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Dose-Dependent Shift in Relative Contribution of Homologous Recombination to DNA Repair after Low-LET Ionizing Radiation Exposure: Empirical Evidence and Numerical Simulation

Understanding the relative contributions of different repair pathways to radiation-induced DNA damage responses remains a challenging issue in terms of studying the radiation injury endpoints. The comparative manifestation of homologous recombination (HR) after irradiation with different doses great...

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Autores principales: Belov, Oleg, Chigasova, Anna, Pustovalova, Margarita, Osipov, Andrey, Eremin, Petr, Vorobyeva, Natalia, Osipov, Andreyan N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10528584/
https://www.ncbi.nlm.nih.gov/pubmed/37754249
http://dx.doi.org/10.3390/cimb45090465
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author Belov, Oleg
Chigasova, Anna
Pustovalova, Margarita
Osipov, Andrey
Eremin, Petr
Vorobyeva, Natalia
Osipov, Andreyan N.
author_facet Belov, Oleg
Chigasova, Anna
Pustovalova, Margarita
Osipov, Andrey
Eremin, Petr
Vorobyeva, Natalia
Osipov, Andreyan N.
author_sort Belov, Oleg
collection PubMed
description Understanding the relative contributions of different repair pathways to radiation-induced DNA damage responses remains a challenging issue in terms of studying the radiation injury endpoints. The comparative manifestation of homologous recombination (HR) after irradiation with different doses greatly determines the overall effectiveness of recovery in a dividing cell after irradiation, since HR is an error-free mechanism intended to perform the repair of DNA double-strand breaks (DSB) during S/G2 phases of the cell cycle. In this article, we present experimentally observed evidence of dose-dependent shifts in the relative contributions of HR in human fibroblasts after X-ray exposure at doses in the range 20–1000 mGy, which is also supported by quantitative modeling of DNA DSB repair. Our findings indicate that the increase in the radiation dose leads to a dose-dependent decrease in the relative contribution of HR in the entire repair process.
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spelling pubmed-105285842023-09-28 Dose-Dependent Shift in Relative Contribution of Homologous Recombination to DNA Repair after Low-LET Ionizing Radiation Exposure: Empirical Evidence and Numerical Simulation Belov, Oleg Chigasova, Anna Pustovalova, Margarita Osipov, Andrey Eremin, Petr Vorobyeva, Natalia Osipov, Andreyan N. Curr Issues Mol Biol Article Understanding the relative contributions of different repair pathways to radiation-induced DNA damage responses remains a challenging issue in terms of studying the radiation injury endpoints. The comparative manifestation of homologous recombination (HR) after irradiation with different doses greatly determines the overall effectiveness of recovery in a dividing cell after irradiation, since HR is an error-free mechanism intended to perform the repair of DNA double-strand breaks (DSB) during S/G2 phases of the cell cycle. In this article, we present experimentally observed evidence of dose-dependent shifts in the relative contributions of HR in human fibroblasts after X-ray exposure at doses in the range 20–1000 mGy, which is also supported by quantitative modeling of DNA DSB repair. Our findings indicate that the increase in the radiation dose leads to a dose-dependent decrease in the relative contribution of HR in the entire repair process. MDPI 2023-09-09 /pmc/articles/PMC10528584/ /pubmed/37754249 http://dx.doi.org/10.3390/cimb45090465 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Belov, Oleg
Chigasova, Anna
Pustovalova, Margarita
Osipov, Andrey
Eremin, Petr
Vorobyeva, Natalia
Osipov, Andreyan N.
Dose-Dependent Shift in Relative Contribution of Homologous Recombination to DNA Repair after Low-LET Ionizing Radiation Exposure: Empirical Evidence and Numerical Simulation
title Dose-Dependent Shift in Relative Contribution of Homologous Recombination to DNA Repair after Low-LET Ionizing Radiation Exposure: Empirical Evidence and Numerical Simulation
title_full Dose-Dependent Shift in Relative Contribution of Homologous Recombination to DNA Repair after Low-LET Ionizing Radiation Exposure: Empirical Evidence and Numerical Simulation
title_fullStr Dose-Dependent Shift in Relative Contribution of Homologous Recombination to DNA Repair after Low-LET Ionizing Radiation Exposure: Empirical Evidence and Numerical Simulation
title_full_unstemmed Dose-Dependent Shift in Relative Contribution of Homologous Recombination to DNA Repair after Low-LET Ionizing Radiation Exposure: Empirical Evidence and Numerical Simulation
title_short Dose-Dependent Shift in Relative Contribution of Homologous Recombination to DNA Repair after Low-LET Ionizing Radiation Exposure: Empirical Evidence and Numerical Simulation
title_sort dose-dependent shift in relative contribution of homologous recombination to dna repair after low-let ionizing radiation exposure: empirical evidence and numerical simulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10528584/
https://www.ncbi.nlm.nih.gov/pubmed/37754249
http://dx.doi.org/10.3390/cimb45090465
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