Cargando…
Development of a Sensitive, Easy and High-Throughput Compliant Protocol for Maize and Soybean DNA Extraction and Quantitation Using a Plant-Specific Universal Taqman Minor Groove Binder Probe
We report the optimization of a high-throughput, compliant DNA extraction method that uses standard format 96-well plates and a commercial automated DNA purification system (ABI PRISM(®) 6100 Nucleic Acid PrepStation). The procedure was set up for maize and soybean, the most common GMO crops and the...
Autores principales: | Ambra, Roberto, Marcelli, Marco, D’Orso, Fabio |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10530873/ https://www.ncbi.nlm.nih.gov/pubmed/37761937 http://dx.doi.org/10.3390/genes14091797 |
Ejemplares similares
-
Monkeypox virus detection in rodents using real-time 3′-minor groove binder TaqMan® assays on the Roche LightCycler
por: Kulesh, David A, et al.
Publicado: (2004) -
Minor Groove Binder Distamycin Remodels Chromatin but Inhibits Transcription
por: Majumder, Parijat, et al.
Publicado: (2013) -
An evaluation of Minor Groove Binders as anti-Trypanosoma brucei brucei therapeutics
por: Scott, Fraser J., et al.
Publicado: (2016) -
Synthesis and Evaluation of Novel DNA Minor Groove Binders as Antiamoebic Agents
por: Alniss, Hasan Y., et al.
Publicado: (2022) -
A low-cost TaqMan minor groove binder probe-based one-step RT-qPCR assay for rapid identification of N501Y variants of SARS-CoV-2
por: Chan, Chloe Toi-Mei, et al.
Publicado: (2022)