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Temporal Stability of Ciliary Beating Post Nasal Brushing, Modulated by Storage Temperature

Primary ciliary dyskinesia is a heterogeneous, inherited motile ciliopathy in which respiratory cilia beat abnormally, and some ultrastructural ciliary defects and specific genetic mutations have been associated with particular ciliary beating alterations. Ciliary beating can be evaluated using digi...

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Autores principales: Bricmont, Noemie, Bonhiver, Romane, Benchimol, Lionel, Louis, Bruno, Papon, Jean-François, Monseur, Justine, Donneau, Anne-Françoise, Moermans, Catherine, Schleich, Florence, Calmès, Doriane, Poirrier, Anne-Lise, Louis, Renaud, Seghaye, Marie-Christine, Kempeneers, Céline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10527713/
https://www.ncbi.nlm.nih.gov/pubmed/37761341
http://dx.doi.org/10.3390/diagnostics13182974
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author Bricmont, Noemie
Bonhiver, Romane
Benchimol, Lionel
Louis, Bruno
Papon, Jean-François
Monseur, Justine
Donneau, Anne-Françoise
Moermans, Catherine
Schleich, Florence
Calmès, Doriane
Poirrier, Anne-Lise
Louis, Renaud
Seghaye, Marie-Christine
Kempeneers, Céline
author_facet Bricmont, Noemie
Bonhiver, Romane
Benchimol, Lionel
Louis, Bruno
Papon, Jean-François
Monseur, Justine
Donneau, Anne-Françoise
Moermans, Catherine
Schleich, Florence
Calmès, Doriane
Poirrier, Anne-Lise
Louis, Renaud
Seghaye, Marie-Christine
Kempeneers, Céline
author_sort Bricmont, Noemie
collection PubMed
description Primary ciliary dyskinesia is a heterogeneous, inherited motile ciliopathy in which respiratory cilia beat abnormally, and some ultrastructural ciliary defects and specific genetic mutations have been associated with particular ciliary beating alterations. Ciliary beating can be evaluated using digital high-speed videomicroscopy (DHSV). However, normal reference values, essential to assess ciliary beating in patients referred for a PCD diagnostic, vary between centres, as minor variations in protocols might influence ciliary beating. Consequently, establishment of normal values is essential for each PCD diagnostic centre. We aimed to evaluate whether delay after sampling, and temperature for conservation of respiratory ciliated samples, might modify assessments of ciliary beating. In total, 37 healthy nasal brushing samples of respiratory ciliated epithelia were collected. Video sequences were recorded at 37 °C immediately using DHSV. Then, the samples were divided and conserved at 4 °C or at room temperature (RT). Ciliated beating edges were then recorded at 37 °C, at 3 h and at 9 h post sampling. In six samples, recordings were continued up to 72 h after sampling. Ciliary beating was assessed manually by ciliary beat frequency (CBF(M)) and ciliary beat pattern (CBP). A semi-automatic software was used for quantitative analysis. Both CBF and CBP evaluated manually and by a semi-automated method were stable 9 h after sampling. CBF(M) was higher when evaluated using samples stored at RT than at 4 °C. CBP and the semi-automated evaluation of ciliary beating were not affected by storage temperature. When establishing normal references values, ciliary beating can be evaluated at 37 °C up to 9 h after nasal brushing, but the storage temperature modifies ciliary beating and needs to be controlled.
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spelling pubmed-105277132023-09-28 Temporal Stability of Ciliary Beating Post Nasal Brushing, Modulated by Storage Temperature Bricmont, Noemie Bonhiver, Romane Benchimol, Lionel Louis, Bruno Papon, Jean-François Monseur, Justine Donneau, Anne-Françoise Moermans, Catherine Schleich, Florence Calmès, Doriane Poirrier, Anne-Lise Louis, Renaud Seghaye, Marie-Christine Kempeneers, Céline Diagnostics (Basel) Article Primary ciliary dyskinesia is a heterogeneous, inherited motile ciliopathy in which respiratory cilia beat abnormally, and some ultrastructural ciliary defects and specific genetic mutations have been associated with particular ciliary beating alterations. Ciliary beating can be evaluated using digital high-speed videomicroscopy (DHSV). However, normal reference values, essential to assess ciliary beating in patients referred for a PCD diagnostic, vary between centres, as minor variations in protocols might influence ciliary beating. Consequently, establishment of normal values is essential for each PCD diagnostic centre. We aimed to evaluate whether delay after sampling, and temperature for conservation of respiratory ciliated samples, might modify assessments of ciliary beating. In total, 37 healthy nasal brushing samples of respiratory ciliated epithelia were collected. Video sequences were recorded at 37 °C immediately using DHSV. Then, the samples were divided and conserved at 4 °C or at room temperature (RT). Ciliated beating edges were then recorded at 37 °C, at 3 h and at 9 h post sampling. In six samples, recordings were continued up to 72 h after sampling. Ciliary beating was assessed manually by ciliary beat frequency (CBF(M)) and ciliary beat pattern (CBP). A semi-automatic software was used for quantitative analysis. Both CBF and CBP evaluated manually and by a semi-automated method were stable 9 h after sampling. CBF(M) was higher when evaluated using samples stored at RT than at 4 °C. CBP and the semi-automated evaluation of ciliary beating were not affected by storage temperature. When establishing normal references values, ciliary beating can be evaluated at 37 °C up to 9 h after nasal brushing, but the storage temperature modifies ciliary beating and needs to be controlled. MDPI 2023-09-18 /pmc/articles/PMC10527713/ /pubmed/37761341 http://dx.doi.org/10.3390/diagnostics13182974 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bricmont, Noemie
Bonhiver, Romane
Benchimol, Lionel
Louis, Bruno
Papon, Jean-François
Monseur, Justine
Donneau, Anne-Françoise
Moermans, Catherine
Schleich, Florence
Calmès, Doriane
Poirrier, Anne-Lise
Louis, Renaud
Seghaye, Marie-Christine
Kempeneers, Céline
Temporal Stability of Ciliary Beating Post Nasal Brushing, Modulated by Storage Temperature
title Temporal Stability of Ciliary Beating Post Nasal Brushing, Modulated by Storage Temperature
title_full Temporal Stability of Ciliary Beating Post Nasal Brushing, Modulated by Storage Temperature
title_fullStr Temporal Stability of Ciliary Beating Post Nasal Brushing, Modulated by Storage Temperature
title_full_unstemmed Temporal Stability of Ciliary Beating Post Nasal Brushing, Modulated by Storage Temperature
title_short Temporal Stability of Ciliary Beating Post Nasal Brushing, Modulated by Storage Temperature
title_sort temporal stability of ciliary beating post nasal brushing, modulated by storage temperature
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10527713/
https://www.ncbi.nlm.nih.gov/pubmed/37761341
http://dx.doi.org/10.3390/diagnostics13182974
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