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METTL3 Promotes the Differentiation of Goat Skeletal Muscle Satellite Cells by Regulating MEF2C mRNA Stability in a m(6)A-Dependent Manner
The development of mammalian skeletal muscle is a highly complex process involving multiple molecular interactions. As a prevalent RNA modification, N6-methyladenosine (m(6)A) regulates the expression of target genes to affect mammalian development. Nevertheless, it remains unclear how m(6)A partici...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531580/ https://www.ncbi.nlm.nih.gov/pubmed/37762418 http://dx.doi.org/10.3390/ijms241814115 |
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author | Zhao, Sen Cao, Jiaxue Sun, Yanjin Zhou, Helin Zhu, Qi Dai, Dinghui Zhan, Siyuan Guo, Jiazhong Zhong, Tao Wang, Linjie Li, Li Zhang, Hongping |
author_facet | Zhao, Sen Cao, Jiaxue Sun, Yanjin Zhou, Helin Zhu, Qi Dai, Dinghui Zhan, Siyuan Guo, Jiazhong Zhong, Tao Wang, Linjie Li, Li Zhang, Hongping |
author_sort | Zhao, Sen |
collection | PubMed |
description | The development of mammalian skeletal muscle is a highly complex process involving multiple molecular interactions. As a prevalent RNA modification, N6-methyladenosine (m(6)A) regulates the expression of target genes to affect mammalian development. Nevertheless, it remains unclear how m(6)A participates in the development of goat muscle. In this study, methyltransferase 3 (METTL3) was significantly enriched in goat longissimus dorsi (LD) tissue. In addition, the global m(6)A modification level and differentiation of skeletal muscle satellite cells (MuSCs) were regulated by METTL3. By performing mRNA-seq analysis, 8050 candidate genes exhibited significant changes in expression level after the knockdown of METTL3 in MuSCs. Additionally, methylated RNA immunoprecipitation sequencing (MeRIP-seq) illustrated that myocyte enhancer factor 2c (MEF2C) mRNA contained m(6)A modification. Further experiments demonstrated that METTL3 enhanced the differentiation of MuSCs by upregulating m(6)A levels and expression of MEF2C. Moreover, the m(6)A reader YTH N6-methyladenosine RNA binding protein C1 (YTHDC1) was bound and stabilized to MEF2C mRNA. The present study reveals that METTL3 enhances myogenic differentiation in MuSCs by regulating MEF2C and provides evidence of a post-transcriptional mechanism in the development of goat skeletal muscle. |
format | Online Article Text |
id | pubmed-10531580 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105315802023-09-28 METTL3 Promotes the Differentiation of Goat Skeletal Muscle Satellite Cells by Regulating MEF2C mRNA Stability in a m(6)A-Dependent Manner Zhao, Sen Cao, Jiaxue Sun, Yanjin Zhou, Helin Zhu, Qi Dai, Dinghui Zhan, Siyuan Guo, Jiazhong Zhong, Tao Wang, Linjie Li, Li Zhang, Hongping Int J Mol Sci Article The development of mammalian skeletal muscle is a highly complex process involving multiple molecular interactions. As a prevalent RNA modification, N6-methyladenosine (m(6)A) regulates the expression of target genes to affect mammalian development. Nevertheless, it remains unclear how m(6)A participates in the development of goat muscle. In this study, methyltransferase 3 (METTL3) was significantly enriched in goat longissimus dorsi (LD) tissue. In addition, the global m(6)A modification level and differentiation of skeletal muscle satellite cells (MuSCs) were regulated by METTL3. By performing mRNA-seq analysis, 8050 candidate genes exhibited significant changes in expression level after the knockdown of METTL3 in MuSCs. Additionally, methylated RNA immunoprecipitation sequencing (MeRIP-seq) illustrated that myocyte enhancer factor 2c (MEF2C) mRNA contained m(6)A modification. Further experiments demonstrated that METTL3 enhanced the differentiation of MuSCs by upregulating m(6)A levels and expression of MEF2C. Moreover, the m(6)A reader YTH N6-methyladenosine RNA binding protein C1 (YTHDC1) was bound and stabilized to MEF2C mRNA. The present study reveals that METTL3 enhances myogenic differentiation in MuSCs by regulating MEF2C and provides evidence of a post-transcriptional mechanism in the development of goat skeletal muscle. MDPI 2023-09-14 /pmc/articles/PMC10531580/ /pubmed/37762418 http://dx.doi.org/10.3390/ijms241814115 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhao, Sen Cao, Jiaxue Sun, Yanjin Zhou, Helin Zhu, Qi Dai, Dinghui Zhan, Siyuan Guo, Jiazhong Zhong, Tao Wang, Linjie Li, Li Zhang, Hongping METTL3 Promotes the Differentiation of Goat Skeletal Muscle Satellite Cells by Regulating MEF2C mRNA Stability in a m(6)A-Dependent Manner |
title | METTL3 Promotes the Differentiation of Goat Skeletal Muscle Satellite Cells by Regulating MEF2C mRNA Stability in a m(6)A-Dependent Manner |
title_full | METTL3 Promotes the Differentiation of Goat Skeletal Muscle Satellite Cells by Regulating MEF2C mRNA Stability in a m(6)A-Dependent Manner |
title_fullStr | METTL3 Promotes the Differentiation of Goat Skeletal Muscle Satellite Cells by Regulating MEF2C mRNA Stability in a m(6)A-Dependent Manner |
title_full_unstemmed | METTL3 Promotes the Differentiation of Goat Skeletal Muscle Satellite Cells by Regulating MEF2C mRNA Stability in a m(6)A-Dependent Manner |
title_short | METTL3 Promotes the Differentiation of Goat Skeletal Muscle Satellite Cells by Regulating MEF2C mRNA Stability in a m(6)A-Dependent Manner |
title_sort | mettl3 promotes the differentiation of goat skeletal muscle satellite cells by regulating mef2c mrna stability in a m(6)a-dependent manner |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531580/ https://www.ncbi.nlm.nih.gov/pubmed/37762418 http://dx.doi.org/10.3390/ijms241814115 |
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