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Multi-Omics Analysis Reveals the Pathogenesis of Growth-Disordered Raccoon Dog

Microorganisms of the genus Eperythrozoon are a zoonotic chronic infectious disease with wide distribution. We found that raccoons infected with Eperythrozoon showed obvious stunting, which seriously affected the economic benefits of raccoon dogs. To investigate the pathogenesis of the raccoon dog,...

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Autores principales: Chen, Danyang, Guo, Xiaolan, Wang, Kaiying, Zhao, Weigang, Chang, Zhongjuan, Wang, Quankai, Xu, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531513/
https://www.ncbi.nlm.nih.gov/pubmed/37762538
http://dx.doi.org/10.3390/ijms241814237
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author Chen, Danyang
Guo, Xiaolan
Wang, Kaiying
Zhao, Weigang
Chang, Zhongjuan
Wang, Quankai
Xu, Chao
author_facet Chen, Danyang
Guo, Xiaolan
Wang, Kaiying
Zhao, Weigang
Chang, Zhongjuan
Wang, Quankai
Xu, Chao
author_sort Chen, Danyang
collection PubMed
description Microorganisms of the genus Eperythrozoon are a zoonotic chronic infectious disease with wide distribution. We found that raccoons infected with Eperythrozoon showed obvious stunting, which seriously affected the economic benefits of raccoon dogs. To investigate the pathogenesis of the raccoon dog, we used transcriptome and proteome sequencing to analyze the changes in mRNA, miRNA, and protein expression in raccoon dogs infected with Eperythrozoon and normal raccoons. The results showed that the expression levels of genes related to immunity, metabolism, and enzyme activity were significantly changed. Among these, ERLIN1, IGF1R, CREB3L1, TNS1, TENC1, and mTOR play key roles. Additionally, the miR-1268, miR-125b, miR-10-5p, and miR-10 as central miRNAs regulate the expression of these genes. Integrated transcriptomic and proteomic analyses revealed consistent trends in mRNA and protein changes in MYH9, FKBP1A, PRKCA, and CYP11B2. These results suggest that Eperythrozoon may contribute to the slow development of raccoons by affecting the expression of mRNAs and miRNAs, reducing their immunity and causing metabolic abnormalities.
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spelling pubmed-105315132023-09-28 Multi-Omics Analysis Reveals the Pathogenesis of Growth-Disordered Raccoon Dog Chen, Danyang Guo, Xiaolan Wang, Kaiying Zhao, Weigang Chang, Zhongjuan Wang, Quankai Xu, Chao Int J Mol Sci Article Microorganisms of the genus Eperythrozoon are a zoonotic chronic infectious disease with wide distribution. We found that raccoons infected with Eperythrozoon showed obvious stunting, which seriously affected the economic benefits of raccoon dogs. To investigate the pathogenesis of the raccoon dog, we used transcriptome and proteome sequencing to analyze the changes in mRNA, miRNA, and protein expression in raccoon dogs infected with Eperythrozoon and normal raccoons. The results showed that the expression levels of genes related to immunity, metabolism, and enzyme activity were significantly changed. Among these, ERLIN1, IGF1R, CREB3L1, TNS1, TENC1, and mTOR play key roles. Additionally, the miR-1268, miR-125b, miR-10-5p, and miR-10 as central miRNAs regulate the expression of these genes. Integrated transcriptomic and proteomic analyses revealed consistent trends in mRNA and protein changes in MYH9, FKBP1A, PRKCA, and CYP11B2. These results suggest that Eperythrozoon may contribute to the slow development of raccoons by affecting the expression of mRNAs and miRNAs, reducing their immunity and causing metabolic abnormalities. MDPI 2023-09-18 /pmc/articles/PMC10531513/ /pubmed/37762538 http://dx.doi.org/10.3390/ijms241814237 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Danyang
Guo, Xiaolan
Wang, Kaiying
Zhao, Weigang
Chang, Zhongjuan
Wang, Quankai
Xu, Chao
Multi-Omics Analysis Reveals the Pathogenesis of Growth-Disordered Raccoon Dog
title Multi-Omics Analysis Reveals the Pathogenesis of Growth-Disordered Raccoon Dog
title_full Multi-Omics Analysis Reveals the Pathogenesis of Growth-Disordered Raccoon Dog
title_fullStr Multi-Omics Analysis Reveals the Pathogenesis of Growth-Disordered Raccoon Dog
title_full_unstemmed Multi-Omics Analysis Reveals the Pathogenesis of Growth-Disordered Raccoon Dog
title_short Multi-Omics Analysis Reveals the Pathogenesis of Growth-Disordered Raccoon Dog
title_sort multi-omics analysis reveals the pathogenesis of growth-disordered raccoon dog
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531513/
https://www.ncbi.nlm.nih.gov/pubmed/37762538
http://dx.doi.org/10.3390/ijms241814237
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