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Polyphenol Analysis via LC-MS-ESI and Potent Antioxidant, Anti-Inflammatory, and Antimicrobial Activities of Jatropha multifida L. Extracts Used in Benin Pharmacopoeia
Jatropha multifida L., a plant from the Euphorbiaceae family, is commonly used in Benin’s traditional medicine due to its therapeutic benefits. This study aims to explore the medicinal efficacy of Jatropha multifida L. by evaluating its various biological activities. An initial phytochemical analysi...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10532662/ https://www.ncbi.nlm.nih.gov/pubmed/37763302 http://dx.doi.org/10.3390/life13091898 |
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author | Dah-Nouvlessounon, Durand Chokki, Michaelle Agossou, Essé A. Houédanou, Jean-Baptiste Nounagnon, Martial Sina, Haziz Vulturar, Romana Heghes, Simona Codruta Cozma, Angela Mavoungou, Jacques François Fodor, Adriana Baba-Moussa, Farid Suharoschi, Ramona Baba-Moussa, Lamine |
author_facet | Dah-Nouvlessounon, Durand Chokki, Michaelle Agossou, Essé A. Houédanou, Jean-Baptiste Nounagnon, Martial Sina, Haziz Vulturar, Romana Heghes, Simona Codruta Cozma, Angela Mavoungou, Jacques François Fodor, Adriana Baba-Moussa, Farid Suharoschi, Ramona Baba-Moussa, Lamine |
author_sort | Dah-Nouvlessounon, Durand |
collection | PubMed |
description | Jatropha multifida L., a plant from the Euphorbiaceae family, is commonly used in Benin’s traditional medicine due to its therapeutic benefits. This study aims to explore the medicinal efficacy of Jatropha multifida L. by evaluating its various biological activities. An initial phytochemical analysis was conducted, following which the polyphenols and flavonoids were quantified and identified using LC-MS-ESI. The antimicrobial efficacy of the extracts was tested using agar diffusion. Their antioxidant capacity was assessed using several methods: DPPH radical reduction, ABTS radical cation reduction, ferric ion (FRAP) reduction, and lipid peroxidation (LPO). Anti-inflammatory activity was determined based on the inhibition of protein (specifically albumin) denaturation. The study identified several phenolic and flavonoid compounds, including 2-Hydroxybenzoic acid, o-Coumaroylquinic acid, Apigenin-apiosyl-glucoside, and luteolin-galactoside. Notably, the extracts of J. multifida demonstrated bactericidal effects against a range of pathogens, with Concentration Minimally Bactericidal (CMB) values ranging from 22.67 mg/mL (for organisms such as S. aureus and C. albicans) to 47.61 mg/mL (for E. coli). Among the extracts, the ethanolic variant displayed the most potent DPPH radical scavenging activity, with an IC50 value of 0.72 ± 0.03 mg/mL. In contrast, the methanolic extract was superior in ferric ion reduction, registering 46.23 ± 1.10 µgEAA/g. Interestingly, the water-ethanolic extract surpassed others in the ABTS reduction method with a score of 0.49 ± 0.11 mol ET/g and also showcased the highest albumin denaturation inhibition rate of 97.31 ± 0.35% at a concentration of 1000 µg/mL. In conclusion, the extracts of Jatropha multifida L. are enriched with bioactive compounds that exhibit significant biological activities, underscoring their therapeutic potential. |
format | Online Article Text |
id | pubmed-10532662 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105326622023-09-28 Polyphenol Analysis via LC-MS-ESI and Potent Antioxidant, Anti-Inflammatory, and Antimicrobial Activities of Jatropha multifida L. Extracts Used in Benin Pharmacopoeia Dah-Nouvlessounon, Durand Chokki, Michaelle Agossou, Essé A. Houédanou, Jean-Baptiste Nounagnon, Martial Sina, Haziz Vulturar, Romana Heghes, Simona Codruta Cozma, Angela Mavoungou, Jacques François Fodor, Adriana Baba-Moussa, Farid Suharoschi, Ramona Baba-Moussa, Lamine Life (Basel) Article Jatropha multifida L., a plant from the Euphorbiaceae family, is commonly used in Benin’s traditional medicine due to its therapeutic benefits. This study aims to explore the medicinal efficacy of Jatropha multifida L. by evaluating its various biological activities. An initial phytochemical analysis was conducted, following which the polyphenols and flavonoids were quantified and identified using LC-MS-ESI. The antimicrobial efficacy of the extracts was tested using agar diffusion. Their antioxidant capacity was assessed using several methods: DPPH radical reduction, ABTS radical cation reduction, ferric ion (FRAP) reduction, and lipid peroxidation (LPO). Anti-inflammatory activity was determined based on the inhibition of protein (specifically albumin) denaturation. The study identified several phenolic and flavonoid compounds, including 2-Hydroxybenzoic acid, o-Coumaroylquinic acid, Apigenin-apiosyl-glucoside, and luteolin-galactoside. Notably, the extracts of J. multifida demonstrated bactericidal effects against a range of pathogens, with Concentration Minimally Bactericidal (CMB) values ranging from 22.67 mg/mL (for organisms such as S. aureus and C. albicans) to 47.61 mg/mL (for E. coli). Among the extracts, the ethanolic variant displayed the most potent DPPH radical scavenging activity, with an IC50 value of 0.72 ± 0.03 mg/mL. In contrast, the methanolic extract was superior in ferric ion reduction, registering 46.23 ± 1.10 µgEAA/g. Interestingly, the water-ethanolic extract surpassed others in the ABTS reduction method with a score of 0.49 ± 0.11 mol ET/g and also showcased the highest albumin denaturation inhibition rate of 97.31 ± 0.35% at a concentration of 1000 µg/mL. In conclusion, the extracts of Jatropha multifida L. are enriched with bioactive compounds that exhibit significant biological activities, underscoring their therapeutic potential. MDPI 2023-09-12 /pmc/articles/PMC10532662/ /pubmed/37763302 http://dx.doi.org/10.3390/life13091898 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dah-Nouvlessounon, Durand Chokki, Michaelle Agossou, Essé A. Houédanou, Jean-Baptiste Nounagnon, Martial Sina, Haziz Vulturar, Romana Heghes, Simona Codruta Cozma, Angela Mavoungou, Jacques François Fodor, Adriana Baba-Moussa, Farid Suharoschi, Ramona Baba-Moussa, Lamine Polyphenol Analysis via LC-MS-ESI and Potent Antioxidant, Anti-Inflammatory, and Antimicrobial Activities of Jatropha multifida L. Extracts Used in Benin Pharmacopoeia |
title | Polyphenol Analysis via LC-MS-ESI and Potent Antioxidant, Anti-Inflammatory, and Antimicrobial Activities of Jatropha multifida L. Extracts Used in Benin Pharmacopoeia |
title_full | Polyphenol Analysis via LC-MS-ESI and Potent Antioxidant, Anti-Inflammatory, and Antimicrobial Activities of Jatropha multifida L. Extracts Used in Benin Pharmacopoeia |
title_fullStr | Polyphenol Analysis via LC-MS-ESI and Potent Antioxidant, Anti-Inflammatory, and Antimicrobial Activities of Jatropha multifida L. Extracts Used in Benin Pharmacopoeia |
title_full_unstemmed | Polyphenol Analysis via LC-MS-ESI and Potent Antioxidant, Anti-Inflammatory, and Antimicrobial Activities of Jatropha multifida L. Extracts Used in Benin Pharmacopoeia |
title_short | Polyphenol Analysis via LC-MS-ESI and Potent Antioxidant, Anti-Inflammatory, and Antimicrobial Activities of Jatropha multifida L. Extracts Used in Benin Pharmacopoeia |
title_sort | polyphenol analysis via lc-ms-esi and potent antioxidant, anti-inflammatory, and antimicrobial activities of jatropha multifida l. extracts used in benin pharmacopoeia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10532662/ https://www.ncbi.nlm.nih.gov/pubmed/37763302 http://dx.doi.org/10.3390/life13091898 |
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