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Characterization of the Abracl-Expressing Cell Populations in the Embryonic Mammalian Telencephalon

Abracl (ABRA C-terminal-like protein) is a small, non-typical winged-helix protein that shares similarity with the C-terminal domain of the protein ABRA (Actin-Binding Rho-Activating protein). The role of Abracl in the cell remains elusive, although in cancer cells, it has been implicated in prolife...

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Autores principales: Troumpoukis, Dimitrios, Vasileiou, Andreas Rafail, Siskos, Nikistratos, Stylianopoulou, Electra, Ypsilantis, Petros, Skavdis, George, Grigoriou, Maria E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10527439/
https://www.ncbi.nlm.nih.gov/pubmed/37759737
http://dx.doi.org/10.3390/biom13091337
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author Troumpoukis, Dimitrios
Vasileiou, Andreas Rafail
Siskos, Nikistratos
Stylianopoulou, Electra
Ypsilantis, Petros
Skavdis, George
Grigoriou, Maria E.
author_facet Troumpoukis, Dimitrios
Vasileiou, Andreas Rafail
Siskos, Nikistratos
Stylianopoulou, Electra
Ypsilantis, Petros
Skavdis, George
Grigoriou, Maria E.
author_sort Troumpoukis, Dimitrios
collection PubMed
description Abracl (ABRA C-terminal-like protein) is a small, non-typical winged-helix protein that shares similarity with the C-terminal domain of the protein ABRA (Actin-Binding Rho-Activating protein). The role of Abracl in the cell remains elusive, although in cancer cells, it has been implicated in proliferation, migration and actin dynamics. Our previous study showed that Abracl mRNA was expressed in the dividing cells of the subpallial subventricular zone (SVZ), in the developing cortical plate (CP), and in the diencephalic SVZ; however, the molecular identities of the Abracl-expressing cell populations were not defined in that work. In this study, we use double immunofluorescence to characterize the expression of Abracl on sections of embryonic murine (E11.5-E18.5) and feline (E30/31-E33/34) telencephalon; to this end, we use a battery of well-known molecular markers of cycling (Ki67, Ascl1, Dlx2) or post-mitotic (Tubb3, Gad65/67, Lhx6 and Tbr1) cells. Our experiments show that Abracl protein has, compared to the mRNA, a broader expression domain, including, apart from proliferating cells of the subpallial and diencephalic SVZ, post-mitotic cells occupying the subpallial and pallial mantle (including the CP), as well as subpallial-derived migrating interneurons. Interestingly, in late embryonic developmental stages, Abracl was also transiently detected in major telencephalic fiber tracts.
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spelling pubmed-105274392023-09-28 Characterization of the Abracl-Expressing Cell Populations in the Embryonic Mammalian Telencephalon Troumpoukis, Dimitrios Vasileiou, Andreas Rafail Siskos, Nikistratos Stylianopoulou, Electra Ypsilantis, Petros Skavdis, George Grigoriou, Maria E. Biomolecules Article Abracl (ABRA C-terminal-like protein) is a small, non-typical winged-helix protein that shares similarity with the C-terminal domain of the protein ABRA (Actin-Binding Rho-Activating protein). The role of Abracl in the cell remains elusive, although in cancer cells, it has been implicated in proliferation, migration and actin dynamics. Our previous study showed that Abracl mRNA was expressed in the dividing cells of the subpallial subventricular zone (SVZ), in the developing cortical plate (CP), and in the diencephalic SVZ; however, the molecular identities of the Abracl-expressing cell populations were not defined in that work. In this study, we use double immunofluorescence to characterize the expression of Abracl on sections of embryonic murine (E11.5-E18.5) and feline (E30/31-E33/34) telencephalon; to this end, we use a battery of well-known molecular markers of cycling (Ki67, Ascl1, Dlx2) or post-mitotic (Tubb3, Gad65/67, Lhx6 and Tbr1) cells. Our experiments show that Abracl protein has, compared to the mRNA, a broader expression domain, including, apart from proliferating cells of the subpallial and diencephalic SVZ, post-mitotic cells occupying the subpallial and pallial mantle (including the CP), as well as subpallial-derived migrating interneurons. Interestingly, in late embryonic developmental stages, Abracl was also transiently detected in major telencephalic fiber tracts. MDPI 2023-08-31 /pmc/articles/PMC10527439/ /pubmed/37759737 http://dx.doi.org/10.3390/biom13091337 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Troumpoukis, Dimitrios
Vasileiou, Andreas Rafail
Siskos, Nikistratos
Stylianopoulou, Electra
Ypsilantis, Petros
Skavdis, George
Grigoriou, Maria E.
Characterization of the Abracl-Expressing Cell Populations in the Embryonic Mammalian Telencephalon
title Characterization of the Abracl-Expressing Cell Populations in the Embryonic Mammalian Telencephalon
title_full Characterization of the Abracl-Expressing Cell Populations in the Embryonic Mammalian Telencephalon
title_fullStr Characterization of the Abracl-Expressing Cell Populations in the Embryonic Mammalian Telencephalon
title_full_unstemmed Characterization of the Abracl-Expressing Cell Populations in the Embryonic Mammalian Telencephalon
title_short Characterization of the Abracl-Expressing Cell Populations in the Embryonic Mammalian Telencephalon
title_sort characterization of the abracl-expressing cell populations in the embryonic mammalian telencephalon
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10527439/
https://www.ncbi.nlm.nih.gov/pubmed/37759737
http://dx.doi.org/10.3390/biom13091337
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