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Salivary Lipids of Patients with Non-Small Cell Lung Cancer Show Perturbation with Respect to Plasma

A comprehensive lipid profile was analyzed in patients with non-small cell lung cancer (NSCLC) using nanoflow ultrahigh-performance liquid chromatography–electrospray ionization–tandem mass spectrometry. This study investigated 297 and 202 lipids in saliva and plasma samples, respectively, comparing...

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Autores principales: Hwang, Bo Young, Seo, Jae Won, Muftuoglu, Can, Mert, Ufuk, Guldaval, Filiz, Asadi, Milad, Karakus, Haydar Soydaner, Goksel, Tuncay, Veral, Ali, Caner, Ayse, Moon, Myeong Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531690/
https://www.ncbi.nlm.nih.gov/pubmed/37762567
http://dx.doi.org/10.3390/ijms241814264
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author Hwang, Bo Young
Seo, Jae Won
Muftuoglu, Can
Mert, Ufuk
Guldaval, Filiz
Asadi, Milad
Karakus, Haydar Soydaner
Goksel, Tuncay
Veral, Ali
Caner, Ayse
Moon, Myeong Hee
author_facet Hwang, Bo Young
Seo, Jae Won
Muftuoglu, Can
Mert, Ufuk
Guldaval, Filiz
Asadi, Milad
Karakus, Haydar Soydaner
Goksel, Tuncay
Veral, Ali
Caner, Ayse
Moon, Myeong Hee
author_sort Hwang, Bo Young
collection PubMed
description A comprehensive lipid profile was analyzed in patients with non-small cell lung cancer (NSCLC) using nanoflow ultrahigh-performance liquid chromatography–electrospray ionization–tandem mass spectrometry. This study investigated 297 and 202 lipids in saliva and plasma samples, respectively, comparing NSCLC patients to healthy controls. Lipids with significant changes (>2-fold, p < 0.05) were further analyzed in each sample type. Both saliva and plasma exhibited similar lipid alteration patterns in NSCLC, but saliva showed more pronounced changes. Total triglycerides (TGs) increased (>2–3-fold) in plasma and saliva samples. Three specific TGs (50:2, 52:5, and 54:6) were significantly increased in NSCLC for both sample types. A common ceramide species (d18:1/24:0) and phosphatidylinositol 38:4 decreased in both plasma and saliva by approximately two-fold. Phosphatidylserine 36:1 was selectively detected in saliva and showed a subsequent decrease, making it a potential biomarker for predicting lung cancer. We identified 27 salivary and 10 plasma lipids as candidate markers for NSCLC through statistical evaluations. Moreover, this study highlights the potential of saliva in understanding changes in lipid metabolism associated with NSCLC.
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spelling pubmed-105316902023-09-28 Salivary Lipids of Patients with Non-Small Cell Lung Cancer Show Perturbation with Respect to Plasma Hwang, Bo Young Seo, Jae Won Muftuoglu, Can Mert, Ufuk Guldaval, Filiz Asadi, Milad Karakus, Haydar Soydaner Goksel, Tuncay Veral, Ali Caner, Ayse Moon, Myeong Hee Int J Mol Sci Article A comprehensive lipid profile was analyzed in patients with non-small cell lung cancer (NSCLC) using nanoflow ultrahigh-performance liquid chromatography–electrospray ionization–tandem mass spectrometry. This study investigated 297 and 202 lipids in saliva and plasma samples, respectively, comparing NSCLC patients to healthy controls. Lipids with significant changes (>2-fold, p < 0.05) were further analyzed in each sample type. Both saliva and plasma exhibited similar lipid alteration patterns in NSCLC, but saliva showed more pronounced changes. Total triglycerides (TGs) increased (>2–3-fold) in plasma and saliva samples. Three specific TGs (50:2, 52:5, and 54:6) were significantly increased in NSCLC for both sample types. A common ceramide species (d18:1/24:0) and phosphatidylinositol 38:4 decreased in both plasma and saliva by approximately two-fold. Phosphatidylserine 36:1 was selectively detected in saliva and showed a subsequent decrease, making it a potential biomarker for predicting lung cancer. We identified 27 salivary and 10 plasma lipids as candidate markers for NSCLC through statistical evaluations. Moreover, this study highlights the potential of saliva in understanding changes in lipid metabolism associated with NSCLC. MDPI 2023-09-19 /pmc/articles/PMC10531690/ /pubmed/37762567 http://dx.doi.org/10.3390/ijms241814264 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hwang, Bo Young
Seo, Jae Won
Muftuoglu, Can
Mert, Ufuk
Guldaval, Filiz
Asadi, Milad
Karakus, Haydar Soydaner
Goksel, Tuncay
Veral, Ali
Caner, Ayse
Moon, Myeong Hee
Salivary Lipids of Patients with Non-Small Cell Lung Cancer Show Perturbation with Respect to Plasma
title Salivary Lipids of Patients with Non-Small Cell Lung Cancer Show Perturbation with Respect to Plasma
title_full Salivary Lipids of Patients with Non-Small Cell Lung Cancer Show Perturbation with Respect to Plasma
title_fullStr Salivary Lipids of Patients with Non-Small Cell Lung Cancer Show Perturbation with Respect to Plasma
title_full_unstemmed Salivary Lipids of Patients with Non-Small Cell Lung Cancer Show Perturbation with Respect to Plasma
title_short Salivary Lipids of Patients with Non-Small Cell Lung Cancer Show Perturbation with Respect to Plasma
title_sort salivary lipids of patients with non-small cell lung cancer show perturbation with respect to plasma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531690/
https://www.ncbi.nlm.nih.gov/pubmed/37762567
http://dx.doi.org/10.3390/ijms241814264
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