Cargando…
Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair
Mechanically processed stromal vascular fraction (mSVF) is a highly interesting cell source for regenerative purposes, including wound healing, and a practical alternative to enzymatically isolated SVF. In the clinical context, SVF benefits from scaffolds that facilitate viability and other cellular...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10530931/ https://www.ncbi.nlm.nih.gov/pubmed/37762247 http://dx.doi.org/10.3390/ijms241813944 |
_version_ | 1785111601366433792 |
---|---|
author | Vasella, Mauro Arnke, Kevin Dranseikiene, Dalia Guzzi, Elia Melega, Francesca Reid, Gregory Klein, Holger Jan Schweizer, Riccardo Tibbitt, Mark W. Kim, Bong-Sung |
author_facet | Vasella, Mauro Arnke, Kevin Dranseikiene, Dalia Guzzi, Elia Melega, Francesca Reid, Gregory Klein, Holger Jan Schweizer, Riccardo Tibbitt, Mark W. Kim, Bong-Sung |
author_sort | Vasella, Mauro |
collection | PubMed |
description | Mechanically processed stromal vascular fraction (mSVF) is a highly interesting cell source for regenerative purposes, including wound healing, and a practical alternative to enzymatically isolated SVF. In the clinical context, SVF benefits from scaffolds that facilitate viability and other cellular properties. In the present work, the feasibility of methacrylated gelatin (GelMA), a stiffness-tunable, light-inducible hydrogel with high biocompatibility is investigated as a scaffold for SVF in an in vitro setting. Lipoaspirates from elective surgical procedures were collected and processed to mSVF and mixed with GelMA precursor solutions. Non-encapsulated mSVF served as a control. Viability was measured over 21 days. Secreted basic fibroblast growth factor (bFGF) levels were measured on days 1, 7 and 21 by ELISA. IHC was performed to detect VEGF-A, perilipin-2, and CD73 expression on days 7 and 21. The impact of GelMA-mSVF on human dermal fibroblasts was measured in a co-culture assay by the same viability assay. The viability of cultured GelMA-mSVF was significantly higher after 21 days (p < 0.01) when compared to mSVF alone. Also, GelMA-mSVF secreted stable levels of bFGF over 21 days. While VEGF-A was primarily expressed on day 21, perilipin-2 and CD73-positive cells were observed on days 7 and 21. Finally, GelMA-mSVF significantly improved fibroblast viability as compared with GelMA alone (p < 0.01). GelMA may be a promising scaffold for mSVF as it maintains cell viability and proliferation with the release of growth factors while facilitating adipogenic differentiation, stromal cell marker expression and fibroblast proliferation. |
format | Online Article Text |
id | pubmed-10530931 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105309312023-09-28 Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair Vasella, Mauro Arnke, Kevin Dranseikiene, Dalia Guzzi, Elia Melega, Francesca Reid, Gregory Klein, Holger Jan Schweizer, Riccardo Tibbitt, Mark W. Kim, Bong-Sung Int J Mol Sci Article Mechanically processed stromal vascular fraction (mSVF) is a highly interesting cell source for regenerative purposes, including wound healing, and a practical alternative to enzymatically isolated SVF. In the clinical context, SVF benefits from scaffolds that facilitate viability and other cellular properties. In the present work, the feasibility of methacrylated gelatin (GelMA), a stiffness-tunable, light-inducible hydrogel with high biocompatibility is investigated as a scaffold for SVF in an in vitro setting. Lipoaspirates from elective surgical procedures were collected and processed to mSVF and mixed with GelMA precursor solutions. Non-encapsulated mSVF served as a control. Viability was measured over 21 days. Secreted basic fibroblast growth factor (bFGF) levels were measured on days 1, 7 and 21 by ELISA. IHC was performed to detect VEGF-A, perilipin-2, and CD73 expression on days 7 and 21. The impact of GelMA-mSVF on human dermal fibroblasts was measured in a co-culture assay by the same viability assay. The viability of cultured GelMA-mSVF was significantly higher after 21 days (p < 0.01) when compared to mSVF alone. Also, GelMA-mSVF secreted stable levels of bFGF over 21 days. While VEGF-A was primarily expressed on day 21, perilipin-2 and CD73-positive cells were observed on days 7 and 21. Finally, GelMA-mSVF significantly improved fibroblast viability as compared with GelMA alone (p < 0.01). GelMA may be a promising scaffold for mSVF as it maintains cell viability and proliferation with the release of growth factors while facilitating adipogenic differentiation, stromal cell marker expression and fibroblast proliferation. MDPI 2023-09-11 /pmc/articles/PMC10530931/ /pubmed/37762247 http://dx.doi.org/10.3390/ijms241813944 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Vasella, Mauro Arnke, Kevin Dranseikiene, Dalia Guzzi, Elia Melega, Francesca Reid, Gregory Klein, Holger Jan Schweizer, Riccardo Tibbitt, Mark W. Kim, Bong-Sung Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair |
title | Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair |
title_full | Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair |
title_fullStr | Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair |
title_full_unstemmed | Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair |
title_short | Methacrylated Gelatin as a Scaffold for Mechanically Isolated Stromal Vascular Fraction for Cutaneous Wound Repair |
title_sort | methacrylated gelatin as a scaffold for mechanically isolated stromal vascular fraction for cutaneous wound repair |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10530931/ https://www.ncbi.nlm.nih.gov/pubmed/37762247 http://dx.doi.org/10.3390/ijms241813944 |
work_keys_str_mv | AT vasellamauro methacrylatedgelatinasascaffoldformechanicallyisolatedstromalvascularfractionforcutaneouswoundrepair AT arnkekevin methacrylatedgelatinasascaffoldformechanicallyisolatedstromalvascularfractionforcutaneouswoundrepair AT dranseikienedalia methacrylatedgelatinasascaffoldformechanicallyisolatedstromalvascularfractionforcutaneouswoundrepair AT guzzielia methacrylatedgelatinasascaffoldformechanicallyisolatedstromalvascularfractionforcutaneouswoundrepair AT melegafrancesca methacrylatedgelatinasascaffoldformechanicallyisolatedstromalvascularfractionforcutaneouswoundrepair AT reidgregory methacrylatedgelatinasascaffoldformechanicallyisolatedstromalvascularfractionforcutaneouswoundrepair AT kleinholgerjan methacrylatedgelatinasascaffoldformechanicallyisolatedstromalvascularfractionforcutaneouswoundrepair AT schweizerriccardo methacrylatedgelatinasascaffoldformechanicallyisolatedstromalvascularfractionforcutaneouswoundrepair AT tibbittmarkw methacrylatedgelatinasascaffoldformechanicallyisolatedstromalvascularfractionforcutaneouswoundrepair AT kimbongsung methacrylatedgelatinasascaffoldformechanicallyisolatedstromalvascularfractionforcutaneouswoundrepair |