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The MothersBabies Study, an Australian Prospective Cohort Study Analyzing the Microbiome in the Preconception and Perinatal Period to Determine Risk of Adverse Pregnancy, Postpartum, and Child-Related Health Outcomes: Study Protocol

The microbiome has emerged as a key determinant of human health and reproduction, with recent evidence suggesting a dysbiotic microbiome is implicated in adverse perinatal health outcomes. The existing research has been limited by the sample collection and timing, cohort design, sample design, and l...

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Autores principales: Strout, Naomi, Pasic, Lana, Hicks, Chloe, Chua, Xin-Yi, Tashvighi, Niki, Butler, Phoebe, Liu, Zhixin, El-Assaad, Fatima, Holmes, Elaine, Susic, Daniella, Samaras, Katherine, Craig, Maria E., Davis, Gregory K., Henry, Amanda, Ledger, William L., El-Omar, Emad M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531411/
https://www.ncbi.nlm.nih.gov/pubmed/37754596
http://dx.doi.org/10.3390/ijerph20186736
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author Strout, Naomi
Pasic, Lana
Hicks, Chloe
Chua, Xin-Yi
Tashvighi, Niki
Butler, Phoebe
Liu, Zhixin
El-Assaad, Fatima
Holmes, Elaine
Susic, Daniella
Samaras, Katherine
Craig, Maria E.
Davis, Gregory K.
Henry, Amanda
Ledger, William L.
El-Omar, Emad M.
author_facet Strout, Naomi
Pasic, Lana
Hicks, Chloe
Chua, Xin-Yi
Tashvighi, Niki
Butler, Phoebe
Liu, Zhixin
El-Assaad, Fatima
Holmes, Elaine
Susic, Daniella
Samaras, Katherine
Craig, Maria E.
Davis, Gregory K.
Henry, Amanda
Ledger, William L.
El-Omar, Emad M.
author_sort Strout, Naomi
collection PubMed
description The microbiome has emerged as a key determinant of human health and reproduction, with recent evidence suggesting a dysbiotic microbiome is implicated in adverse perinatal health outcomes. The existing research has been limited by the sample collection and timing, cohort design, sample design, and lack of data on the preconception microbiome. This prospective, longitudinal cohort study will recruit 2000 Australian women, in order to fully explore the role of the microbiome in the development of adverse perinatal outcomes. Participants are enrolled for a maximum of 7 years, from 1 year preconception, through to 5 years postpartum. Assessment occurs every three months until pregnancy occurs, then during Trimester 1 (5 + 0–12 + 6 weeks gestation), Trimester 2 (20 + 0–24 + 6 weeks gestation), Trimester 3 (32 + 0–36 + 6 weeks gestation), and postpartum at 1 week, 2 months, 6 months, and then annually from 1 to 5 years. At each assessment, maternal participants self-collect oral, skin, vaginal, urine, and stool samples. Oral, skin, urine, and stool samples will be collected from children. Blood samples will be obtained from maternal participants who can access a study collection center. The measurements taken will include anthropometric, blood pressure, heart rate, and serum hormonal and metabolic parameters. Validated self-report questionnaires will be administered to assess diet, physical activity, mental health, and child developmental milestones. Medications, medical, surgical, obstetric history, the impact of COVID-19, living environments, and pregnancy and child health outcomes will be recorded. Multiomic bioinformatic and statistical analyses will assess the association between participants who developed high-risk and low-risk pregnancies, adverse postnatal conditions, and/or childhood disease, and their microbiome for the different sample types.
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spelling pubmed-105314112023-09-28 The MothersBabies Study, an Australian Prospective Cohort Study Analyzing the Microbiome in the Preconception and Perinatal Period to Determine Risk of Adverse Pregnancy, Postpartum, and Child-Related Health Outcomes: Study Protocol Strout, Naomi Pasic, Lana Hicks, Chloe Chua, Xin-Yi Tashvighi, Niki Butler, Phoebe Liu, Zhixin El-Assaad, Fatima Holmes, Elaine Susic, Daniella Samaras, Katherine Craig, Maria E. Davis, Gregory K. Henry, Amanda Ledger, William L. El-Omar, Emad M. Int J Environ Res Public Health Study Protocol The microbiome has emerged as a key determinant of human health and reproduction, with recent evidence suggesting a dysbiotic microbiome is implicated in adverse perinatal health outcomes. The existing research has been limited by the sample collection and timing, cohort design, sample design, and lack of data on the preconception microbiome. This prospective, longitudinal cohort study will recruit 2000 Australian women, in order to fully explore the role of the microbiome in the development of adverse perinatal outcomes. Participants are enrolled for a maximum of 7 years, from 1 year preconception, through to 5 years postpartum. Assessment occurs every three months until pregnancy occurs, then during Trimester 1 (5 + 0–12 + 6 weeks gestation), Trimester 2 (20 + 0–24 + 6 weeks gestation), Trimester 3 (32 + 0–36 + 6 weeks gestation), and postpartum at 1 week, 2 months, 6 months, and then annually from 1 to 5 years. At each assessment, maternal participants self-collect oral, skin, vaginal, urine, and stool samples. Oral, skin, urine, and stool samples will be collected from children. Blood samples will be obtained from maternal participants who can access a study collection center. The measurements taken will include anthropometric, blood pressure, heart rate, and serum hormonal and metabolic parameters. Validated self-report questionnaires will be administered to assess diet, physical activity, mental health, and child developmental milestones. Medications, medical, surgical, obstetric history, the impact of COVID-19, living environments, and pregnancy and child health outcomes will be recorded. Multiomic bioinformatic and statistical analyses will assess the association between participants who developed high-risk and low-risk pregnancies, adverse postnatal conditions, and/or childhood disease, and their microbiome for the different sample types. MDPI 2023-09-09 /pmc/articles/PMC10531411/ /pubmed/37754596 http://dx.doi.org/10.3390/ijerph20186736 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Study Protocol
Strout, Naomi
Pasic, Lana
Hicks, Chloe
Chua, Xin-Yi
Tashvighi, Niki
Butler, Phoebe
Liu, Zhixin
El-Assaad, Fatima
Holmes, Elaine
Susic, Daniella
Samaras, Katherine
Craig, Maria E.
Davis, Gregory K.
Henry, Amanda
Ledger, William L.
El-Omar, Emad M.
The MothersBabies Study, an Australian Prospective Cohort Study Analyzing the Microbiome in the Preconception and Perinatal Period to Determine Risk of Adverse Pregnancy, Postpartum, and Child-Related Health Outcomes: Study Protocol
title The MothersBabies Study, an Australian Prospective Cohort Study Analyzing the Microbiome in the Preconception and Perinatal Period to Determine Risk of Adverse Pregnancy, Postpartum, and Child-Related Health Outcomes: Study Protocol
title_full The MothersBabies Study, an Australian Prospective Cohort Study Analyzing the Microbiome in the Preconception and Perinatal Period to Determine Risk of Adverse Pregnancy, Postpartum, and Child-Related Health Outcomes: Study Protocol
title_fullStr The MothersBabies Study, an Australian Prospective Cohort Study Analyzing the Microbiome in the Preconception and Perinatal Period to Determine Risk of Adverse Pregnancy, Postpartum, and Child-Related Health Outcomes: Study Protocol
title_full_unstemmed The MothersBabies Study, an Australian Prospective Cohort Study Analyzing the Microbiome in the Preconception and Perinatal Period to Determine Risk of Adverse Pregnancy, Postpartum, and Child-Related Health Outcomes: Study Protocol
title_short The MothersBabies Study, an Australian Prospective Cohort Study Analyzing the Microbiome in the Preconception and Perinatal Period to Determine Risk of Adverse Pregnancy, Postpartum, and Child-Related Health Outcomes: Study Protocol
title_sort mothersbabies study, an australian prospective cohort study analyzing the microbiome in the preconception and perinatal period to determine risk of adverse pregnancy, postpartum, and child-related health outcomes: study protocol
topic Study Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531411/
https://www.ncbi.nlm.nih.gov/pubmed/37754596
http://dx.doi.org/10.3390/ijerph20186736
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