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Tracking B cell responses to the SARS-CoV-2 mRNA-1273 vaccine
Protective immunity following vaccination is sustained by long-lived antibody-secreting cells and resting memory B cells (MBCs). Responses to two-dose SARS-CoV-2 mRNA-1273 vaccination are evaluated longitudinally by multimodal single-cell analysis in three infection-naıïve individuals. Integrated su...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10529190/ https://www.ncbi.nlm.nih.gov/pubmed/37440409 http://dx.doi.org/10.1016/j.celrep.2023.112780 |
Sumario: | Protective immunity following vaccination is sustained by long-lived antibody-secreting cells and resting memory B cells (MBCs). Responses to two-dose SARS-CoV-2 mRNA-1273 vaccination are evaluated longitudinally by multimodal single-cell analysis in three infection-naıïve individuals. Integrated surface protein, transcriptomics, and B cell receptor (BCR) repertoire analysis of sorted plasmablasts and spike(+) (S-2P(+)) and S-2P(−) B cells reveal clonal expansion and accumulating mutations among S-2P(+) cells. These cells are enriched in a cluster of immunoglobulin G-expressing MBCs and evolve along a bifurcated trajectory rooted in CXCR3(+) MBCs. One branch leads to CD11c(+) atypical MBCs while the other develops from CD71(+) activated precursors to resting MBCs, the dominant population at month 6. Among 12 evolving S-2P(+) clones, several are populated with plasmablasts at early timepoints as well as CD71(+) activated and resting MBCs at later timepoints, and display intra- and/or inter-cohort BCR convergence. These relationships suggest a coordinated and predictable evolution of SARS-CoV-2 vaccine-generated MBCs. |
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