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Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis

Endometriosis is a chronic disease in which the endometrium cells are located outside the uterine cavity. The aim of this study was to evaluate circulating 20S proteasome and 20S immunoproteasome levels in plasma and peritoneal fluid in women with and without endometriosis in order to assess their u...

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Autores principales: Wróbel, Monika, Zuzanna, Zielińska, Ołdak, Łukasz, Kalicka, Aleksandra, Mańka, Grzegorz, Kiecka, Mariusz, Spaczyński, Robert Z., Piekarski, Piotr, Banaszewska, Beata, Jakimiuk, Artur, Issat, Tadeusz, Rokita, Wojciech, Młodawski, Jakub, Szubert, Maria, Sieroszewski, Piotr, Raba, Grzegorz, Szczupak, Kamil, Kluz, Tomasz, Kluza, Marek, Pierzyński, Piotr, Wojtyła, Cezary, Lipa, Michał, Warzecha, Damian, Wielgoś, Mirosław, Sawicki, Włodzimierz, Gorodkiewicz, Ewa, Laudański, Piotr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10532336/
https://www.ncbi.nlm.nih.gov/pubmed/37762666
http://dx.doi.org/10.3390/ijms241814363
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author Wróbel, Monika
Zuzanna, Zielińska
Ołdak, Łukasz
Kalicka, Aleksandra
Mańka, Grzegorz
Kiecka, Mariusz
Spaczyński, Robert Z.
Piekarski, Piotr
Banaszewska, Beata
Jakimiuk, Artur
Issat, Tadeusz
Rokita, Wojciech
Młodawski, Jakub
Szubert, Maria
Sieroszewski, Piotr
Raba, Grzegorz
Szczupak, Kamil
Kluz, Tomasz
Kluza, Marek
Pierzyński, Piotr
Wojtyła, Cezary
Lipa, Michał
Warzecha, Damian
Wielgoś, Mirosław
Sawicki, Włodzimierz
Gorodkiewicz, Ewa
Laudański, Piotr
author_facet Wróbel, Monika
Zuzanna, Zielińska
Ołdak, Łukasz
Kalicka, Aleksandra
Mańka, Grzegorz
Kiecka, Mariusz
Spaczyński, Robert Z.
Piekarski, Piotr
Banaszewska, Beata
Jakimiuk, Artur
Issat, Tadeusz
Rokita, Wojciech
Młodawski, Jakub
Szubert, Maria
Sieroszewski, Piotr
Raba, Grzegorz
Szczupak, Kamil
Kluz, Tomasz
Kluza, Marek
Pierzyński, Piotr
Wojtyła, Cezary
Lipa, Michał
Warzecha, Damian
Wielgoś, Mirosław
Sawicki, Włodzimierz
Gorodkiewicz, Ewa
Laudański, Piotr
author_sort Wróbel, Monika
collection PubMed
description Endometriosis is a chronic disease in which the endometrium cells are located outside the uterine cavity. The aim of this study was to evaluate circulating 20S proteasome and 20S immunoproteasome levels in plasma and peritoneal fluid in women with and without endometriosis in order to assess their usefulness as biomarkers of disease. Concentrations were measured using surface plasmon resonance imaging biosensors. Patients with suspected endometriosis were included in the study—plasma was collected in 112 cases and peritoneal fluid in 75. Based on the presence of endometriosis lesions detected during laparoscopy, patients were divided into a study group (confirmed endometriosis) and a control group (patients without endometriosis). Proteasome and immunoproteasome levels in both the plasma (p = 0.174; p = 0.696, respectively) and the peritoneal fluid (p = 0.909; p = 0.284, respectively) did not differ between those groups. There was a statistically significant difference in the plasma proteasome levels between patients in the control group and those with mild (Stage I and II) endometriosis (p = 0.047) and in the plasma immunoproteasome levels in patients with ovarian cysts compared to those without (p = 0.017). The results of our study do not support the relevance of proteasome and immunoproteasome determination as biomarkers of the disease but suggest a potentially active role in the pathogenesis of endometriosis.
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spelling pubmed-105323362023-09-28 Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis Wróbel, Monika Zuzanna, Zielińska Ołdak, Łukasz Kalicka, Aleksandra Mańka, Grzegorz Kiecka, Mariusz Spaczyński, Robert Z. Piekarski, Piotr Banaszewska, Beata Jakimiuk, Artur Issat, Tadeusz Rokita, Wojciech Młodawski, Jakub Szubert, Maria Sieroszewski, Piotr Raba, Grzegorz Szczupak, Kamil Kluz, Tomasz Kluza, Marek Pierzyński, Piotr Wojtyła, Cezary Lipa, Michał Warzecha, Damian Wielgoś, Mirosław Sawicki, Włodzimierz Gorodkiewicz, Ewa Laudański, Piotr Int J Mol Sci Article Endometriosis is a chronic disease in which the endometrium cells are located outside the uterine cavity. The aim of this study was to evaluate circulating 20S proteasome and 20S immunoproteasome levels in plasma and peritoneal fluid in women with and without endometriosis in order to assess their usefulness as biomarkers of disease. Concentrations were measured using surface plasmon resonance imaging biosensors. Patients with suspected endometriosis were included in the study—plasma was collected in 112 cases and peritoneal fluid in 75. Based on the presence of endometriosis lesions detected during laparoscopy, patients were divided into a study group (confirmed endometriosis) and a control group (patients without endometriosis). Proteasome and immunoproteasome levels in both the plasma (p = 0.174; p = 0.696, respectively) and the peritoneal fluid (p = 0.909; p = 0.284, respectively) did not differ between those groups. There was a statistically significant difference in the plasma proteasome levels between patients in the control group and those with mild (Stage I and II) endometriosis (p = 0.047) and in the plasma immunoproteasome levels in patients with ovarian cysts compared to those without (p = 0.017). The results of our study do not support the relevance of proteasome and immunoproteasome determination as biomarkers of the disease but suggest a potentially active role in the pathogenesis of endometriosis. MDPI 2023-09-21 /pmc/articles/PMC10532336/ /pubmed/37762666 http://dx.doi.org/10.3390/ijms241814363 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wróbel, Monika
Zuzanna, Zielińska
Ołdak, Łukasz
Kalicka, Aleksandra
Mańka, Grzegorz
Kiecka, Mariusz
Spaczyński, Robert Z.
Piekarski, Piotr
Banaszewska, Beata
Jakimiuk, Artur
Issat, Tadeusz
Rokita, Wojciech
Młodawski, Jakub
Szubert, Maria
Sieroszewski, Piotr
Raba, Grzegorz
Szczupak, Kamil
Kluz, Tomasz
Kluza, Marek
Pierzyński, Piotr
Wojtyła, Cezary
Lipa, Michał
Warzecha, Damian
Wielgoś, Mirosław
Sawicki, Włodzimierz
Gorodkiewicz, Ewa
Laudański, Piotr
Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis
title Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis
title_full Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis
title_fullStr Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis
title_full_unstemmed Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis
title_short Evaluation of Proteasome and Immunoproteasome Levels in Plasma and Peritoneal Fluid in Patients with Endometriosis
title_sort evaluation of proteasome and immunoproteasome levels in plasma and peritoneal fluid in patients with endometriosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10532336/
https://www.ncbi.nlm.nih.gov/pubmed/37762666
http://dx.doi.org/10.3390/ijms241814363
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