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Feasibility of Enzymatic Protein Extraction from a Dehydrated Fish Biomass Obtained from Unsorted Canned Yellowfin Tuna Side Streams: Part I

This study presents for the first time a scalable process for the extraction of valuable proteins starting from samples of unsorted mixed tuna scraps which were previously dehydrated by an industrial patented process. The aims of this work were both to avoid the onerous sorting step of tuna leftover...

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Autores principales: Grasso, Federica, Méndez-Paz, Diego, Vázquez Sobrado, Rebeca, Orlandi, Valentina, Turrini, Federica, De Negri Atanasio, Giulia, Grasselli, Elena, Tiso, Micaela, Boggia, Raffaella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531079/
https://www.ncbi.nlm.nih.gov/pubmed/37754441
http://dx.doi.org/10.3390/gels9090760
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author Grasso, Federica
Méndez-Paz, Diego
Vázquez Sobrado, Rebeca
Orlandi, Valentina
Turrini, Federica
De Negri Atanasio, Giulia
Grasselli, Elena
Tiso, Micaela
Boggia, Raffaella
author_facet Grasso, Federica
Méndez-Paz, Diego
Vázquez Sobrado, Rebeca
Orlandi, Valentina
Turrini, Federica
De Negri Atanasio, Giulia
Grasselli, Elena
Tiso, Micaela
Boggia, Raffaella
author_sort Grasso, Federica
collection PubMed
description This study presents for the first time a scalable process for the extraction of valuable proteins starting from samples of unsorted mixed tuna scraps which were previously dehydrated by an industrial patented process. The aims of this work were both to avoid the onerous sorting step of tuna leftovers, which generally consists of isolating skin and bones for collagen/gelatin extraction, and to improve the logistic of managing highly perishable biomass thanks to the reduction in its volume and to its microbiological stabilization. In view of a zero-waste economy, all the protein fractions (namely, non-collagenous proteins NCs and ALKs, gelatin, and hydrolyzed gelatin peptides, HGPs) isolated in the proposed single cascade flowchart were stabilized and preliminarily characterized. The extraction flowchart proposed allows one to obtain the following most promising compounds: 1.7 g of gelatin, 3.2 g of HGPs, and 14.6 g of NCs per 100 g of dehydrated starting material. A focus on oven-dried gelatin was reported in terms of proximate analysis, amino acid composition, color parameters, FT-IR spectrum, pH, and viscoelastic properties (5 mPa·s of viscosity and 14.3 °C of gelling temperature). All the obtained extracts are intended to be exploited in food supplements, feed, fertilizers/plant bio-stimulants, packaging, and the cosmetic industry.
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spelling pubmed-105310792023-09-28 Feasibility of Enzymatic Protein Extraction from a Dehydrated Fish Biomass Obtained from Unsorted Canned Yellowfin Tuna Side Streams: Part I Grasso, Federica Méndez-Paz, Diego Vázquez Sobrado, Rebeca Orlandi, Valentina Turrini, Federica De Negri Atanasio, Giulia Grasselli, Elena Tiso, Micaela Boggia, Raffaella Gels Article This study presents for the first time a scalable process for the extraction of valuable proteins starting from samples of unsorted mixed tuna scraps which were previously dehydrated by an industrial patented process. The aims of this work were both to avoid the onerous sorting step of tuna leftovers, which generally consists of isolating skin and bones for collagen/gelatin extraction, and to improve the logistic of managing highly perishable biomass thanks to the reduction in its volume and to its microbiological stabilization. In view of a zero-waste economy, all the protein fractions (namely, non-collagenous proteins NCs and ALKs, gelatin, and hydrolyzed gelatin peptides, HGPs) isolated in the proposed single cascade flowchart were stabilized and preliminarily characterized. The extraction flowchart proposed allows one to obtain the following most promising compounds: 1.7 g of gelatin, 3.2 g of HGPs, and 14.6 g of NCs per 100 g of dehydrated starting material. A focus on oven-dried gelatin was reported in terms of proximate analysis, amino acid composition, color parameters, FT-IR spectrum, pH, and viscoelastic properties (5 mPa·s of viscosity and 14.3 °C of gelling temperature). All the obtained extracts are intended to be exploited in food supplements, feed, fertilizers/plant bio-stimulants, packaging, and the cosmetic industry. MDPI 2023-09-18 /pmc/articles/PMC10531079/ /pubmed/37754441 http://dx.doi.org/10.3390/gels9090760 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grasso, Federica
Méndez-Paz, Diego
Vázquez Sobrado, Rebeca
Orlandi, Valentina
Turrini, Federica
De Negri Atanasio, Giulia
Grasselli, Elena
Tiso, Micaela
Boggia, Raffaella
Feasibility of Enzymatic Protein Extraction from a Dehydrated Fish Biomass Obtained from Unsorted Canned Yellowfin Tuna Side Streams: Part I
title Feasibility of Enzymatic Protein Extraction from a Dehydrated Fish Biomass Obtained from Unsorted Canned Yellowfin Tuna Side Streams: Part I
title_full Feasibility of Enzymatic Protein Extraction from a Dehydrated Fish Biomass Obtained from Unsorted Canned Yellowfin Tuna Side Streams: Part I
title_fullStr Feasibility of Enzymatic Protein Extraction from a Dehydrated Fish Biomass Obtained from Unsorted Canned Yellowfin Tuna Side Streams: Part I
title_full_unstemmed Feasibility of Enzymatic Protein Extraction from a Dehydrated Fish Biomass Obtained from Unsorted Canned Yellowfin Tuna Side Streams: Part I
title_short Feasibility of Enzymatic Protein Extraction from a Dehydrated Fish Biomass Obtained from Unsorted Canned Yellowfin Tuna Side Streams: Part I
title_sort feasibility of enzymatic protein extraction from a dehydrated fish biomass obtained from unsorted canned yellowfin tuna side streams: part i
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531079/
https://www.ncbi.nlm.nih.gov/pubmed/37754441
http://dx.doi.org/10.3390/gels9090760
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