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Antibody Content against Epstein–Barr Virus in Blood Extracellular Vesicles Correlates with Disease Activity and Brain Volume in Patients with Relapsing–Remitting Multiple Sclerosis
We aimed to analyze whether EVs carry antibodies against EBV antigens and the possibility that they could serve as diagnostic and disease activity blood biomarkers in RRMS. This was a prospective and observational study including patients with RRMS with active and inactive disease and healthy contro...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531815/ https://www.ncbi.nlm.nih.gov/pubmed/37762495 http://dx.doi.org/10.3390/ijms241814192 |
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author | Fernández-Fournier, Mireya López-Molina, MariPaz Torres Iglesias, Gabriel Botella, Lucía Chamorro, Beatriz Laso-García, Fernando Puertas, Inmaculada Tallón Barranco, Antonio Otero-Ortega, Laura Frank-García, Ana Díez-Tejedor, Exuperio |
author_facet | Fernández-Fournier, Mireya López-Molina, MariPaz Torres Iglesias, Gabriel Botella, Lucía Chamorro, Beatriz Laso-García, Fernando Puertas, Inmaculada Tallón Barranco, Antonio Otero-Ortega, Laura Frank-García, Ana Díez-Tejedor, Exuperio |
author_sort | Fernández-Fournier, Mireya |
collection | PubMed |
description | We aimed to analyze whether EVs carry antibodies against EBV antigens and the possibility that they could serve as diagnostic and disease activity blood biomarkers in RRMS. This was a prospective and observational study including patients with RRMS with active and inactive disease and healthy controls. Blood EVs were isolated by precipitation. Titers of antibodies against nuclear (anti-EBNA1) and capsid (anti-VCA) EBV antigens in EVs and in plasma, as well as content of myelin antibodies in EVs were determined by ELISA. An exploratory analysis of correlations with clinical and radiological data was performed. Patients with RRMS had higher titers of anti-VCA inside EVs and free in plasma than healthy controls. Patients with active disease showed higher levels of anti-EBNA1 in EVs, but not in plasma, than patients with inactive disease. EV anti-VCA levels correlated with disease duration and with decreased brain volume structures—total brain, white matter, gray matter, cerebellum, hippocampus, —but not with T2/FLAIR lesion volume or EDSS, SDMT, or 9HPT. In addition, EV anti-VCA correlated with EV anti-MBP. The anti-VCA and anti-EBNA1 content in EVs could represent diagnostic and disease activity blood biomarkers, respectively, in RRMS. |
format | Online Article Text |
id | pubmed-10531815 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105318152023-09-28 Antibody Content against Epstein–Barr Virus in Blood Extracellular Vesicles Correlates with Disease Activity and Brain Volume in Patients with Relapsing–Remitting Multiple Sclerosis Fernández-Fournier, Mireya López-Molina, MariPaz Torres Iglesias, Gabriel Botella, Lucía Chamorro, Beatriz Laso-García, Fernando Puertas, Inmaculada Tallón Barranco, Antonio Otero-Ortega, Laura Frank-García, Ana Díez-Tejedor, Exuperio Int J Mol Sci Article We aimed to analyze whether EVs carry antibodies against EBV antigens and the possibility that they could serve as diagnostic and disease activity blood biomarkers in RRMS. This was a prospective and observational study including patients with RRMS with active and inactive disease and healthy controls. Blood EVs were isolated by precipitation. Titers of antibodies against nuclear (anti-EBNA1) and capsid (anti-VCA) EBV antigens in EVs and in plasma, as well as content of myelin antibodies in EVs were determined by ELISA. An exploratory analysis of correlations with clinical and radiological data was performed. Patients with RRMS had higher titers of anti-VCA inside EVs and free in plasma than healthy controls. Patients with active disease showed higher levels of anti-EBNA1 in EVs, but not in plasma, than patients with inactive disease. EV anti-VCA levels correlated with disease duration and with decreased brain volume structures—total brain, white matter, gray matter, cerebellum, hippocampus, —but not with T2/FLAIR lesion volume or EDSS, SDMT, or 9HPT. In addition, EV anti-VCA correlated with EV anti-MBP. The anti-VCA and anti-EBNA1 content in EVs could represent diagnostic and disease activity blood biomarkers, respectively, in RRMS. MDPI 2023-09-16 /pmc/articles/PMC10531815/ /pubmed/37762495 http://dx.doi.org/10.3390/ijms241814192 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fernández-Fournier, Mireya López-Molina, MariPaz Torres Iglesias, Gabriel Botella, Lucía Chamorro, Beatriz Laso-García, Fernando Puertas, Inmaculada Tallón Barranco, Antonio Otero-Ortega, Laura Frank-García, Ana Díez-Tejedor, Exuperio Antibody Content against Epstein–Barr Virus in Blood Extracellular Vesicles Correlates with Disease Activity and Brain Volume in Patients with Relapsing–Remitting Multiple Sclerosis |
title | Antibody Content against Epstein–Barr Virus in Blood Extracellular Vesicles Correlates with Disease Activity and Brain Volume in Patients with Relapsing–Remitting Multiple Sclerosis |
title_full | Antibody Content against Epstein–Barr Virus in Blood Extracellular Vesicles Correlates with Disease Activity and Brain Volume in Patients with Relapsing–Remitting Multiple Sclerosis |
title_fullStr | Antibody Content against Epstein–Barr Virus in Blood Extracellular Vesicles Correlates with Disease Activity and Brain Volume in Patients with Relapsing–Remitting Multiple Sclerosis |
title_full_unstemmed | Antibody Content against Epstein–Barr Virus in Blood Extracellular Vesicles Correlates with Disease Activity and Brain Volume in Patients with Relapsing–Remitting Multiple Sclerosis |
title_short | Antibody Content against Epstein–Barr Virus in Blood Extracellular Vesicles Correlates with Disease Activity and Brain Volume in Patients with Relapsing–Remitting Multiple Sclerosis |
title_sort | antibody content against epstein–barr virus in blood extracellular vesicles correlates with disease activity and brain volume in patients with relapsing–remitting multiple sclerosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531815/ https://www.ncbi.nlm.nih.gov/pubmed/37762495 http://dx.doi.org/10.3390/ijms241814192 |
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