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Antibody Content against Epstein–Barr Virus in Blood Extracellular Vesicles Correlates with Disease Activity and Brain Volume in Patients with Relapsing–Remitting Multiple Sclerosis

We aimed to analyze whether EVs carry antibodies against EBV antigens and the possibility that they could serve as diagnostic and disease activity blood biomarkers in RRMS. This was a prospective and observational study including patients with RRMS with active and inactive disease and healthy contro...

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Autores principales: Fernández-Fournier, Mireya, López-Molina, MariPaz, Torres Iglesias, Gabriel, Botella, Lucía, Chamorro, Beatriz, Laso-García, Fernando, Puertas, Inmaculada, Tallón Barranco, Antonio, Otero-Ortega, Laura, Frank-García, Ana, Díez-Tejedor, Exuperio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531815/
https://www.ncbi.nlm.nih.gov/pubmed/37762495
http://dx.doi.org/10.3390/ijms241814192
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author Fernández-Fournier, Mireya
López-Molina, MariPaz
Torres Iglesias, Gabriel
Botella, Lucía
Chamorro, Beatriz
Laso-García, Fernando
Puertas, Inmaculada
Tallón Barranco, Antonio
Otero-Ortega, Laura
Frank-García, Ana
Díez-Tejedor, Exuperio
author_facet Fernández-Fournier, Mireya
López-Molina, MariPaz
Torres Iglesias, Gabriel
Botella, Lucía
Chamorro, Beatriz
Laso-García, Fernando
Puertas, Inmaculada
Tallón Barranco, Antonio
Otero-Ortega, Laura
Frank-García, Ana
Díez-Tejedor, Exuperio
author_sort Fernández-Fournier, Mireya
collection PubMed
description We aimed to analyze whether EVs carry antibodies against EBV antigens and the possibility that they could serve as diagnostic and disease activity blood biomarkers in RRMS. This was a prospective and observational study including patients with RRMS with active and inactive disease and healthy controls. Blood EVs were isolated by precipitation. Titers of antibodies against nuclear (anti-EBNA1) and capsid (anti-VCA) EBV antigens in EVs and in plasma, as well as content of myelin antibodies in EVs were determined by ELISA. An exploratory analysis of correlations with clinical and radiological data was performed. Patients with RRMS had higher titers of anti-VCA inside EVs and free in plasma than healthy controls. Patients with active disease showed higher levels of anti-EBNA1 in EVs, but not in plasma, than patients with inactive disease. EV anti-VCA levels correlated with disease duration and with decreased brain volume structures—total brain, white matter, gray matter, cerebellum, hippocampus, —but not with T2/FLAIR lesion volume or EDSS, SDMT, or 9HPT. In addition, EV anti-VCA correlated with EV anti-MBP. The anti-VCA and anti-EBNA1 content in EVs could represent diagnostic and disease activity blood biomarkers, respectively, in RRMS.
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spelling pubmed-105318152023-09-28 Antibody Content against Epstein–Barr Virus in Blood Extracellular Vesicles Correlates with Disease Activity and Brain Volume in Patients with Relapsing–Remitting Multiple Sclerosis Fernández-Fournier, Mireya López-Molina, MariPaz Torres Iglesias, Gabriel Botella, Lucía Chamorro, Beatriz Laso-García, Fernando Puertas, Inmaculada Tallón Barranco, Antonio Otero-Ortega, Laura Frank-García, Ana Díez-Tejedor, Exuperio Int J Mol Sci Article We aimed to analyze whether EVs carry antibodies against EBV antigens and the possibility that they could serve as diagnostic and disease activity blood biomarkers in RRMS. This was a prospective and observational study including patients with RRMS with active and inactive disease and healthy controls. Blood EVs were isolated by precipitation. Titers of antibodies against nuclear (anti-EBNA1) and capsid (anti-VCA) EBV antigens in EVs and in plasma, as well as content of myelin antibodies in EVs were determined by ELISA. An exploratory analysis of correlations with clinical and radiological data was performed. Patients with RRMS had higher titers of anti-VCA inside EVs and free in plasma than healthy controls. Patients with active disease showed higher levels of anti-EBNA1 in EVs, but not in plasma, than patients with inactive disease. EV anti-VCA levels correlated with disease duration and with decreased brain volume structures—total brain, white matter, gray matter, cerebellum, hippocampus, —but not with T2/FLAIR lesion volume or EDSS, SDMT, or 9HPT. In addition, EV anti-VCA correlated with EV anti-MBP. The anti-VCA and anti-EBNA1 content in EVs could represent diagnostic and disease activity blood biomarkers, respectively, in RRMS. MDPI 2023-09-16 /pmc/articles/PMC10531815/ /pubmed/37762495 http://dx.doi.org/10.3390/ijms241814192 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fernández-Fournier, Mireya
López-Molina, MariPaz
Torres Iglesias, Gabriel
Botella, Lucía
Chamorro, Beatriz
Laso-García, Fernando
Puertas, Inmaculada
Tallón Barranco, Antonio
Otero-Ortega, Laura
Frank-García, Ana
Díez-Tejedor, Exuperio
Antibody Content against Epstein–Barr Virus in Blood Extracellular Vesicles Correlates with Disease Activity and Brain Volume in Patients with Relapsing–Remitting Multiple Sclerosis
title Antibody Content against Epstein–Barr Virus in Blood Extracellular Vesicles Correlates with Disease Activity and Brain Volume in Patients with Relapsing–Remitting Multiple Sclerosis
title_full Antibody Content against Epstein–Barr Virus in Blood Extracellular Vesicles Correlates with Disease Activity and Brain Volume in Patients with Relapsing–Remitting Multiple Sclerosis
title_fullStr Antibody Content against Epstein–Barr Virus in Blood Extracellular Vesicles Correlates with Disease Activity and Brain Volume in Patients with Relapsing–Remitting Multiple Sclerosis
title_full_unstemmed Antibody Content against Epstein–Barr Virus in Blood Extracellular Vesicles Correlates with Disease Activity and Brain Volume in Patients with Relapsing–Remitting Multiple Sclerosis
title_short Antibody Content against Epstein–Barr Virus in Blood Extracellular Vesicles Correlates with Disease Activity and Brain Volume in Patients with Relapsing–Remitting Multiple Sclerosis
title_sort antibody content against epstein–barr virus in blood extracellular vesicles correlates with disease activity and brain volume in patients with relapsing–remitting multiple sclerosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531815/
https://www.ncbi.nlm.nih.gov/pubmed/37762495
http://dx.doi.org/10.3390/ijms241814192
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