Cargando…

Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores

Aspergillus series Versicolores are molds distributed among 17 species, commonly found in our environment, and responsible for infections. Since 2022, a new taxonomy has grouped them into 4 major lineages: A. versicolor, A. subversicolor, A. sydowii, and A. creber. Matrix-Assisted Laser Desorption/I...

Descripción completa

Detalles Bibliográficos
Autores principales: Jomat, Océane, Géry, Antoine, Leudet, Astrid, Capitaine, Agathe, Garon, David, Bonhomme, Julie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10532193/
https://www.ncbi.nlm.nih.gov/pubmed/37754976
http://dx.doi.org/10.3390/jof9090868
_version_ 1785111898346225664
author Jomat, Océane
Géry, Antoine
Leudet, Astrid
Capitaine, Agathe
Garon, David
Bonhomme, Julie
author_facet Jomat, Océane
Géry, Antoine
Leudet, Astrid
Capitaine, Agathe
Garon, David
Bonhomme, Julie
author_sort Jomat, Océane
collection PubMed
description Aspergillus series Versicolores are molds distributed among 17 species, commonly found in our environment, and responsible for infections. Since 2022, a new taxonomy has grouped them into 4 major lineages: A. versicolor, A. subversicolor, A. sydowii, and A. creber. Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) could be a faster and more cost-effective alternative to molecular techniques for identifying them by developing a local database. To evaluate this technique, 30 isolates from Aspergillus series Versicolores were used. A total of 59 main spectra profiles (MSPs) were created in the local database. This protocol enabled accurate identification of 100% of the extracted isolates, of which 97% (29/30) were correctly identified with a log score ≥ 2.00. Some MSPs recorded as Aspergillus versicolor in the supplier’s database could lead to false identifications as they did not match with the correct lineages. Although the local database is still limited in the number and diversity of species of Aspergillus series Versicolores, it is sufficiently effective for correct lineage identification according to the latest taxonomic revision, and better than the MALDI-TOF MS supplier’s database. This technology could improve the speed and accuracy of routine fungal identification for these species.
format Online
Article
Text
id pubmed-10532193
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-105321932023-09-28 Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores Jomat, Océane Géry, Antoine Leudet, Astrid Capitaine, Agathe Garon, David Bonhomme, Julie J Fungi (Basel) Article Aspergillus series Versicolores are molds distributed among 17 species, commonly found in our environment, and responsible for infections. Since 2022, a new taxonomy has grouped them into 4 major lineages: A. versicolor, A. subversicolor, A. sydowii, and A. creber. Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) could be a faster and more cost-effective alternative to molecular techniques for identifying them by developing a local database. To evaluate this technique, 30 isolates from Aspergillus series Versicolores were used. A total of 59 main spectra profiles (MSPs) were created in the local database. This protocol enabled accurate identification of 100% of the extracted isolates, of which 97% (29/30) were correctly identified with a log score ≥ 2.00. Some MSPs recorded as Aspergillus versicolor in the supplier’s database could lead to false identifications as they did not match with the correct lineages. Although the local database is still limited in the number and diversity of species of Aspergillus series Versicolores, it is sufficiently effective for correct lineage identification according to the latest taxonomic revision, and better than the MALDI-TOF MS supplier’s database. This technology could improve the speed and accuracy of routine fungal identification for these species. MDPI 2023-08-23 /pmc/articles/PMC10532193/ /pubmed/37754976 http://dx.doi.org/10.3390/jof9090868 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jomat, Océane
Géry, Antoine
Leudet, Astrid
Capitaine, Agathe
Garon, David
Bonhomme, Julie
Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores
title Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores
title_full Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores
title_fullStr Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores
title_full_unstemmed Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores
title_short Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores
title_sort spectrometric characterization of clinical and environmental isolates of aspergillus series versicolores
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10532193/
https://www.ncbi.nlm.nih.gov/pubmed/37754976
http://dx.doi.org/10.3390/jof9090868
work_keys_str_mv AT jomatoceane spectrometriccharacterizationofclinicalandenvironmentalisolatesofaspergillusseriesversicolores
AT geryantoine spectrometriccharacterizationofclinicalandenvironmentalisolatesofaspergillusseriesversicolores
AT leudetastrid spectrometriccharacterizationofclinicalandenvironmentalisolatesofaspergillusseriesversicolores
AT capitaineagathe spectrometriccharacterizationofclinicalandenvironmentalisolatesofaspergillusseriesversicolores
AT garondavid spectrometriccharacterizationofclinicalandenvironmentalisolatesofaspergillusseriesversicolores
AT bonhommejulie spectrometriccharacterizationofclinicalandenvironmentalisolatesofaspergillusseriesversicolores