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Recent Uses of Paper Microfluidics in Isothermal Nucleic Acid Amplification Tests
Isothermal nucleic acid amplification tests have recently gained popularity over polymerase chain reaction (PCR), as they only require a constant temperature and significantly simplify nucleic acid amplification. Recently, numerous attempts have been made to incorporate paper microfluidics into thes...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10526735/ https://www.ncbi.nlm.nih.gov/pubmed/37754119 http://dx.doi.org/10.3390/bios13090885 |
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author | Reynolds, Jocelyn Loeffler, Reid S. Leigh, Preston J. Lopez, Hannah A. Yoon, Jeong-Yeol |
author_facet | Reynolds, Jocelyn Loeffler, Reid S. Leigh, Preston J. Lopez, Hannah A. Yoon, Jeong-Yeol |
author_sort | Reynolds, Jocelyn |
collection | PubMed |
description | Isothermal nucleic acid amplification tests have recently gained popularity over polymerase chain reaction (PCR), as they only require a constant temperature and significantly simplify nucleic acid amplification. Recently, numerous attempts have been made to incorporate paper microfluidics into these isothermal amplification tests. Paper microfluidics (including lateral flow strips) have been used to extract nucleic acids, amplify the target gene, and detect amplified products, all toward automating the process. We investigated the literature from 2020 to the present, i.e., since the onset of the COVID-19 pandemic, during which a significant surge in isothermal amplification tests has been observed. Paper microfluidic detection has been used extensively for recombinase polymerase amplification (RPA) and its related methods, along with loop-mediated isothermal amplification (LAMP) and rolling circle amplification (RCA). Detection was conducted primarily with colorimetric and fluorometric methods, although a few publications demonstrated flow distance- and surface-enhanced Raman spectroscopic (SERS)-based detection. A good number of publications could be found that demonstrated both amplification and detection on paper microfluidic platforms. A small number of publications could be found that showed extraction or all three procedures (i.e., fully integrated systems) on paper microfluidic platforms, necessitating the need for future work. |
format | Online Article Text |
id | pubmed-10526735 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105267352023-09-28 Recent Uses of Paper Microfluidics in Isothermal Nucleic Acid Amplification Tests Reynolds, Jocelyn Loeffler, Reid S. Leigh, Preston J. Lopez, Hannah A. Yoon, Jeong-Yeol Biosensors (Basel) Review Isothermal nucleic acid amplification tests have recently gained popularity over polymerase chain reaction (PCR), as they only require a constant temperature and significantly simplify nucleic acid amplification. Recently, numerous attempts have been made to incorporate paper microfluidics into these isothermal amplification tests. Paper microfluidics (including lateral flow strips) have been used to extract nucleic acids, amplify the target gene, and detect amplified products, all toward automating the process. We investigated the literature from 2020 to the present, i.e., since the onset of the COVID-19 pandemic, during which a significant surge in isothermal amplification tests has been observed. Paper microfluidic detection has been used extensively for recombinase polymerase amplification (RPA) and its related methods, along with loop-mediated isothermal amplification (LAMP) and rolling circle amplification (RCA). Detection was conducted primarily with colorimetric and fluorometric methods, although a few publications demonstrated flow distance- and surface-enhanced Raman spectroscopic (SERS)-based detection. A good number of publications could be found that demonstrated both amplification and detection on paper microfluidic platforms. A small number of publications could be found that showed extraction or all three procedures (i.e., fully integrated systems) on paper microfluidic platforms, necessitating the need for future work. MDPI 2023-09-15 /pmc/articles/PMC10526735/ /pubmed/37754119 http://dx.doi.org/10.3390/bios13090885 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Reynolds, Jocelyn Loeffler, Reid S. Leigh, Preston J. Lopez, Hannah A. Yoon, Jeong-Yeol Recent Uses of Paper Microfluidics in Isothermal Nucleic Acid Amplification Tests |
title | Recent Uses of Paper Microfluidics in Isothermal Nucleic Acid Amplification Tests |
title_full | Recent Uses of Paper Microfluidics in Isothermal Nucleic Acid Amplification Tests |
title_fullStr | Recent Uses of Paper Microfluidics in Isothermal Nucleic Acid Amplification Tests |
title_full_unstemmed | Recent Uses of Paper Microfluidics in Isothermal Nucleic Acid Amplification Tests |
title_short | Recent Uses of Paper Microfluidics in Isothermal Nucleic Acid Amplification Tests |
title_sort | recent uses of paper microfluidics in isothermal nucleic acid amplification tests |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10526735/ https://www.ncbi.nlm.nih.gov/pubmed/37754119 http://dx.doi.org/10.3390/bios13090885 |
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