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Unraveling the Possibilities: Recent Progress in DNA Biosensing

Due to the advantages of its numerous modification sites, predictable structure, high thermal stability, and excellent biocompatibility, DNA is the ideal choice as a key component of biosensors. DNA biosensors offer significant advantages over existing bioanalytical techniques, addressing limitation...

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Detalles Bibliográficos
Autores principales: Yu, Meng, He, Tingli, Wang, Qianqian, Cui, Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10526863/
https://www.ncbi.nlm.nih.gov/pubmed/37754122
http://dx.doi.org/10.3390/bios13090889
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author Yu, Meng
He, Tingli
Wang, Qianqian
Cui, Cheng
author_facet Yu, Meng
He, Tingli
Wang, Qianqian
Cui, Cheng
author_sort Yu, Meng
collection PubMed
description Due to the advantages of its numerous modification sites, predictable structure, high thermal stability, and excellent biocompatibility, DNA is the ideal choice as a key component of biosensors. DNA biosensors offer significant advantages over existing bioanalytical techniques, addressing limitations in sensitivity, selectivity, and limit of detection. Consequently, they have attracted significant attention from researchers worldwide. Here, we exemplify four foundational categories of functional nucleic acids: aptamers, DNAzymes, i-motifs, and G-quadruplexes, from the perspective of the structure-driven functionality in constructing DNA biosensors. Furthermore, we provide a concise overview of the design and detection mechanisms employed in these DNA biosensors. Noteworthy advantages of DNA as a sensor component, including its programmable structure, reaction predictility, exceptional specificity, excellent sensitivity, and thermal stability, are highlighted. These characteristics contribute to the efficacy and reliability of DNA biosensors. Despite their great potential, challenges remain for the successful application of DNA biosensors, spanning storage and detection conditions, as well as associated costs. To overcome these limitations, we propose potential strategies that can be implemented to solve these issues. By offering these insights, we aim to inspire subsequent researchers in related fields.
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spelling pubmed-105268632023-09-28 Unraveling the Possibilities: Recent Progress in DNA Biosensing Yu, Meng He, Tingli Wang, Qianqian Cui, Cheng Biosensors (Basel) Review Due to the advantages of its numerous modification sites, predictable structure, high thermal stability, and excellent biocompatibility, DNA is the ideal choice as a key component of biosensors. DNA biosensors offer significant advantages over existing bioanalytical techniques, addressing limitations in sensitivity, selectivity, and limit of detection. Consequently, they have attracted significant attention from researchers worldwide. Here, we exemplify four foundational categories of functional nucleic acids: aptamers, DNAzymes, i-motifs, and G-quadruplexes, from the perspective of the structure-driven functionality in constructing DNA biosensors. Furthermore, we provide a concise overview of the design and detection mechanisms employed in these DNA biosensors. Noteworthy advantages of DNA as a sensor component, including its programmable structure, reaction predictility, exceptional specificity, excellent sensitivity, and thermal stability, are highlighted. These characteristics contribute to the efficacy and reliability of DNA biosensors. Despite their great potential, challenges remain for the successful application of DNA biosensors, spanning storage and detection conditions, as well as associated costs. To overcome these limitations, we propose potential strategies that can be implemented to solve these issues. By offering these insights, we aim to inspire subsequent researchers in related fields. MDPI 2023-09-18 /pmc/articles/PMC10526863/ /pubmed/37754122 http://dx.doi.org/10.3390/bios13090889 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Yu, Meng
He, Tingli
Wang, Qianqian
Cui, Cheng
Unraveling the Possibilities: Recent Progress in DNA Biosensing
title Unraveling the Possibilities: Recent Progress in DNA Biosensing
title_full Unraveling the Possibilities: Recent Progress in DNA Biosensing
title_fullStr Unraveling the Possibilities: Recent Progress in DNA Biosensing
title_full_unstemmed Unraveling the Possibilities: Recent Progress in DNA Biosensing
title_short Unraveling the Possibilities: Recent Progress in DNA Biosensing
title_sort unraveling the possibilities: recent progress in dna biosensing
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10526863/
https://www.ncbi.nlm.nih.gov/pubmed/37754122
http://dx.doi.org/10.3390/bios13090889
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