenetic analysis. This work highlighted intriguing conserved patterns/motifs in the sequences of the highly variable MEG proteins. Based on the analyses, we were able to classify the verified MEG proteins into two subfamilies and to hypothesize their duplication and colonization of all the chromosomes. Together with motif identification, we also proposed to revisit MEGs’ common names and annotation in order to avoid duplication, to help the reproducibility of research results and to avoid possible misunderstandings. |
format | Online Article Text |
id | pubmed-10526429 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105264292023-09-28 Revisiting Schistosoma mansoni Micro-Exon Gene (MEG) Protein Family: A Tour into Conserved Motifs and Annotation Nedvědová, Štěpánka De Stefano, Davide Walker, Olivier Hologne, Maggy Miele, Adriana Erica Biomolecules Article Genome sequencing of the human parasite Schistosoma mansoni revealed an interesting gene superfamily, called micro-exon gene (meg), that encodes secreted MEG proteins. The genes are composed of short exons (3–81 base pairs) regularly interspersed with long introns (up to 5 kbp). This article recollects 35 S. mansoni specific meg genes that are distributed over 7 autosomes and one pair of sex chromosomes and that code for at least 87 verified MEG proteins. We used various bioinformatics tools to produce an optimal alignment and propose a phylogenetic analysis. This work highlighted intriguing conserved patterns/motifs in the sequences of the highly variable MEG proteins. Based on the analyses, we were able to classify the verified MEG proteins into two subfamilies and to hypothesize their duplication and colonization of all the chromosomes. Together with motif identification, we also proposed to revisit MEGs’ common names and annotation in order to avoid duplication, to help the reproducibility of research results and to avoid possible misunderstandings. MDPI 2023-08-22 /pmc/articles/PMC10526429/ /pubmed/37759676 http://dx.doi.org/10.3390/biom13091275 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nedvědová, Štěpánka De Stefano, Davide Walker, Olivier Hologne, Maggy Miele, Adriana Erica Revisiting Schistosoma mansoni Micro-Exon Gene (MEG) Protein Family: A Tour into Conserved Motifs and Annotation |
title | Revisiting Schistosoma mansoni Micro-Exon Gene (MEG) Protein Family: A Tour into Conserved Motifs and Annotation |
title_full | Revisiting Schistosoma mansoni Micro-Exon Gene (MEG) Protein Family: A Tour into Conserved Motifs and Annotation |
title_fullStr | Revisiting Schistosoma mansoni Micro-Exon Gene (MEG) Protein Family: A Tour into Conserved Motifs and Annotation |
title_full_unstemmed | Revisiting Schistosoma mansoni Micro-Exon Gene (MEG) Protein Family: A Tour into Conserved Motifs and Annotation |
title_short | Revisiting Schistosoma mansoni Micro-Exon Gene (MEG) Protein Family: A Tour into Conserved Motifs and Annotation |
title_sort | revisiting schistosoma mansoni micro-exon gene (meg) protein family: a tour into conserved motifs and annotation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10526429/ https://www.ncbi.nlm.nih.gov/pubmed/37759676 http://dx.doi.org/10.3390/biom13091275 |
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