Analysis of Individual [Formula: see text] O(2max) Responses during a Cardiopulmonary Exercise Test and the Verification Phase in Physically Active Women

This study aimed to investigate the test–retest reliability, mean, and individual responses in the measurement of maximal oxygen consumption ([Formula: see text] O(2max)) during a cardiopulmonary exercise test (CPET) and the verification phase during cycle ergometry in women. Nine women (22 ± 2 yrs, 166.0 ± 4.5 cm, 58.6 ± 7.7 kg) completed a CPET, passively rested for 5 min, and then completed a verification phase at 90% of peak power output to determine the highest [Formula: see text] O(2) from the CPET ([Formula: see text] O(2CPET)) and verification phase ([Formula: see text] O(2verification)) on 2 separate days. Analyses included a two-way repeated measures ANOVA, intraclass correlation coefficients (ICC(2,1)), standard errors of the measurement (SEM), minimal differences (MD), and coefficients of variation (CoV). There was no test (test 1 versus test 2) × method (CPET vs. verification phase) interaction (p = 0.896) and no main effect for method (p = 0.459). However, test 1 (39.2 mL·kg(−1)·min(−1)) was significantly higher than test 2 (38.3 mL·kg(−1)·min(−1)) (p = 0.043). The [Formula: see text] O(2CPET) (ICC = 0.984; CoV = 1.98%; SEM = 0.77 mL·kg(−1)·min(−1); MD = 2.14 mL·kg(−1)·min(−1)) and [Formula: see text] O(2verification) (ICC = 0.964; CoV = 3.30%; SEM = 1.27 mL·kg(−1)·min(−1); MD = 3.52 mL·kg(−1)·min(−1)) demonstrated “excellent” reliability. Two subjects demonstrated a test 1 [Formula: see text] O(2CPET) that exceeded the test 2 [Formula: see text] O(2CPET), and one subject demonstrated a test 1 [Formula: see text] O(2verification) that exceeded the test 2 [Formula: see text] O(2verification) by more than the respective CPET and verification phase MD. One subject demonstrated a [Formula: see text] O(2CPET) that exceeded the [Formula: see text] O(2verification), and one subject demonstrated a [Formula: see text] O(2verification) that exceeded the [Formula: see text] O(2CPET) by more than the MD. These results demonstrate the importance of examining the individual responses in the measurement of the [Formula: see text] O(2max) and suggest that the MD may be a useful threshold to quantify real individual changes in [Formula: see text] O(2).