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Exploring the Transcriptome Dynamics of In Vivo Theileria annulata Infection in Crossbred Cattle

The molecular changes occurring in the host in response to in vivo Theileria annulata parasitic infection are not well understood. Therefore, the present study investigated the differential expression profiles of peripheral blood mononuclear cells (PBMCs) across Theileria annulata-infected and non-i...

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Autores principales: Ahlawat, Sonika, Choudhary, Vikas, Arora, Reena, Kumar, Ashish, Kaur, Mandeep, Chhabra, Pooja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10530335/
https://www.ncbi.nlm.nih.gov/pubmed/37761803
http://dx.doi.org/10.3390/genes14091663
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author Ahlawat, Sonika
Choudhary, Vikas
Arora, Reena
Kumar, Ashish
Kaur, Mandeep
Chhabra, Pooja
author_facet Ahlawat, Sonika
Choudhary, Vikas
Arora, Reena
Kumar, Ashish
Kaur, Mandeep
Chhabra, Pooja
author_sort Ahlawat, Sonika
collection PubMed
description The molecular changes occurring in the host in response to in vivo Theileria annulata parasitic infection are not well understood. Therefore, the present study investigated the differential expression profiles of peripheral blood mononuclear cells (PBMCs) across Theileria annulata-infected and non-infected crossbred cows. The differential expression profiles from PBMCs of infected and non-infected crossbred cows were generated by RNA sequencing. A marked difference in the expression of genes associated with innate immunity (FTH1, ACTB, ISG15) was observed between the two groups. The over-represented pathways in Theileria annulata-infected cows were associated with the immune system and regulation of the mitotic cycle. Enriched genes and pathways in non-infected animals were associated with the maintenance of chromatin integrity and cell structure. The highly connected genes identified in this study form potential candidates for further investigation into host–parasite interactions in cattle. An improved understanding of the transcriptomic dynamics during theileriosis would lead to underpinning molecular level differences related to the health status of cattle.
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spelling pubmed-105303352023-09-28 Exploring the Transcriptome Dynamics of In Vivo Theileria annulata Infection in Crossbred Cattle Ahlawat, Sonika Choudhary, Vikas Arora, Reena Kumar, Ashish Kaur, Mandeep Chhabra, Pooja Genes (Basel) Article The molecular changes occurring in the host in response to in vivo Theileria annulata parasitic infection are not well understood. Therefore, the present study investigated the differential expression profiles of peripheral blood mononuclear cells (PBMCs) across Theileria annulata-infected and non-infected crossbred cows. The differential expression profiles from PBMCs of infected and non-infected crossbred cows were generated by RNA sequencing. A marked difference in the expression of genes associated with innate immunity (FTH1, ACTB, ISG15) was observed between the two groups. The over-represented pathways in Theileria annulata-infected cows were associated with the immune system and regulation of the mitotic cycle. Enriched genes and pathways in non-infected animals were associated with the maintenance of chromatin integrity and cell structure. The highly connected genes identified in this study form potential candidates for further investigation into host–parasite interactions in cattle. An improved understanding of the transcriptomic dynamics during theileriosis would lead to underpinning molecular level differences related to the health status of cattle. MDPI 2023-08-22 /pmc/articles/PMC10530335/ /pubmed/37761803 http://dx.doi.org/10.3390/genes14091663 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ahlawat, Sonika
Choudhary, Vikas
Arora, Reena
Kumar, Ashish
Kaur, Mandeep
Chhabra, Pooja
Exploring the Transcriptome Dynamics of In Vivo Theileria annulata Infection in Crossbred Cattle
title Exploring the Transcriptome Dynamics of In Vivo Theileria annulata Infection in Crossbred Cattle
title_full Exploring the Transcriptome Dynamics of In Vivo Theileria annulata Infection in Crossbred Cattle
title_fullStr Exploring the Transcriptome Dynamics of In Vivo Theileria annulata Infection in Crossbred Cattle
title_full_unstemmed Exploring the Transcriptome Dynamics of In Vivo Theileria annulata Infection in Crossbred Cattle
title_short Exploring the Transcriptome Dynamics of In Vivo Theileria annulata Infection in Crossbred Cattle
title_sort exploring the transcriptome dynamics of in vivo theileria annulata infection in crossbred cattle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10530335/
https://www.ncbi.nlm.nih.gov/pubmed/37761803
http://dx.doi.org/10.3390/genes14091663
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