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Association between the Cytosine Hydroxymethylation and the Expression of microRNA in Multiple Sclerosis in Polish Population

Multiple sclerosis is a chronic demyelinating disorder with an unclear etiology. A key role is thought to be played by Th17 cells and microRNAs associated with Th17, such as miR-155, miR-326 and miR-223. The present study compared the methylation and hydroxymethylation levels of CpG sites within pro...

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Autores principales: Basak, Justyna, Piotrzkowska, Danuta, Kucharska-Lusina, Aleksandra, Majsterek, Ireneusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531266/
https://www.ncbi.nlm.nih.gov/pubmed/37762229
http://dx.doi.org/10.3390/ijms241813923
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author Basak, Justyna
Piotrzkowska, Danuta
Kucharska-Lusina, Aleksandra
Majsterek, Ireneusz
author_facet Basak, Justyna
Piotrzkowska, Danuta
Kucharska-Lusina, Aleksandra
Majsterek, Ireneusz
author_sort Basak, Justyna
collection PubMed
description Multiple sclerosis is a chronic demyelinating disorder with an unclear etiology. A key role is thought to be played by Th17 cells and microRNAs associated with Th17, such as miR-155, miR-326 and miR-223. The present study compared the methylation and hydroxymethylation levels of CpG sites within promoters of these microRNA between MS patients and controls using PBMCs and analyzed their relationship with microRNA expression. Significant intergroup differences were found between the levels of 5-hmC within the CpG-1 miR-155 promoter and CpG within the miR-326 promoter; in addition, miR-155-5p and miR-223-3p expression was elevated in MS patients. Correlation analysis showed a positive relationship between the level of 5-hmC of CpG-2 in the miR-223 promoter and miR-223-3p level. As it is possible to pharmacologically modulate the level of epigenetic modifications, our findings cast light on the etiology of MS and support the development of more effective therapies.
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spelling pubmed-105312662023-09-28 Association between the Cytosine Hydroxymethylation and the Expression of microRNA in Multiple Sclerosis in Polish Population Basak, Justyna Piotrzkowska, Danuta Kucharska-Lusina, Aleksandra Majsterek, Ireneusz Int J Mol Sci Article Multiple sclerosis is a chronic demyelinating disorder with an unclear etiology. A key role is thought to be played by Th17 cells and microRNAs associated with Th17, such as miR-155, miR-326 and miR-223. The present study compared the methylation and hydroxymethylation levels of CpG sites within promoters of these microRNA between MS patients and controls using PBMCs and analyzed their relationship with microRNA expression. Significant intergroup differences were found between the levels of 5-hmC within the CpG-1 miR-155 promoter and CpG within the miR-326 promoter; in addition, miR-155-5p and miR-223-3p expression was elevated in MS patients. Correlation analysis showed a positive relationship between the level of 5-hmC of CpG-2 in the miR-223 promoter and miR-223-3p level. As it is possible to pharmacologically modulate the level of epigenetic modifications, our findings cast light on the etiology of MS and support the development of more effective therapies. MDPI 2023-09-10 /pmc/articles/PMC10531266/ /pubmed/37762229 http://dx.doi.org/10.3390/ijms241813923 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Basak, Justyna
Piotrzkowska, Danuta
Kucharska-Lusina, Aleksandra
Majsterek, Ireneusz
Association between the Cytosine Hydroxymethylation and the Expression of microRNA in Multiple Sclerosis in Polish Population
title Association between the Cytosine Hydroxymethylation and the Expression of microRNA in Multiple Sclerosis in Polish Population
title_full Association between the Cytosine Hydroxymethylation and the Expression of microRNA in Multiple Sclerosis in Polish Population
title_fullStr Association between the Cytosine Hydroxymethylation and the Expression of microRNA in Multiple Sclerosis in Polish Population
title_full_unstemmed Association between the Cytosine Hydroxymethylation and the Expression of microRNA in Multiple Sclerosis in Polish Population
title_short Association between the Cytosine Hydroxymethylation and the Expression of microRNA in Multiple Sclerosis in Polish Population
title_sort association between the cytosine hydroxymethylation and the expression of microrna in multiple sclerosis in polish population
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531266/
https://www.ncbi.nlm.nih.gov/pubmed/37762229
http://dx.doi.org/10.3390/ijms241813923
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