Cargando…
Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in Macrobrachium nipponense: Insights into Reproduction, Growth, and Sex Differentiation
This study investigated the potential to use double-stranded RNA insulin-like androgenic gland hormone (dsIAG) to induce sex reversal in Macrobrachium nipponense and identified the molecular mechanisms underlying crustacean reproduction and sex differentiation. The study aimed to determine whether d...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531965/ https://www.ncbi.nlm.nih.gov/pubmed/37762609 http://dx.doi.org/10.3390/ijms241814306 |
_version_ | 1785111844032086016 |
---|---|
author | Cai, Pengfei Zhang, Wenyi Jiang, Sufei Xiong, Yiwei Yuan, Huwei Gao, Zijian Gao, Xuanbing Ma, Cheng Zhou, Yongkang Gong, Yongsheng Qiao, Hui Jin, Shubo Fu, Hongtuo |
author_facet | Cai, Pengfei Zhang, Wenyi Jiang, Sufei Xiong, Yiwei Yuan, Huwei Gao, Zijian Gao, Xuanbing Ma, Cheng Zhou, Yongkang Gong, Yongsheng Qiao, Hui Jin, Shubo Fu, Hongtuo |
author_sort | Cai, Pengfei |
collection | PubMed |
description | This study investigated the potential to use double-stranded RNA insulin-like androgenic gland hormone (dsIAG) to induce sex reversal in Macrobrachium nipponense and identified the molecular mechanisms underlying crustacean reproduction and sex differentiation. The study aimed to determine whether dsIAG could induce sex reversal in PL30-male M. nipponense during a critical period. The sex-related genes were selected by performing the gonadal transcriptome analysis of normal male (dsM), normal female (dsFM), neo-female sex-reversed individuals (dsRM), and unreversed males (dsNRM). After six injections, the experiment finally resulted in a 20% production of dsRM. Histologically, dsRM ovaries developed slower than dsFM, but dsNRM spermathecae developed normally. A total of 1718, 1069, and 255 differentially expressed genes were identified through transcriptome sequencing of the gonads in three comparison groups, revealing crucial genes related to reproduction and sex differentiation, such as GnRHR, VGR, SG, and LWS. Principal Component Analysis (PCA) also distinguished dsM and dsRM very well. In addition, this study predicted that the eyestalks and the “phototransduction-fly” photoperiodic pathways of M. nipponense could play an important role in sex reversal. The enrichment of related pathways and growth traits in dsNRM were combined to establish that IAG played a significant role in reproduction, growth regulation, and metabolism. Finally, complete sex reversal may depend on specific stimuli at critical periods. Overall, this study provides valuable findings for the IAG regulation of sex differentiation, reproduction, and growth of M. nipponense in establishing a monoculture. |
format | Online Article Text |
id | pubmed-10531965 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105319652023-09-28 Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in Macrobrachium nipponense: Insights into Reproduction, Growth, and Sex Differentiation Cai, Pengfei Zhang, Wenyi Jiang, Sufei Xiong, Yiwei Yuan, Huwei Gao, Zijian Gao, Xuanbing Ma, Cheng Zhou, Yongkang Gong, Yongsheng Qiao, Hui Jin, Shubo Fu, Hongtuo Int J Mol Sci Article This study investigated the potential to use double-stranded RNA insulin-like androgenic gland hormone (dsIAG) to induce sex reversal in Macrobrachium nipponense and identified the molecular mechanisms underlying crustacean reproduction and sex differentiation. The study aimed to determine whether dsIAG could induce sex reversal in PL30-male M. nipponense during a critical period. The sex-related genes were selected by performing the gonadal transcriptome analysis of normal male (dsM), normal female (dsFM), neo-female sex-reversed individuals (dsRM), and unreversed males (dsNRM). After six injections, the experiment finally resulted in a 20% production of dsRM. Histologically, dsRM ovaries developed slower than dsFM, but dsNRM spermathecae developed normally. A total of 1718, 1069, and 255 differentially expressed genes were identified through transcriptome sequencing of the gonads in three comparison groups, revealing crucial genes related to reproduction and sex differentiation, such as GnRHR, VGR, SG, and LWS. Principal Component Analysis (PCA) also distinguished dsM and dsRM very well. In addition, this study predicted that the eyestalks and the “phototransduction-fly” photoperiodic pathways of M. nipponense could play an important role in sex reversal. The enrichment of related pathways and growth traits in dsNRM were combined to establish that IAG played a significant role in reproduction, growth regulation, and metabolism. Finally, complete sex reversal may depend on specific stimuli at critical periods. Overall, this study provides valuable findings for the IAG regulation of sex differentiation, reproduction, and growth of M. nipponense in establishing a monoculture. MDPI 2023-09-19 /pmc/articles/PMC10531965/ /pubmed/37762609 http://dx.doi.org/10.3390/ijms241814306 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cai, Pengfei Zhang, Wenyi Jiang, Sufei Xiong, Yiwei Yuan, Huwei Gao, Zijian Gao, Xuanbing Ma, Cheng Zhou, Yongkang Gong, Yongsheng Qiao, Hui Jin, Shubo Fu, Hongtuo Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in Macrobrachium nipponense: Insights into Reproduction, Growth, and Sex Differentiation |
title | Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in Macrobrachium nipponense: Insights into Reproduction, Growth, and Sex Differentiation |
title_full | Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in Macrobrachium nipponense: Insights into Reproduction, Growth, and Sex Differentiation |
title_fullStr | Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in Macrobrachium nipponense: Insights into Reproduction, Growth, and Sex Differentiation |
title_full_unstemmed | Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in Macrobrachium nipponense: Insights into Reproduction, Growth, and Sex Differentiation |
title_short | Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in Macrobrachium nipponense: Insights into Reproduction, Growth, and Sex Differentiation |
title_sort | insulin-like androgenic gland hormone induced sex reversal and molecular pathways in macrobrachium nipponense: insights into reproduction, growth, and sex differentiation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531965/ https://www.ncbi.nlm.nih.gov/pubmed/37762609 http://dx.doi.org/10.3390/ijms241814306 |
work_keys_str_mv | AT caipengfei insulinlikeandrogenicglandhormoneinducedsexreversalandmolecularpathwaysinmacrobrachiumnipponenseinsightsintoreproductiongrowthandsexdifferentiation AT zhangwenyi insulinlikeandrogenicglandhormoneinducedsexreversalandmolecularpathwaysinmacrobrachiumnipponenseinsightsintoreproductiongrowthandsexdifferentiation AT jiangsufei insulinlikeandrogenicglandhormoneinducedsexreversalandmolecularpathwaysinmacrobrachiumnipponenseinsightsintoreproductiongrowthandsexdifferentiation AT xiongyiwei insulinlikeandrogenicglandhormoneinducedsexreversalandmolecularpathwaysinmacrobrachiumnipponenseinsightsintoreproductiongrowthandsexdifferentiation AT yuanhuwei insulinlikeandrogenicglandhormoneinducedsexreversalandmolecularpathwaysinmacrobrachiumnipponenseinsightsintoreproductiongrowthandsexdifferentiation AT gaozijian insulinlikeandrogenicglandhormoneinducedsexreversalandmolecularpathwaysinmacrobrachiumnipponenseinsightsintoreproductiongrowthandsexdifferentiation AT gaoxuanbing insulinlikeandrogenicglandhormoneinducedsexreversalandmolecularpathwaysinmacrobrachiumnipponenseinsightsintoreproductiongrowthandsexdifferentiation AT macheng insulinlikeandrogenicglandhormoneinducedsexreversalandmolecularpathwaysinmacrobrachiumnipponenseinsightsintoreproductiongrowthandsexdifferentiation AT zhouyongkang insulinlikeandrogenicglandhormoneinducedsexreversalandmolecularpathwaysinmacrobrachiumnipponenseinsightsintoreproductiongrowthandsexdifferentiation AT gongyongsheng insulinlikeandrogenicglandhormoneinducedsexreversalandmolecularpathwaysinmacrobrachiumnipponenseinsightsintoreproductiongrowthandsexdifferentiation AT qiaohui insulinlikeandrogenicglandhormoneinducedsexreversalandmolecularpathwaysinmacrobrachiumnipponenseinsightsintoreproductiongrowthandsexdifferentiation AT jinshubo insulinlikeandrogenicglandhormoneinducedsexreversalandmolecularpathwaysinmacrobrachiumnipponenseinsightsintoreproductiongrowthandsexdifferentiation AT fuhongtuo insulinlikeandrogenicglandhormoneinducedsexreversalandmolecularpathwaysinmacrobrachiumnipponenseinsightsintoreproductiongrowthandsexdifferentiation |