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Developmental Instability and Gene Dysregulation in an Extracted Tetraploid from Hexaploid Wheat

The BBAA subgenomes of hexaploid common wheat can be ‘extracted’ to constitute a viable and self-reproducing novel tetraploid wheat, termed extracted tetraploid wheat (ETW). Prior studies have shown ETW manifesting phenotypic abnormalities and alteration in gene expression and epigenetic modificatio...

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Autores principales: Li, Yang, Sha, Yan, Wang, Han, Lv, Ruili, Zhang, Deshi, Li, Shuhang, Xu, Chunming, Liu, Bao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531679/
https://www.ncbi.nlm.nih.gov/pubmed/37762338
http://dx.doi.org/10.3390/ijms241814037
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author Li, Yang
Sha, Yan
Wang, Han
Lv, Ruili
Zhang, Deshi
Li, Shuhang
Xu, Chunming
Liu, Bao
author_facet Li, Yang
Sha, Yan
Wang, Han
Lv, Ruili
Zhang, Deshi
Li, Shuhang
Xu, Chunming
Liu, Bao
author_sort Li, Yang
collection PubMed
description The BBAA subgenomes of hexaploid common wheat can be ‘extracted’ to constitute a viable and self-reproducing novel tetraploid wheat, termed extracted tetraploid wheat (ETW). Prior studies have shown ETW manifesting phenotypic abnormalities and alteration in gene expression and epigenetic modifications. No population level investigation has been conducted, leaving the issue unclear regarding whether developmental stability, an essential property evolved in all natural organisms, might have been undermined in ETW. Here, we measured variations in five morphological traits and somatic chromosomal stability in populations of ETW and of its hexaploid donor, a resynthesized hexaploid and a natural tetraploid wheat. We observed phenotypic defects in ETW. Meanwhile, we documented much greater within-population variations in ETW than in the other wheat genotypes, most probably due to disrupted developmental stability in ETW. Also, somatic structural chromosome variations were detected only in ETW. Comparative transcriptome analyses indicated that the disrupted developmental stability of ETW is likely linked to massive dysregulation of genome-wide gene expression rather than to genetic mutations. Population network analysis of gene expression implicated intrinsic connectivity among the variable traits, while gene set enrichment analysis provided possible links between dysregulated gene expression and interlaced trait variation.
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spelling pubmed-105316792023-09-28 Developmental Instability and Gene Dysregulation in an Extracted Tetraploid from Hexaploid Wheat Li, Yang Sha, Yan Wang, Han Lv, Ruili Zhang, Deshi Li, Shuhang Xu, Chunming Liu, Bao Int J Mol Sci Article The BBAA subgenomes of hexaploid common wheat can be ‘extracted’ to constitute a viable and self-reproducing novel tetraploid wheat, termed extracted tetraploid wheat (ETW). Prior studies have shown ETW manifesting phenotypic abnormalities and alteration in gene expression and epigenetic modifications. No population level investigation has been conducted, leaving the issue unclear regarding whether developmental stability, an essential property evolved in all natural organisms, might have been undermined in ETW. Here, we measured variations in five morphological traits and somatic chromosomal stability in populations of ETW and of its hexaploid donor, a resynthesized hexaploid and a natural tetraploid wheat. We observed phenotypic defects in ETW. Meanwhile, we documented much greater within-population variations in ETW than in the other wheat genotypes, most probably due to disrupted developmental stability in ETW. Also, somatic structural chromosome variations were detected only in ETW. Comparative transcriptome analyses indicated that the disrupted developmental stability of ETW is likely linked to massive dysregulation of genome-wide gene expression rather than to genetic mutations. Population network analysis of gene expression implicated intrinsic connectivity among the variable traits, while gene set enrichment analysis provided possible links between dysregulated gene expression and interlaced trait variation. MDPI 2023-09-13 /pmc/articles/PMC10531679/ /pubmed/37762338 http://dx.doi.org/10.3390/ijms241814037 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Yang
Sha, Yan
Wang, Han
Lv, Ruili
Zhang, Deshi
Li, Shuhang
Xu, Chunming
Liu, Bao
Developmental Instability and Gene Dysregulation in an Extracted Tetraploid from Hexaploid Wheat
title Developmental Instability and Gene Dysregulation in an Extracted Tetraploid from Hexaploid Wheat
title_full Developmental Instability and Gene Dysregulation in an Extracted Tetraploid from Hexaploid Wheat
title_fullStr Developmental Instability and Gene Dysregulation in an Extracted Tetraploid from Hexaploid Wheat
title_full_unstemmed Developmental Instability and Gene Dysregulation in an Extracted Tetraploid from Hexaploid Wheat
title_short Developmental Instability and Gene Dysregulation in an Extracted Tetraploid from Hexaploid Wheat
title_sort developmental instability and gene dysregulation in an extracted tetraploid from hexaploid wheat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531679/
https://www.ncbi.nlm.nih.gov/pubmed/37762338
http://dx.doi.org/10.3390/ijms241814037
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