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The role of cannabinoid CB1 receptors in the antinociceptive and reparative actions of mesenchymal stem cells in rats with peripheral neuropathic pain

Mesenchymal stem cells (MSCs) can produce antinociceptive and reparative effects. Presumably, the MSCs‐induced antinociception may be partly due to the involvement of the endocannabinoid system. The study aimed to evaluate the antinociceptive and reparative effects of adipose‐derived MSCs (ADMSCs) u...

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Autores principales: Yerofeyeva, Anna‐Maria V., Pinchuk, Sergey V., Rjabceva, Svetlana N., Molchanova, Alla Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10527798/
https://www.ncbi.nlm.nih.gov/pubmed/37786759
http://dx.doi.org/10.1002/ibra.12129
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author Yerofeyeva, Anna‐Maria V.
Pinchuk, Sergey V.
Rjabceva, Svetlana N.
Molchanova, Alla Y.
author_facet Yerofeyeva, Anna‐Maria V.
Pinchuk, Sergey V.
Rjabceva, Svetlana N.
Molchanova, Alla Y.
author_sort Yerofeyeva, Anna‐Maria V.
collection PubMed
description Mesenchymal stem cells (MSCs) can produce antinociceptive and reparative effects. Presumably, the MSCs‐induced antinociception may be partly due to the involvement of the endocannabinoid system. The study aimed to evaluate the antinociceptive and reparative effects of adipose‐derived MSCs (ADMSCs) upon pharmacological modulation of cannabinoid CB(1) receptor in peripheral tissues or on ADMSCs' membranes in a rat model of peripheral neuropathy. ADMSCs were injected into the area of rat sciatic nerve injury (i) with no additional treatments, (ii) at the tissue CB(1) receptor activation by endogenous agonist anandamide (AEA) or blockade with a selective AM251 antagonist; and (iii) preincubated with AEA or AM251. The evaluation of CB(1) receptor activity involved analyzing nociceptive responses, gait parameters, and histology. Transplantation of ADMSCs upon activation of CB(1) receptors, both on AMSCs' membranes or in the area of nerve injury, accelerated the analgesia and recovery of dynamic gait parameters, abolished static gait disturbances, and promoted the fastest nerve regeneration. Only blockade of CB(1) receptors on ADMSCs shortened ADMSCs‐induced analgesia and decreased the number of preserved nerve fibers. CB(1) receptors on ADMSCs significantly contribute to their pain‐relieving and tissue‐repairing capabilities by stimulating the growth factors secretion and suppressing the release of pro‐inflammatory cytokines. Peripheral CB(1) receptors do not significantly influence ADMSC‐induced antinociception.
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spelling pubmed-105277982023-10-02 The role of cannabinoid CB1 receptors in the antinociceptive and reparative actions of mesenchymal stem cells in rats with peripheral neuropathic pain Yerofeyeva, Anna‐Maria V. Pinchuk, Sergey V. Rjabceva, Svetlana N. Molchanova, Alla Y. Ibrain Original Articles Mesenchymal stem cells (MSCs) can produce antinociceptive and reparative effects. Presumably, the MSCs‐induced antinociception may be partly due to the involvement of the endocannabinoid system. The study aimed to evaluate the antinociceptive and reparative effects of adipose‐derived MSCs (ADMSCs) upon pharmacological modulation of cannabinoid CB(1) receptor in peripheral tissues or on ADMSCs' membranes in a rat model of peripheral neuropathy. ADMSCs were injected into the area of rat sciatic nerve injury (i) with no additional treatments, (ii) at the tissue CB(1) receptor activation by endogenous agonist anandamide (AEA) or blockade with a selective AM251 antagonist; and (iii) preincubated with AEA or AM251. The evaluation of CB(1) receptor activity involved analyzing nociceptive responses, gait parameters, and histology. Transplantation of ADMSCs upon activation of CB(1) receptors, both on AMSCs' membranes or in the area of nerve injury, accelerated the analgesia and recovery of dynamic gait parameters, abolished static gait disturbances, and promoted the fastest nerve regeneration. Only blockade of CB(1) receptors on ADMSCs shortened ADMSCs‐induced analgesia and decreased the number of preserved nerve fibers. CB(1) receptors on ADMSCs significantly contribute to their pain‐relieving and tissue‐repairing capabilities by stimulating the growth factors secretion and suppressing the release of pro‐inflammatory cytokines. Peripheral CB(1) receptors do not significantly influence ADMSC‐induced antinociception. John Wiley and Sons Inc. 2023-08-24 /pmc/articles/PMC10527798/ /pubmed/37786759 http://dx.doi.org/10.1002/ibra.12129 Text en © 2023 The Authors. Ibrain published by Affiliated Hospital of Zunyi Medical University (AHZMU) and Wiley‐VCH GmbH. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Yerofeyeva, Anna‐Maria V.
Pinchuk, Sergey V.
Rjabceva, Svetlana N.
Molchanova, Alla Y.
The role of cannabinoid CB1 receptors in the antinociceptive and reparative actions of mesenchymal stem cells in rats with peripheral neuropathic pain
title The role of cannabinoid CB1 receptors in the antinociceptive and reparative actions of mesenchymal stem cells in rats with peripheral neuropathic pain
title_full The role of cannabinoid CB1 receptors in the antinociceptive and reparative actions of mesenchymal stem cells in rats with peripheral neuropathic pain
title_fullStr The role of cannabinoid CB1 receptors in the antinociceptive and reparative actions of mesenchymal stem cells in rats with peripheral neuropathic pain
title_full_unstemmed The role of cannabinoid CB1 receptors in the antinociceptive and reparative actions of mesenchymal stem cells in rats with peripheral neuropathic pain
title_short The role of cannabinoid CB1 receptors in the antinociceptive and reparative actions of mesenchymal stem cells in rats with peripheral neuropathic pain
title_sort role of cannabinoid cb1 receptors in the antinociceptive and reparative actions of mesenchymal stem cells in rats with peripheral neuropathic pain
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10527798/
https://www.ncbi.nlm.nih.gov/pubmed/37786759
http://dx.doi.org/10.1002/ibra.12129
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