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Characterization of the Abracl-Expressing Cell Populations in the Embryonic Mammalian Telencephalon
Abracl (ABRA C-terminal-like protein) is a small, non-typical winged-helix protein that shares similarity with the C-terminal domain of the protein ABRA (Actin-Binding Rho-Activating protein). The role of Abracl in the cell remains elusive, although in cancer cells, it has been implicated in prolife...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10527439/ https://www.ncbi.nlm.nih.gov/pubmed/37759737 http://dx.doi.org/10.3390/biom13091337 |
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author | Troumpoukis, Dimitrios Vasileiou, Andreas Rafail Siskos, Nikistratos Stylianopoulou, Electra Ypsilantis, Petros Skavdis, George Grigoriou, Maria E. |
author_facet | Troumpoukis, Dimitrios Vasileiou, Andreas Rafail Siskos, Nikistratos Stylianopoulou, Electra Ypsilantis, Petros Skavdis, George Grigoriou, Maria E. |
author_sort | Troumpoukis, Dimitrios |
collection | PubMed |
description | Abracl (ABRA C-terminal-like protein) is a small, non-typical winged-helix protein that shares similarity with the C-terminal domain of the protein ABRA (Actin-Binding Rho-Activating protein). The role of Abracl in the cell remains elusive, although in cancer cells, it has been implicated in proliferation, migration and actin dynamics. Our previous study showed that Abracl mRNA was expressed in the dividing cells of the subpallial subventricular zone (SVZ), in the developing cortical plate (CP), and in the diencephalic SVZ; however, the molecular identities of the Abracl-expressing cell populations were not defined in that work. In this study, we use double immunofluorescence to characterize the expression of Abracl on sections of embryonic murine (E11.5-E18.5) and feline (E30/31-E33/34) telencephalon; to this end, we use a battery of well-known molecular markers of cycling (Ki67, Ascl1, Dlx2) or post-mitotic (Tubb3, Gad65/67, Lhx6 and Tbr1) cells. Our experiments show that Abracl protein has, compared to the mRNA, a broader expression domain, including, apart from proliferating cells of the subpallial and diencephalic SVZ, post-mitotic cells occupying the subpallial and pallial mantle (including the CP), as well as subpallial-derived migrating interneurons. Interestingly, in late embryonic developmental stages, Abracl was also transiently detected in major telencephalic fiber tracts. |
format | Online Article Text |
id | pubmed-10527439 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105274392023-09-28 Characterization of the Abracl-Expressing Cell Populations in the Embryonic Mammalian Telencephalon Troumpoukis, Dimitrios Vasileiou, Andreas Rafail Siskos, Nikistratos Stylianopoulou, Electra Ypsilantis, Petros Skavdis, George Grigoriou, Maria E. Biomolecules Article Abracl (ABRA C-terminal-like protein) is a small, non-typical winged-helix protein that shares similarity with the C-terminal domain of the protein ABRA (Actin-Binding Rho-Activating protein). The role of Abracl in the cell remains elusive, although in cancer cells, it has been implicated in proliferation, migration and actin dynamics. Our previous study showed that Abracl mRNA was expressed in the dividing cells of the subpallial subventricular zone (SVZ), in the developing cortical plate (CP), and in the diencephalic SVZ; however, the molecular identities of the Abracl-expressing cell populations were not defined in that work. In this study, we use double immunofluorescence to characterize the expression of Abracl on sections of embryonic murine (E11.5-E18.5) and feline (E30/31-E33/34) telencephalon; to this end, we use a battery of well-known molecular markers of cycling (Ki67, Ascl1, Dlx2) or post-mitotic (Tubb3, Gad65/67, Lhx6 and Tbr1) cells. Our experiments show that Abracl protein has, compared to the mRNA, a broader expression domain, including, apart from proliferating cells of the subpallial and diencephalic SVZ, post-mitotic cells occupying the subpallial and pallial mantle (including the CP), as well as subpallial-derived migrating interneurons. Interestingly, in late embryonic developmental stages, Abracl was also transiently detected in major telencephalic fiber tracts. MDPI 2023-08-31 /pmc/articles/PMC10527439/ /pubmed/37759737 http://dx.doi.org/10.3390/biom13091337 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Troumpoukis, Dimitrios Vasileiou, Andreas Rafail Siskos, Nikistratos Stylianopoulou, Electra Ypsilantis, Petros Skavdis, George Grigoriou, Maria E. Characterization of the Abracl-Expressing Cell Populations in the Embryonic Mammalian Telencephalon |
title | Characterization of the Abracl-Expressing Cell Populations in the Embryonic Mammalian Telencephalon |
title_full | Characterization of the Abracl-Expressing Cell Populations in the Embryonic Mammalian Telencephalon |
title_fullStr | Characterization of the Abracl-Expressing Cell Populations in the Embryonic Mammalian Telencephalon |
title_full_unstemmed | Characterization of the Abracl-Expressing Cell Populations in the Embryonic Mammalian Telencephalon |
title_short | Characterization of the Abracl-Expressing Cell Populations in the Embryonic Mammalian Telencephalon |
title_sort | characterization of the abracl-expressing cell populations in the embryonic mammalian telencephalon |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10527439/ https://www.ncbi.nlm.nih.gov/pubmed/37759737 http://dx.doi.org/10.3390/biom13091337 |
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