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Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in Macrobrachium nipponense: Insights into Reproduction, Growth, and Sex Differentiation

This study investigated the potential to use double-stranded RNA insulin-like androgenic gland hormone (dsIAG) to induce sex reversal in Macrobrachium nipponense and identified the molecular mechanisms underlying crustacean reproduction and sex differentiation. The study aimed to determine whether d...

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Autores principales: Cai, Pengfei, Zhang, Wenyi, Jiang, Sufei, Xiong, Yiwei, Yuan, Huwei, Gao, Zijian, Gao, Xuanbing, Ma, Cheng, Zhou, Yongkang, Gong, Yongsheng, Qiao, Hui, Jin, Shubo, Fu, Hongtuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531965/
https://www.ncbi.nlm.nih.gov/pubmed/37762609
http://dx.doi.org/10.3390/ijms241814306
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author Cai, Pengfei
Zhang, Wenyi
Jiang, Sufei
Xiong, Yiwei
Yuan, Huwei
Gao, Zijian
Gao, Xuanbing
Ma, Cheng
Zhou, Yongkang
Gong, Yongsheng
Qiao, Hui
Jin, Shubo
Fu, Hongtuo
author_facet Cai, Pengfei
Zhang, Wenyi
Jiang, Sufei
Xiong, Yiwei
Yuan, Huwei
Gao, Zijian
Gao, Xuanbing
Ma, Cheng
Zhou, Yongkang
Gong, Yongsheng
Qiao, Hui
Jin, Shubo
Fu, Hongtuo
author_sort Cai, Pengfei
collection PubMed
description This study investigated the potential to use double-stranded RNA insulin-like androgenic gland hormone (dsIAG) to induce sex reversal in Macrobrachium nipponense and identified the molecular mechanisms underlying crustacean reproduction and sex differentiation. The study aimed to determine whether dsIAG could induce sex reversal in PL30-male M. nipponense during a critical period. The sex-related genes were selected by performing the gonadal transcriptome analysis of normal male (dsM), normal female (dsFM), neo-female sex-reversed individuals (dsRM), and unreversed males (dsNRM). After six injections, the experiment finally resulted in a 20% production of dsRM. Histologically, dsRM ovaries developed slower than dsFM, but dsNRM spermathecae developed normally. A total of 1718, 1069, and 255 differentially expressed genes were identified through transcriptome sequencing of the gonads in three comparison groups, revealing crucial genes related to reproduction and sex differentiation, such as GnRHR, VGR, SG, and LWS. Principal Component Analysis (PCA) also distinguished dsM and dsRM very well. In addition, this study predicted that the eyestalks and the “phototransduction-fly” photoperiodic pathways of M. nipponense could play an important role in sex reversal. The enrichment of related pathways and growth traits in dsNRM were combined to establish that IAG played a significant role in reproduction, growth regulation, and metabolism. Finally, complete sex reversal may depend on specific stimuli at critical periods. Overall, this study provides valuable findings for the IAG regulation of sex differentiation, reproduction, and growth of M. nipponense in establishing a monoculture.
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spelling pubmed-105319652023-09-28 Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in Macrobrachium nipponense: Insights into Reproduction, Growth, and Sex Differentiation Cai, Pengfei Zhang, Wenyi Jiang, Sufei Xiong, Yiwei Yuan, Huwei Gao, Zijian Gao, Xuanbing Ma, Cheng Zhou, Yongkang Gong, Yongsheng Qiao, Hui Jin, Shubo Fu, Hongtuo Int J Mol Sci Article This study investigated the potential to use double-stranded RNA insulin-like androgenic gland hormone (dsIAG) to induce sex reversal in Macrobrachium nipponense and identified the molecular mechanisms underlying crustacean reproduction and sex differentiation. The study aimed to determine whether dsIAG could induce sex reversal in PL30-male M. nipponense during a critical period. The sex-related genes were selected by performing the gonadal transcriptome analysis of normal male (dsM), normal female (dsFM), neo-female sex-reversed individuals (dsRM), and unreversed males (dsNRM). After six injections, the experiment finally resulted in a 20% production of dsRM. Histologically, dsRM ovaries developed slower than dsFM, but dsNRM spermathecae developed normally. A total of 1718, 1069, and 255 differentially expressed genes were identified through transcriptome sequencing of the gonads in three comparison groups, revealing crucial genes related to reproduction and sex differentiation, such as GnRHR, VGR, SG, and LWS. Principal Component Analysis (PCA) also distinguished dsM and dsRM very well. In addition, this study predicted that the eyestalks and the “phototransduction-fly” photoperiodic pathways of M. nipponense could play an important role in sex reversal. The enrichment of related pathways and growth traits in dsNRM were combined to establish that IAG played a significant role in reproduction, growth regulation, and metabolism. Finally, complete sex reversal may depend on specific stimuli at critical periods. Overall, this study provides valuable findings for the IAG regulation of sex differentiation, reproduction, and growth of M. nipponense in establishing a monoculture. MDPI 2023-09-19 /pmc/articles/PMC10531965/ /pubmed/37762609 http://dx.doi.org/10.3390/ijms241814306 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cai, Pengfei
Zhang, Wenyi
Jiang, Sufei
Xiong, Yiwei
Yuan, Huwei
Gao, Zijian
Gao, Xuanbing
Ma, Cheng
Zhou, Yongkang
Gong, Yongsheng
Qiao, Hui
Jin, Shubo
Fu, Hongtuo
Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in Macrobrachium nipponense: Insights into Reproduction, Growth, and Sex Differentiation
title Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in Macrobrachium nipponense: Insights into Reproduction, Growth, and Sex Differentiation
title_full Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in Macrobrachium nipponense: Insights into Reproduction, Growth, and Sex Differentiation
title_fullStr Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in Macrobrachium nipponense: Insights into Reproduction, Growth, and Sex Differentiation
title_full_unstemmed Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in Macrobrachium nipponense: Insights into Reproduction, Growth, and Sex Differentiation
title_short Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in Macrobrachium nipponense: Insights into Reproduction, Growth, and Sex Differentiation
title_sort insulin-like androgenic gland hormone induced sex reversal and molecular pathways in macrobrachium nipponense: insights into reproduction, growth, and sex differentiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531965/
https://www.ncbi.nlm.nih.gov/pubmed/37762609
http://dx.doi.org/10.3390/ijms241814306
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