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Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair

Mechanically processed stromal vascular fraction (mSVF) is a highly interesting cell source for regenerative purposes, including wound healing, and a practical alternative to enzymatically isolated SVF. In the clinical context, SVF benefits from scaffolds that facilitate viability and other cellular...

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Autores principales: Vasella, Mauro, Arnke, Kevin, Dranseikiene, Dalia, Guzzi, Elia, Melega, Francesca, Reid, Gregory, Klein, Holger Jan, Schweizer, Riccardo, Tibbitt, Mark W., Kim, Bong-Sung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10530931/
https://www.ncbi.nlm.nih.gov/pubmed/37762247
http://dx.doi.org/10.3390/ijms241813944
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author Vasella, Mauro
Arnke, Kevin
Dranseikiene, Dalia
Guzzi, Elia
Melega, Francesca
Reid, Gregory
Klein, Holger Jan
Schweizer, Riccardo
Tibbitt, Mark W.
Kim, Bong-Sung
author_facet Vasella, Mauro
Arnke, Kevin
Dranseikiene, Dalia
Guzzi, Elia
Melega, Francesca
Reid, Gregory
Klein, Holger Jan
Schweizer, Riccardo
Tibbitt, Mark W.
Kim, Bong-Sung
author_sort Vasella, Mauro
collection PubMed
description Mechanically processed stromal vascular fraction (mSVF) is a highly interesting cell source for regenerative purposes, including wound healing, and a practical alternative to enzymatically isolated SVF. In the clinical context, SVF benefits from scaffolds that facilitate viability and other cellular properties. In the present work, the feasibility of methacrylated gelatin (GelMA), a stiffness-tunable, light-inducible hydrogel with high biocompatibility is investigated as a scaffold for SVF in an in vitro setting. Lipoaspirates from elective surgical procedures were collected and processed to mSVF and mixed with GelMA precursor solutions. Non-encapsulated mSVF served as a control. Viability was measured over 21 days. Secreted basic fibroblast growth factor (bFGF) levels were measured on days 1, 7 and 21 by ELISA. IHC was performed to detect VEGF-A, perilipin-2, and CD73 expression on days 7 and 21. The impact of GelMA-mSVF on human dermal fibroblasts was measured in a co-culture assay by the same viability assay. The viability of cultured GelMA-mSVF was significantly higher after 21 days (p < 0.01) when compared to mSVF alone. Also, GelMA-mSVF secreted stable levels of bFGF over 21 days. While VEGF-A was primarily expressed on day 21, perilipin-2 and CD73-positive cells were observed on days 7 and 21. Finally, GelMA-mSVF significantly improved fibroblast viability as compared with GelMA alone (p < 0.01). GelMA may be a promising scaffold for mSVF as it maintains cell viability and proliferation with the release of growth factors while facilitating adipogenic differentiation, stromal cell marker expression and fibroblast proliferation.
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spelling pubmed-105309312023-09-28 Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair Vasella, Mauro Arnke, Kevin Dranseikiene, Dalia Guzzi, Elia Melega, Francesca Reid, Gregory Klein, Holger Jan Schweizer, Riccardo Tibbitt, Mark W. Kim, Bong-Sung Int J Mol Sci Article Mechanically processed stromal vascular fraction (mSVF) is a highly interesting cell source for regenerative purposes, including wound healing, and a practical alternative to enzymatically isolated SVF. In the clinical context, SVF benefits from scaffolds that facilitate viability and other cellular properties. In the present work, the feasibility of methacrylated gelatin (GelMA), a stiffness-tunable, light-inducible hydrogel with high biocompatibility is investigated as a scaffold for SVF in an in vitro setting. Lipoaspirates from elective surgical procedures were collected and processed to mSVF and mixed with GelMA precursor solutions. Non-encapsulated mSVF served as a control. Viability was measured over 21 days. Secreted basic fibroblast growth factor (bFGF) levels were measured on days 1, 7 and 21 by ELISA. IHC was performed to detect VEGF-A, perilipin-2, and CD73 expression on days 7 and 21. The impact of GelMA-mSVF on human dermal fibroblasts was measured in a co-culture assay by the same viability assay. The viability of cultured GelMA-mSVF was significantly higher after 21 days (p < 0.01) when compared to mSVF alone. Also, GelMA-mSVF secreted stable levels of bFGF over 21 days. While VEGF-A was primarily expressed on day 21, perilipin-2 and CD73-positive cells were observed on days 7 and 21. Finally, GelMA-mSVF significantly improved fibroblast viability as compared with GelMA alone (p < 0.01). GelMA may be a promising scaffold for mSVF as it maintains cell viability and proliferation with the release of growth factors while facilitating adipogenic differentiation, stromal cell marker expression and fibroblast proliferation. MDPI 2023-09-11 /pmc/articles/PMC10530931/ /pubmed/37762247 http://dx.doi.org/10.3390/ijms241813944 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vasella, Mauro
Arnke, Kevin
Dranseikiene, Dalia
Guzzi, Elia
Melega, Francesca
Reid, Gregory
Klein, Holger Jan
Schweizer, Riccardo
Tibbitt, Mark W.
Kim, Bong-Sung
Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair
title Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair
title_full Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair
title_fullStr Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair
title_full_unstemmed Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair
title_short Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair
title_sort methacrylated gelatin as a scaffold for mechanically isolated stromal vascular fraction for cutaneous wound repair
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10530931/
https://www.ncbi.nlm.nih.gov/pubmed/37762247
http://dx.doi.org/10.3390/ijms241813944
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