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Development of an Innovative Colorimetric DNA Biosensor Based on Sugar Measurement
The development of biosensors for target detection plays a crucial role in advancing various fields of bioscience. This work presents the development of a genosensor that exploits the colorimetric phenol—sulfuric acid sugar reaction for the detection of DNA, and RNA as specific targets, and DNA inte...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10526849/ https://www.ncbi.nlm.nih.gov/pubmed/37754087 http://dx.doi.org/10.3390/bios13090853 |
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author | El Aamri, Maliana Khalki, Yasmine Mohammadi, Hasna Amine, Aziz |
author_facet | El Aamri, Maliana Khalki, Yasmine Mohammadi, Hasna Amine, Aziz |
author_sort | El Aamri, Maliana |
collection | PubMed |
description | The development of biosensors for target detection plays a crucial role in advancing various fields of bioscience. This work presents the development of a genosensor that exploits the colorimetric phenol—sulfuric acid sugar reaction for the detection of DNA, and RNA as specific targets, and DNA intercalator molecules. The biosensor combines simplicity and reliability to create a novel bioassay for accurate and rapid analysis. A 96-well microplate based on a polystyrene polymer was used as the platform for an unmodified capture DNA immobilization via a silanization process and with (3-Aminopropyl) triethoxysilane (APTES). After that, a hybridization step was carried out to catch the target molecule, followed by adding phenol and sulfuric acid to quantify the amount of DNA or RNA sugar backbone. This reaction generated a yellow-orange color on the wells measured at 490 nm, which was proportional to the target concentration. Under the optimum conditions, a calibration curve was obtained for each target. The developed biosensor demonstrated high sensitivity, good selectivity, and linear response over a wide concentration range for DNA and RNA targets. Additionally, the biosensor was successfully employed for the detection of DNA intercalator agents that inhibited the hybridization of DNA complementary to the immobilized capture DNA. The developed biosensor offers a potential tool for sensitive and selective detection in various applications, including virus diagnosis, genetic analysis, pathogenic bacteria monitoring, and drug discovery. |
format | Online Article Text |
id | pubmed-10526849 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105268492023-09-28 Development of an Innovative Colorimetric DNA Biosensor Based on Sugar Measurement El Aamri, Maliana Khalki, Yasmine Mohammadi, Hasna Amine, Aziz Biosensors (Basel) Article The development of biosensors for target detection plays a crucial role in advancing various fields of bioscience. This work presents the development of a genosensor that exploits the colorimetric phenol—sulfuric acid sugar reaction for the detection of DNA, and RNA as specific targets, and DNA intercalator molecules. The biosensor combines simplicity and reliability to create a novel bioassay for accurate and rapid analysis. A 96-well microplate based on a polystyrene polymer was used as the platform for an unmodified capture DNA immobilization via a silanization process and with (3-Aminopropyl) triethoxysilane (APTES). After that, a hybridization step was carried out to catch the target molecule, followed by adding phenol and sulfuric acid to quantify the amount of DNA or RNA sugar backbone. This reaction generated a yellow-orange color on the wells measured at 490 nm, which was proportional to the target concentration. Under the optimum conditions, a calibration curve was obtained for each target. The developed biosensor demonstrated high sensitivity, good selectivity, and linear response over a wide concentration range for DNA and RNA targets. Additionally, the biosensor was successfully employed for the detection of DNA intercalator agents that inhibited the hybridization of DNA complementary to the immobilized capture DNA. The developed biosensor offers a potential tool for sensitive and selective detection in various applications, including virus diagnosis, genetic analysis, pathogenic bacteria monitoring, and drug discovery. MDPI 2023-08-28 /pmc/articles/PMC10526849/ /pubmed/37754087 http://dx.doi.org/10.3390/bios13090853 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article El Aamri, Maliana Khalki, Yasmine Mohammadi, Hasna Amine, Aziz Development of an Innovative Colorimetric DNA Biosensor Based on Sugar Measurement |
title | Development of an Innovative Colorimetric DNA Biosensor Based on Sugar Measurement |
title_full | Development of an Innovative Colorimetric DNA Biosensor Based on Sugar Measurement |
title_fullStr | Development of an Innovative Colorimetric DNA Biosensor Based on Sugar Measurement |
title_full_unstemmed | Development of an Innovative Colorimetric DNA Biosensor Based on Sugar Measurement |
title_short | Development of an Innovative Colorimetric DNA Biosensor Based on Sugar Measurement |
title_sort | development of an innovative colorimetric dna biosensor based on sugar measurement |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10526849/ https://www.ncbi.nlm.nih.gov/pubmed/37754087 http://dx.doi.org/10.3390/bios13090853 |
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