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Exploring the Transcriptome Dynamics of In Vivo Theileria annulata Infection in Crossbred Cattle
The molecular changes occurring in the host in response to in vivo Theileria annulata parasitic infection are not well understood. Therefore, the present study investigated the differential expression profiles of peripheral blood mononuclear cells (PBMCs) across Theileria annulata-infected and non-i...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10530335/ https://www.ncbi.nlm.nih.gov/pubmed/37761803 http://dx.doi.org/10.3390/genes14091663 |
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author | Ahlawat, Sonika Choudhary, Vikas Arora, Reena Kumar, Ashish Kaur, Mandeep Chhabra, Pooja |
author_facet | Ahlawat, Sonika Choudhary, Vikas Arora, Reena Kumar, Ashish Kaur, Mandeep Chhabra, Pooja |
author_sort | Ahlawat, Sonika |
collection | PubMed |
description | The molecular changes occurring in the host in response to in vivo Theileria annulata parasitic infection are not well understood. Therefore, the present study investigated the differential expression profiles of peripheral blood mononuclear cells (PBMCs) across Theileria annulata-infected and non-infected crossbred cows. The differential expression profiles from PBMCs of infected and non-infected crossbred cows were generated by RNA sequencing. A marked difference in the expression of genes associated with innate immunity (FTH1, ACTB, ISG15) was observed between the two groups. The over-represented pathways in Theileria annulata-infected cows were associated with the immune system and regulation of the mitotic cycle. Enriched genes and pathways in non-infected animals were associated with the maintenance of chromatin integrity and cell structure. The highly connected genes identified in this study form potential candidates for further investigation into host–parasite interactions in cattle. An improved understanding of the transcriptomic dynamics during theileriosis would lead to underpinning molecular level differences related to the health status of cattle. |
format | Online Article Text |
id | pubmed-10530335 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105303352023-09-28 Exploring the Transcriptome Dynamics of In Vivo Theileria annulata Infection in Crossbred Cattle Ahlawat, Sonika Choudhary, Vikas Arora, Reena Kumar, Ashish Kaur, Mandeep Chhabra, Pooja Genes (Basel) Article The molecular changes occurring in the host in response to in vivo Theileria annulata parasitic infection are not well understood. Therefore, the present study investigated the differential expression profiles of peripheral blood mononuclear cells (PBMCs) across Theileria annulata-infected and non-infected crossbred cows. The differential expression profiles from PBMCs of infected and non-infected crossbred cows were generated by RNA sequencing. A marked difference in the expression of genes associated with innate immunity (FTH1, ACTB, ISG15) was observed between the two groups. The over-represented pathways in Theileria annulata-infected cows were associated with the immune system and regulation of the mitotic cycle. Enriched genes and pathways in non-infected animals were associated with the maintenance of chromatin integrity and cell structure. The highly connected genes identified in this study form potential candidates for further investigation into host–parasite interactions in cattle. An improved understanding of the transcriptomic dynamics during theileriosis would lead to underpinning molecular level differences related to the health status of cattle. MDPI 2023-08-22 /pmc/articles/PMC10530335/ /pubmed/37761803 http://dx.doi.org/10.3390/genes14091663 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ahlawat, Sonika Choudhary, Vikas Arora, Reena Kumar, Ashish Kaur, Mandeep Chhabra, Pooja Exploring the Transcriptome Dynamics of In Vivo Theileria annulata Infection in Crossbred Cattle |
title | Exploring the Transcriptome Dynamics of In Vivo Theileria annulata Infection in Crossbred Cattle |
title_full | Exploring the Transcriptome Dynamics of In Vivo Theileria annulata Infection in Crossbred Cattle |
title_fullStr | Exploring the Transcriptome Dynamics of In Vivo Theileria annulata Infection in Crossbred Cattle |
title_full_unstemmed | Exploring the Transcriptome Dynamics of In Vivo Theileria annulata Infection in Crossbred Cattle |
title_short | Exploring the Transcriptome Dynamics of In Vivo Theileria annulata Infection in Crossbred Cattle |
title_sort | exploring the transcriptome dynamics of in vivo theileria annulata infection in crossbred cattle |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10530335/ https://www.ncbi.nlm.nih.gov/pubmed/37761803 http://dx.doi.org/10.3390/genes14091663 |
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