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A Lipid Nanoparticle-Based Method for the Generation of Liver-Specific Knockout Mice
Knockout mice are useful tools that can provide information about the normal function of genes, including their biochemical, developmental, and physiological roles. One problem associated with the generation of knockout mice is that the loss of some genes of interest produces a lethal phenotype. The...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10532048/ https://www.ncbi.nlm.nih.gov/pubmed/37762602 http://dx.doi.org/10.3390/ijms241814299 |
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author | Morita, Sumiyo Horii, Takuro Kimura, Mika Kobayashi, Ryosuke Tanaka, Hiroki Akita, Hidetaka Hatada, Izuho |
author_facet | Morita, Sumiyo Horii, Takuro Kimura, Mika Kobayashi, Ryosuke Tanaka, Hiroki Akita, Hidetaka Hatada, Izuho |
author_sort | Morita, Sumiyo |
collection | PubMed |
description | Knockout mice are useful tools that can provide information about the normal function of genes, including their biochemical, developmental, and physiological roles. One problem associated with the generation of knockout mice is that the loss of some genes of interest produces a lethal phenotype. Therefore, the use of conditioned knockout mice, in which genes are disrupted in specific organs, is essential for the elucidation of disease pathogenesis and the verification of drug targets. In general, conditional knockout mice are produced using the Cre/loxP system; however, the production of the large numbers of Cre/flox knockout and control mice required for analysis requires substantial time and effort. Here, we describe the generation of liver-specific conditional knockout mice via the introduction of lipid nanoparticles encapsulating Cre mRNA into the liver of floxed mice. This technique does not require the production of offspring by mating floxed mice and is therefore more convenient than the conventional method. The results presented here demonstrate that the LNP-based method enables liver-specific gene knockout in a short period of time. |
format | Online Article Text |
id | pubmed-10532048 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105320482023-09-28 A Lipid Nanoparticle-Based Method for the Generation of Liver-Specific Knockout Mice Morita, Sumiyo Horii, Takuro Kimura, Mika Kobayashi, Ryosuke Tanaka, Hiroki Akita, Hidetaka Hatada, Izuho Int J Mol Sci Communication Knockout mice are useful tools that can provide information about the normal function of genes, including their biochemical, developmental, and physiological roles. One problem associated with the generation of knockout mice is that the loss of some genes of interest produces a lethal phenotype. Therefore, the use of conditioned knockout mice, in which genes are disrupted in specific organs, is essential for the elucidation of disease pathogenesis and the verification of drug targets. In general, conditional knockout mice are produced using the Cre/loxP system; however, the production of the large numbers of Cre/flox knockout and control mice required for analysis requires substantial time and effort. Here, we describe the generation of liver-specific conditional knockout mice via the introduction of lipid nanoparticles encapsulating Cre mRNA into the liver of floxed mice. This technique does not require the production of offspring by mating floxed mice and is therefore more convenient than the conventional method. The results presented here demonstrate that the LNP-based method enables liver-specific gene knockout in a short period of time. MDPI 2023-09-19 /pmc/articles/PMC10532048/ /pubmed/37762602 http://dx.doi.org/10.3390/ijms241814299 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Morita, Sumiyo Horii, Takuro Kimura, Mika Kobayashi, Ryosuke Tanaka, Hiroki Akita, Hidetaka Hatada, Izuho A Lipid Nanoparticle-Based Method for the Generation of Liver-Specific Knockout Mice |
title | A Lipid Nanoparticle-Based Method for the Generation of Liver-Specific Knockout Mice |
title_full | A Lipid Nanoparticle-Based Method for the Generation of Liver-Specific Knockout Mice |
title_fullStr | A Lipid Nanoparticle-Based Method for the Generation of Liver-Specific Knockout Mice |
title_full_unstemmed | A Lipid Nanoparticle-Based Method for the Generation of Liver-Specific Knockout Mice |
title_short | A Lipid Nanoparticle-Based Method for the Generation of Liver-Specific Knockout Mice |
title_sort | lipid nanoparticle-based method for the generation of liver-specific knockout mice |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10532048/ https://www.ncbi.nlm.nih.gov/pubmed/37762602 http://dx.doi.org/10.3390/ijms241814299 |
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