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Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores
Aspergillus series Versicolores are molds distributed among 17 species, commonly found in our environment, and responsible for infections. Since 2022, a new taxonomy has grouped them into 4 major lineages: A. versicolor, A. subversicolor, A. sydowii, and A. creber. Matrix-Assisted Laser Desorption/I...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10532193/ https://www.ncbi.nlm.nih.gov/pubmed/37754976 http://dx.doi.org/10.3390/jof9090868 |
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author | Jomat, Océane Géry, Antoine Leudet, Astrid Capitaine, Agathe Garon, David Bonhomme, Julie |
author_facet | Jomat, Océane Géry, Antoine Leudet, Astrid Capitaine, Agathe Garon, David Bonhomme, Julie |
author_sort | Jomat, Océane |
collection | PubMed |
description | Aspergillus series Versicolores are molds distributed among 17 species, commonly found in our environment, and responsible for infections. Since 2022, a new taxonomy has grouped them into 4 major lineages: A. versicolor, A. subversicolor, A. sydowii, and A. creber. Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) could be a faster and more cost-effective alternative to molecular techniques for identifying them by developing a local database. To evaluate this technique, 30 isolates from Aspergillus series Versicolores were used. A total of 59 main spectra profiles (MSPs) were created in the local database. This protocol enabled accurate identification of 100% of the extracted isolates, of which 97% (29/30) were correctly identified with a log score ≥ 2.00. Some MSPs recorded as Aspergillus versicolor in the supplier’s database could lead to false identifications as they did not match with the correct lineages. Although the local database is still limited in the number and diversity of species of Aspergillus series Versicolores, it is sufficiently effective for correct lineage identification according to the latest taxonomic revision, and better than the MALDI-TOF MS supplier’s database. This technology could improve the speed and accuracy of routine fungal identification for these species. |
format | Online Article Text |
id | pubmed-10532193 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105321932023-09-28 Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores Jomat, Océane Géry, Antoine Leudet, Astrid Capitaine, Agathe Garon, David Bonhomme, Julie J Fungi (Basel) Article Aspergillus series Versicolores are molds distributed among 17 species, commonly found in our environment, and responsible for infections. Since 2022, a new taxonomy has grouped them into 4 major lineages: A. versicolor, A. subversicolor, A. sydowii, and A. creber. Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) could be a faster and more cost-effective alternative to molecular techniques for identifying them by developing a local database. To evaluate this technique, 30 isolates from Aspergillus series Versicolores were used. A total of 59 main spectra profiles (MSPs) were created in the local database. This protocol enabled accurate identification of 100% of the extracted isolates, of which 97% (29/30) were correctly identified with a log score ≥ 2.00. Some MSPs recorded as Aspergillus versicolor in the supplier’s database could lead to false identifications as they did not match with the correct lineages. Although the local database is still limited in the number and diversity of species of Aspergillus series Versicolores, it is sufficiently effective for correct lineage identification according to the latest taxonomic revision, and better than the MALDI-TOF MS supplier’s database. This technology could improve the speed and accuracy of routine fungal identification for these species. MDPI 2023-08-23 /pmc/articles/PMC10532193/ /pubmed/37754976 http://dx.doi.org/10.3390/jof9090868 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jomat, Océane Géry, Antoine Leudet, Astrid Capitaine, Agathe Garon, David Bonhomme, Julie Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores |
title | Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores |
title_full | Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores |
title_fullStr | Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores |
title_full_unstemmed | Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores |
title_short | Spectrometric Characterization of Clinical and Environmental Isolates of Aspergillus Series Versicolores |
title_sort | spectrometric characterization of clinical and environmental isolates of aspergillus series versicolores |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10532193/ https://www.ncbi.nlm.nih.gov/pubmed/37754976 http://dx.doi.org/10.3390/jof9090868 |
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