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Evaluation of In Vitro Antioxidant, Anti-Obesity, and Anti-Diabetic Activities of Opuntia ficus Cladodes Gel and Its Application as a Preservative Coating for Shrimp during Refrigerated Storage
Opuntia ficus cladodes (OFC) are considered one of the wastes that result from opuntia cultivation, and their disposal by traditional methods results in many environmental problems. Therefore, this study was conducted with two aims. The first was the production of OFC gel, and the evaluation of its...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10530947/ https://www.ncbi.nlm.nih.gov/pubmed/37754397 http://dx.doi.org/10.3390/gels9090716 |
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author | Mohamed, Alaa S. Elsebaie, Essam Mohamed Abdelrhman, Wesam Mohammed Abdulmaguid, Nabila Yahia Mahmoud Bahnasy, Rasha M. Elgendy, Manal Salah Abbas Elashry, Arwa Mohamed Mohamed Mahmoud El-Hassanin, Marwa Fawzy El-Wakeil, Nora Hamdy Mouhamed Khalil, Azhar Mostafa Mohamed Amin, Hesham F. |
author_facet | Mohamed, Alaa S. Elsebaie, Essam Mohamed Abdelrhman, Wesam Mohammed Abdulmaguid, Nabila Yahia Mahmoud Bahnasy, Rasha M. Elgendy, Manal Salah Abbas Elashry, Arwa Mohamed Mohamed Mahmoud El-Hassanin, Marwa Fawzy El-Wakeil, Nora Hamdy Mouhamed Khalil, Azhar Mostafa Mohamed Amin, Hesham F. |
author_sort | Mohamed, Alaa S. |
collection | PubMed |
description | Opuntia ficus cladodes (OFC) are considered one of the wastes that result from opuntia cultivation, and their disposal by traditional methods results in many environmental problems. Therefore, this study was conducted with two aims. The first was the production of OFC gel, and the evaluation of its in vitro antioxidant (by two methods, DPPH and ABTS), anti-obesity, and anti-diabetic activities. The second was an investigation of the effects of different concentrations of this gel (0, 50, and 100%) as an edible coating on the quality of shrimp during 8 days of refrigerated storage. The results showed that this gel was characterised by a high content of ash (10.42%), total carbohydrates (75.17%), and total phenols (19.79 mg GAE/g). OFC gel contained six types of sugars: arabinose, xylose, galactose, rhamnose, glucose, and uronic acid, and the most abundant was xylose (36.72%). It is also clear from the results that the OFC gel had high antioxidant properties, which were higher against DPPH than ABTS at the same concentration. OFC gel showed a high inhibition activity against lipase, α-glycosidase, and α-amylase enzymes, and their IC(50) values were 1.43 mg/mL, 0.78 mg/mL, and 0.57 mg/mL, respectively. The results also stated that shrimp coated with OFC gel had lower pH, drip loss, TVB-N, and TBA values through the days of refrigerated storage. Moreover, the shrimp coated with 100% OFC gel were better than those coated with 50% OFC gel. In conclusion, OFC gel showed high potency as active antioxidant, for its enzyme anti-activities, and as an edible coating for shrimp. |
format | Online Article Text |
id | pubmed-10530947 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105309472023-09-28 Evaluation of In Vitro Antioxidant, Anti-Obesity, and Anti-Diabetic Activities of Opuntia ficus Cladodes Gel and Its Application as a Preservative Coating for Shrimp during Refrigerated Storage Mohamed, Alaa S. Elsebaie, Essam Mohamed Abdelrhman, Wesam Mohammed Abdulmaguid, Nabila Yahia Mahmoud Bahnasy, Rasha M. Elgendy, Manal Salah Abbas Elashry, Arwa Mohamed Mohamed Mahmoud El-Hassanin, Marwa Fawzy El-Wakeil, Nora Hamdy Mouhamed Khalil, Azhar Mostafa Mohamed Amin, Hesham F. Gels Article Opuntia ficus cladodes (OFC) are considered one of the wastes that result from opuntia cultivation, and their disposal by traditional methods results in many environmental problems. Therefore, this study was conducted with two aims. The first was the production of OFC gel, and the evaluation of its in vitro antioxidant (by two methods, DPPH and ABTS), anti-obesity, and anti-diabetic activities. The second was an investigation of the effects of different concentrations of this gel (0, 50, and 100%) as an edible coating on the quality of shrimp during 8 days of refrigerated storage. The results showed that this gel was characterised by a high content of ash (10.42%), total carbohydrates (75.17%), and total phenols (19.79 mg GAE/g). OFC gel contained six types of sugars: arabinose, xylose, galactose, rhamnose, glucose, and uronic acid, and the most abundant was xylose (36.72%). It is also clear from the results that the OFC gel had high antioxidant properties, which were higher against DPPH than ABTS at the same concentration. OFC gel showed a high inhibition activity against lipase, α-glycosidase, and α-amylase enzymes, and their IC(50) values were 1.43 mg/mL, 0.78 mg/mL, and 0.57 mg/mL, respectively. The results also stated that shrimp coated with OFC gel had lower pH, drip loss, TVB-N, and TBA values through the days of refrigerated storage. Moreover, the shrimp coated with 100% OFC gel were better than those coated with 50% OFC gel. In conclusion, OFC gel showed high potency as active antioxidant, for its enzyme anti-activities, and as an edible coating for shrimp. MDPI 2023-09-04 /pmc/articles/PMC10530947/ /pubmed/37754397 http://dx.doi.org/10.3390/gels9090716 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mohamed, Alaa S. Elsebaie, Essam Mohamed Abdelrhman, Wesam Mohammed Abdulmaguid, Nabila Yahia Mahmoud Bahnasy, Rasha M. Elgendy, Manal Salah Abbas Elashry, Arwa Mohamed Mohamed Mahmoud El-Hassanin, Marwa Fawzy El-Wakeil, Nora Hamdy Mouhamed Khalil, Azhar Mostafa Mohamed Amin, Hesham F. Evaluation of In Vitro Antioxidant, Anti-Obesity, and Anti-Diabetic Activities of Opuntia ficus Cladodes Gel and Its Application as a Preservative Coating for Shrimp during Refrigerated Storage |
title | Evaluation of In Vitro Antioxidant, Anti-Obesity, and Anti-Diabetic Activities of Opuntia ficus Cladodes Gel and Its Application as a Preservative Coating for Shrimp during Refrigerated Storage |
title_full | Evaluation of In Vitro Antioxidant, Anti-Obesity, and Anti-Diabetic Activities of Opuntia ficus Cladodes Gel and Its Application as a Preservative Coating for Shrimp during Refrigerated Storage |
title_fullStr | Evaluation of In Vitro Antioxidant, Anti-Obesity, and Anti-Diabetic Activities of Opuntia ficus Cladodes Gel and Its Application as a Preservative Coating for Shrimp during Refrigerated Storage |
title_full_unstemmed | Evaluation of In Vitro Antioxidant, Anti-Obesity, and Anti-Diabetic Activities of Opuntia ficus Cladodes Gel and Its Application as a Preservative Coating for Shrimp during Refrigerated Storage |
title_short | Evaluation of In Vitro Antioxidant, Anti-Obesity, and Anti-Diabetic Activities of Opuntia ficus Cladodes Gel and Its Application as a Preservative Coating for Shrimp during Refrigerated Storage |
title_sort | evaluation of in vitro antioxidant, anti-obesity, and anti-diabetic activities of opuntia ficus cladodes gel and its application as a preservative coating for shrimp during refrigerated storage |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10530947/ https://www.ncbi.nlm.nih.gov/pubmed/37754397 http://dx.doi.org/10.3390/gels9090716 |
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