Cargando…

Genomic and Transcriptomic Insights into the Evolution and Divergence of MIKC-Type MADS-Box Genes in Carica papaya

MIKC-type MADS-box genes, also known as type II genes, play a crucial role in regulating the formation of floral organs and reproductive development in plants. However, the genome-wide identification and characterization of type II genes as well as a transcriptomic survey of their potential roles in...

Descripción completa

Detalles Bibliográficos
Autores principales: Dai, Yunsu, Wang, Yu, Zeng, Liwang, Jia, Ruizong, He, Linwen, Huang, Xueying, Zhao, Hui, Liu, Difa, Zhao, Haixu, Hu, Shuai, Gao, Ling, Guo, Anping, Xia, Wei, Ji, Changmian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531014/
https://www.ncbi.nlm.nih.gov/pubmed/37762345
http://dx.doi.org/10.3390/ijms241814039
_version_ 1785111621421498368
author Dai, Yunsu
Wang, Yu
Zeng, Liwang
Jia, Ruizong
He, Linwen
Huang, Xueying
Zhao, Hui
Liu, Difa
Zhao, Haixu
Hu, Shuai
Gao, Ling
Guo, Anping
Xia, Wei
Ji, Changmian
author_facet Dai, Yunsu
Wang, Yu
Zeng, Liwang
Jia, Ruizong
He, Linwen
Huang, Xueying
Zhao, Hui
Liu, Difa
Zhao, Haixu
Hu, Shuai
Gao, Ling
Guo, Anping
Xia, Wei
Ji, Changmian
author_sort Dai, Yunsu
collection PubMed
description MIKC-type MADS-box genes, also known as type II genes, play a crucial role in regulating the formation of floral organs and reproductive development in plants. However, the genome-wide identification and characterization of type II genes as well as a transcriptomic survey of their potential roles in Carica papaya remain unresolved. Here, we identified and characterized 24 type II genes in the C. papaya genome, and investigated their evolutional scenario and potential roles with a widespread expression profile. The type II genes were divided into thirteen subclades, and gene loss events likely occurred in papaya, as evidenced by the contracted member size of most subclades. Gene duplication mainly contributed to MIKC-type gene formation in papaya, and the duplicated gene pairs displayed prevalent expression divergence, implying the evolutionary significance of gene duplication in shaping the diversity of type II genes in papaya. A large-scale transcriptome analysis of 152 samples indicated that different subclasses of these genes showed distinct expression patterns in various tissues, biotic stress response, and abiotic stress response, reflecting their divergent functions. The hub-network of male and female flowers and qRT-PCR suggested that TT16-3 and AGL8 participated in male flower development and seed germination. Overall, this study provides valuable insights into the evolution and functions of MIKC-type genes in C. papaya.
format Online
Article
Text
id pubmed-10531014
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-105310142023-09-28 Genomic and Transcriptomic Insights into the Evolution and Divergence of MIKC-Type MADS-Box Genes in Carica papaya Dai, Yunsu Wang, Yu Zeng, Liwang Jia, Ruizong He, Linwen Huang, Xueying Zhao, Hui Liu, Difa Zhao, Haixu Hu, Shuai Gao, Ling Guo, Anping Xia, Wei Ji, Changmian Int J Mol Sci Article MIKC-type MADS-box genes, also known as type II genes, play a crucial role in regulating the formation of floral organs and reproductive development in plants. However, the genome-wide identification and characterization of type II genes as well as a transcriptomic survey of their potential roles in Carica papaya remain unresolved. Here, we identified and characterized 24 type II genes in the C. papaya genome, and investigated their evolutional scenario and potential roles with a widespread expression profile. The type II genes were divided into thirteen subclades, and gene loss events likely occurred in papaya, as evidenced by the contracted member size of most subclades. Gene duplication mainly contributed to MIKC-type gene formation in papaya, and the duplicated gene pairs displayed prevalent expression divergence, implying the evolutionary significance of gene duplication in shaping the diversity of type II genes in papaya. A large-scale transcriptome analysis of 152 samples indicated that different subclasses of these genes showed distinct expression patterns in various tissues, biotic stress response, and abiotic stress response, reflecting their divergent functions. The hub-network of male and female flowers and qRT-PCR suggested that TT16-3 and AGL8 participated in male flower development and seed germination. Overall, this study provides valuable insights into the evolution and functions of MIKC-type genes in C. papaya. MDPI 2023-09-13 /pmc/articles/PMC10531014/ /pubmed/37762345 http://dx.doi.org/10.3390/ijms241814039 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dai, Yunsu
Wang, Yu
Zeng, Liwang
Jia, Ruizong
He, Linwen
Huang, Xueying
Zhao, Hui
Liu, Difa
Zhao, Haixu
Hu, Shuai
Gao, Ling
Guo, Anping
Xia, Wei
Ji, Changmian
Genomic and Transcriptomic Insights into the Evolution and Divergence of MIKC-Type MADS-Box Genes in Carica papaya
title Genomic and Transcriptomic Insights into the Evolution and Divergence of MIKC-Type MADS-Box Genes in Carica papaya
title_full Genomic and Transcriptomic Insights into the Evolution and Divergence of MIKC-Type MADS-Box Genes in Carica papaya
title_fullStr Genomic and Transcriptomic Insights into the Evolution and Divergence of MIKC-Type MADS-Box Genes in Carica papaya
title_full_unstemmed Genomic and Transcriptomic Insights into the Evolution and Divergence of MIKC-Type MADS-Box Genes in Carica papaya
title_short Genomic and Transcriptomic Insights into the Evolution and Divergence of MIKC-Type MADS-Box Genes in Carica papaya
title_sort genomic and transcriptomic insights into the evolution and divergence of mikc-type mads-box genes in carica papaya
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531014/
https://www.ncbi.nlm.nih.gov/pubmed/37762345
http://dx.doi.org/10.3390/ijms241814039
work_keys_str_mv AT daiyunsu genomicandtranscriptomicinsightsintotheevolutionanddivergenceofmikctypemadsboxgenesincaricapapaya
AT wangyu genomicandtranscriptomicinsightsintotheevolutionanddivergenceofmikctypemadsboxgenesincaricapapaya
AT zengliwang genomicandtranscriptomicinsightsintotheevolutionanddivergenceofmikctypemadsboxgenesincaricapapaya
AT jiaruizong genomicandtranscriptomicinsightsintotheevolutionanddivergenceofmikctypemadsboxgenesincaricapapaya
AT helinwen genomicandtranscriptomicinsightsintotheevolutionanddivergenceofmikctypemadsboxgenesincaricapapaya
AT huangxueying genomicandtranscriptomicinsightsintotheevolutionanddivergenceofmikctypemadsboxgenesincaricapapaya
AT zhaohui genomicandtranscriptomicinsightsintotheevolutionanddivergenceofmikctypemadsboxgenesincaricapapaya
AT liudifa genomicandtranscriptomicinsightsintotheevolutionanddivergenceofmikctypemadsboxgenesincaricapapaya
AT zhaohaixu genomicandtranscriptomicinsightsintotheevolutionanddivergenceofmikctypemadsboxgenesincaricapapaya
AT hushuai genomicandtranscriptomicinsightsintotheevolutionanddivergenceofmikctypemadsboxgenesincaricapapaya
AT gaoling genomicandtranscriptomicinsightsintotheevolutionanddivergenceofmikctypemadsboxgenesincaricapapaya
AT guoanping genomicandtranscriptomicinsightsintotheevolutionanddivergenceofmikctypemadsboxgenesincaricapapaya
AT xiawei genomicandtranscriptomicinsightsintotheevolutionanddivergenceofmikctypemadsboxgenesincaricapapaya
AT jichangmian genomicandtranscriptomicinsightsintotheevolutionanddivergenceofmikctypemadsboxgenesincaricapapaya