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Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis
Endometriosis is a chronic disease in which the endometrium cells are located outside the uterine cavity. The aim of this study was to evaluate circulating 20S proteasome and 20S immunoproteasome levels in plasma and peritoneal fluid in women with and without endometriosis in order to assess their u...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10532336/ https://www.ncbi.nlm.nih.gov/pubmed/37762666 http://dx.doi.org/10.3390/ijms241814363 |
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author | Wróbel, Monika Zuzanna, Zielińska Ołdak, Łukasz Kalicka, Aleksandra Mańka, Grzegorz Kiecka, Mariusz Spaczyński, Robert Z. Piekarski, Piotr Banaszewska, Beata Jakimiuk, Artur Issat, Tadeusz Rokita, Wojciech Młodawski, Jakub Szubert, Maria Sieroszewski, Piotr Raba, Grzegorz Szczupak, Kamil Kluz, Tomasz Kluza, Marek Pierzyński, Piotr Wojtyła, Cezary Lipa, Michał Warzecha, Damian Wielgoś, Mirosław Sawicki, Włodzimierz Gorodkiewicz, Ewa Laudański, Piotr |
author_facet | Wróbel, Monika Zuzanna, Zielińska Ołdak, Łukasz Kalicka, Aleksandra Mańka, Grzegorz Kiecka, Mariusz Spaczyński, Robert Z. Piekarski, Piotr Banaszewska, Beata Jakimiuk, Artur Issat, Tadeusz Rokita, Wojciech Młodawski, Jakub Szubert, Maria Sieroszewski, Piotr Raba, Grzegorz Szczupak, Kamil Kluz, Tomasz Kluza, Marek Pierzyński, Piotr Wojtyła, Cezary Lipa, Michał Warzecha, Damian Wielgoś, Mirosław Sawicki, Włodzimierz Gorodkiewicz, Ewa Laudański, Piotr |
author_sort | Wróbel, Monika |
collection | PubMed |
description | Endometriosis is a chronic disease in which the endometrium cells are located outside the uterine cavity. The aim of this study was to evaluate circulating 20S proteasome and 20S immunoproteasome levels in plasma and peritoneal fluid in women with and without endometriosis in order to assess their usefulness as biomarkers of disease. Concentrations were measured using surface plasmon resonance imaging biosensors. Patients with suspected endometriosis were included in the study—plasma was collected in 112 cases and peritoneal fluid in 75. Based on the presence of endometriosis lesions detected during laparoscopy, patients were divided into a study group (confirmed endometriosis) and a control group (patients without endometriosis). Proteasome and immunoproteasome levels in both the plasma (p = 0.174; p = 0.696, respectively) and the peritoneal fluid (p = 0.909; p = 0.284, respectively) did not differ between those groups. There was a statistically significant difference in the plasma proteasome levels between patients in the control group and those with mild (Stage I and II) endometriosis (p = 0.047) and in the plasma immunoproteasome levels in patients with ovarian cysts compared to those without (p = 0.017). The results of our study do not support the relevance of proteasome and immunoproteasome determination as biomarkers of the disease but suggest a potentially active role in the pathogenesis of endometriosis. |
format | Online Article Text |
id | pubmed-10532336 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105323362023-09-28 Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis Wróbel, Monika Zuzanna, Zielińska Ołdak, Łukasz Kalicka, Aleksandra Mańka, Grzegorz Kiecka, Mariusz Spaczyński, Robert Z. Piekarski, Piotr Banaszewska, Beata Jakimiuk, Artur Issat, Tadeusz Rokita, Wojciech Młodawski, Jakub Szubert, Maria Sieroszewski, Piotr Raba, Grzegorz Szczupak, Kamil Kluz, Tomasz Kluza, Marek Pierzyński, Piotr Wojtyła, Cezary Lipa, Michał Warzecha, Damian Wielgoś, Mirosław Sawicki, Włodzimierz Gorodkiewicz, Ewa Laudański, Piotr Int J Mol Sci Article Endometriosis is a chronic disease in which the endometrium cells are located outside the uterine cavity. The aim of this study was to evaluate circulating 20S proteasome and 20S immunoproteasome levels in plasma and peritoneal fluid in women with and without endometriosis in order to assess their usefulness as biomarkers of disease. Concentrations were measured using surface plasmon resonance imaging biosensors. Patients with suspected endometriosis were included in the study—plasma was collected in 112 cases and peritoneal fluid in 75. Based on the presence of endometriosis lesions detected during laparoscopy, patients were divided into a study group (confirmed endometriosis) and a control group (patients without endometriosis). Proteasome and immunoproteasome levels in both the plasma (p = 0.174; p = 0.696, respectively) and the peritoneal fluid (p = 0.909; p = 0.284, respectively) did not differ between those groups. There was a statistically significant difference in the plasma proteasome levels between patients in the control group and those with mild (Stage I and II) endometriosis (p = 0.047) and in the plasma immunoproteasome levels in patients with ovarian cysts compared to those without (p = 0.017). The results of our study do not support the relevance of proteasome and immunoproteasome determination as biomarkers of the disease but suggest a potentially active role in the pathogenesis of endometriosis. MDPI 2023-09-21 /pmc/articles/PMC10532336/ /pubmed/37762666 http://dx.doi.org/10.3390/ijms241814363 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wróbel, Monika Zuzanna, Zielińska Ołdak, Łukasz Kalicka, Aleksandra Mańka, Grzegorz Kiecka, Mariusz Spaczyński, Robert Z. Piekarski, Piotr Banaszewska, Beata Jakimiuk, Artur Issat, Tadeusz Rokita, Wojciech Młodawski, Jakub Szubert, Maria Sieroszewski, Piotr Raba, Grzegorz Szczupak, Kamil Kluz, Tomasz Kluza, Marek Pierzyński, Piotr Wojtyła, Cezary Lipa, Michał Warzecha, Damian Wielgoś, Mirosław Sawicki, Włodzimierz Gorodkiewicz, Ewa Laudański, Piotr Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis |
title | Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis |
title_full | Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis |
title_fullStr | Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis |
title_full_unstemmed | Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis |
title_short | Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis |
title_sort | evaluation of proteasome and immunoproteasome levels in plasma and peritoneal fluid in patients with endometriosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10532336/ https://www.ncbi.nlm.nih.gov/pubmed/37762666 http://dx.doi.org/10.3390/ijms241814363 |
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