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Investigation of Crosslinking Parameters and Characterization of Hyaluronic Acid Dermal Fillers: From Design to Product Performances

Despite process similarities, distinctive manufacturing technologies offer hyaluronic acid dermal fillers with different in vitro physicochemical and rheological properties due to peculiar crosslinked hydrogel networks. A better understanding of dermal filler properties could provide specific clinic...

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Detalles Bibliográficos
Autores principales: Pluda, Stefano, Salvagnini, Cecilia, Fontana, Anna, Marchetti, Anna, Di Lucia, Alba, Galesso, Devis, Guarise, Cristian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10530960/
https://www.ncbi.nlm.nih.gov/pubmed/37754414
http://dx.doi.org/10.3390/gels9090733
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author Pluda, Stefano
Salvagnini, Cecilia
Fontana, Anna
Marchetti, Anna
Di Lucia, Alba
Galesso, Devis
Guarise, Cristian
author_facet Pluda, Stefano
Salvagnini, Cecilia
Fontana, Anna
Marchetti, Anna
Di Lucia, Alba
Galesso, Devis
Guarise, Cristian
author_sort Pluda, Stefano
collection PubMed
description Despite process similarities, distinctive manufacturing technologies offer hyaluronic acid dermal fillers with different in vitro physicochemical and rheological properties due to peculiar crosslinked hydrogel networks. A better understanding of dermal filler properties could provide specific clinical indications and expectations with more accurate performance correlations. In this study, with an emphasis on the degree of modification, hyaluronic acid concentration and molecular weight, these process parameters were able to modulate dermal filler properties, especially rheology. Moreover, an extensive characterization of commercial hyaluronic acid injectables of the Hyal System line was described to present product properties and help to elucidate related clinical effects. Standardized methodologies were applied to correlate in vitro parameters with feasible clinical indications. In view of an optimized dermal filler design, the results of the extrudability measurements allowed the quantification of the effect of hydrogel composition, rheological properties and needle size on injectability. Composition, dynamic viscosity and needle size showed an impactful influence on hydrogel extrudability. Finally, the positive influence of 200 KDa hyaluronic acid in comparison to fragments of ether-crosslinked hyaluronic acid on fibroblast recognition were shown with a migration assay.
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spelling pubmed-105309602023-09-28 Investigation of Crosslinking Parameters and Characterization of Hyaluronic Acid Dermal Fillers: From Design to Product Performances Pluda, Stefano Salvagnini, Cecilia Fontana, Anna Marchetti, Anna Di Lucia, Alba Galesso, Devis Guarise, Cristian Gels Article Despite process similarities, distinctive manufacturing technologies offer hyaluronic acid dermal fillers with different in vitro physicochemical and rheological properties due to peculiar crosslinked hydrogel networks. A better understanding of dermal filler properties could provide specific clinical indications and expectations with more accurate performance correlations. In this study, with an emphasis on the degree of modification, hyaluronic acid concentration and molecular weight, these process parameters were able to modulate dermal filler properties, especially rheology. Moreover, an extensive characterization of commercial hyaluronic acid injectables of the Hyal System line was described to present product properties and help to elucidate related clinical effects. Standardized methodologies were applied to correlate in vitro parameters with feasible clinical indications. In view of an optimized dermal filler design, the results of the extrudability measurements allowed the quantification of the effect of hydrogel composition, rheological properties and needle size on injectability. Composition, dynamic viscosity and needle size showed an impactful influence on hydrogel extrudability. Finally, the positive influence of 200 KDa hyaluronic acid in comparison to fragments of ether-crosslinked hyaluronic acid on fibroblast recognition were shown with a migration assay. MDPI 2023-09-09 /pmc/articles/PMC10530960/ /pubmed/37754414 http://dx.doi.org/10.3390/gels9090733 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pluda, Stefano
Salvagnini, Cecilia
Fontana, Anna
Marchetti, Anna
Di Lucia, Alba
Galesso, Devis
Guarise, Cristian
Investigation of Crosslinking Parameters and Characterization of Hyaluronic Acid Dermal Fillers: From Design to Product Performances
title Investigation of Crosslinking Parameters and Characterization of Hyaluronic Acid Dermal Fillers: From Design to Product Performances
title_full Investigation of Crosslinking Parameters and Characterization of Hyaluronic Acid Dermal Fillers: From Design to Product Performances
title_fullStr Investigation of Crosslinking Parameters and Characterization of Hyaluronic Acid Dermal Fillers: From Design to Product Performances
title_full_unstemmed Investigation of Crosslinking Parameters and Characterization of Hyaluronic Acid Dermal Fillers: From Design to Product Performances
title_short Investigation of Crosslinking Parameters and Characterization of Hyaluronic Acid Dermal Fillers: From Design to Product Performances
title_sort investigation of crosslinking parameters and characterization of hyaluronic acid dermal fillers: from design to product performances
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10530960/
https://www.ncbi.nlm.nih.gov/pubmed/37754414
http://dx.doi.org/10.3390/gels9090733
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