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Agonal Thrombus at Necropsy—A Third Category of Blood Coagulation in Domestic Carnivores

Agonal thrombus is infrequently discussed in veterinary forensic pathology, being misdiagnosed as postmortem coagulation. The main purpose of the present study is to confirm that agonal thrombosis is an important tool in ruling out sudden death and to characterize it by gross, histological, and immu...

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Autores principales: Tiu, Raluca Elena, Rizac, Raluca Ioana, Nicolae, George Laurentiu, Turbatu, Raluca Mihaela, Ciobotaru-Pirvu, Emilia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10532760/
https://www.ncbi.nlm.nih.gov/pubmed/37763238
http://dx.doi.org/10.3390/life13091834
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author Tiu, Raluca Elena
Rizac, Raluca Ioana
Nicolae, George Laurentiu
Turbatu, Raluca Mihaela
Ciobotaru-Pirvu, Emilia
author_facet Tiu, Raluca Elena
Rizac, Raluca Ioana
Nicolae, George Laurentiu
Turbatu, Raluca Mihaela
Ciobotaru-Pirvu, Emilia
author_sort Tiu, Raluca Elena
collection PubMed
description Agonal thrombus is infrequently discussed in veterinary forensic pathology, being misdiagnosed as postmortem coagulation. The main purpose of the present study is to confirm that agonal thrombosis is an important tool in ruling out sudden death and to characterize it by gross, histological, and immunohistochemical approaches. The investigations have been conducted on 56 domestic carnivores. Fibrin was observed as rosette—like arrangements around platelet aggregates, loose network, wave—like pattern or short fibers and the additional tendency of lines of Zahn being noted inconsistently. All agonal thrombi had positive reactions for anti—CD61 for platelets, disposed in variable—sized clumps or in a linear pattern close to endothelial cells of endocardium. The same positive reaction has been noted to anti—fibrinogen and anti—fibrin antibodies. CD45, CD68 and von Willebrand factor had a very low to absent expression. Cardiac lesions were found in 22 cases (39.29%) suggesting predisposition to agonal thrombosis in animals with cardiovascular diseases. The results prove that agonal thrombus is a third category of blood coagulation that forms strictly during agonal death. The microscopical findings describe the agonal thrombus similar to the morphology of recent thrombus vera. Given the results, the agonal thrombus is a useful tool that confirms the agonal suffering prior to death.
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spelling pubmed-105327602023-09-28 Agonal Thrombus at Necropsy—A Third Category of Blood Coagulation in Domestic Carnivores Tiu, Raluca Elena Rizac, Raluca Ioana Nicolae, George Laurentiu Turbatu, Raluca Mihaela Ciobotaru-Pirvu, Emilia Life (Basel) Article Agonal thrombus is infrequently discussed in veterinary forensic pathology, being misdiagnosed as postmortem coagulation. The main purpose of the present study is to confirm that agonal thrombosis is an important tool in ruling out sudden death and to characterize it by gross, histological, and immunohistochemical approaches. The investigations have been conducted on 56 domestic carnivores. Fibrin was observed as rosette—like arrangements around platelet aggregates, loose network, wave—like pattern or short fibers and the additional tendency of lines of Zahn being noted inconsistently. All agonal thrombi had positive reactions for anti—CD61 for platelets, disposed in variable—sized clumps or in a linear pattern close to endothelial cells of endocardium. The same positive reaction has been noted to anti—fibrinogen and anti—fibrin antibodies. CD45, CD68 and von Willebrand factor had a very low to absent expression. Cardiac lesions were found in 22 cases (39.29%) suggesting predisposition to agonal thrombosis in animals with cardiovascular diseases. The results prove that agonal thrombus is a third category of blood coagulation that forms strictly during agonal death. The microscopical findings describe the agonal thrombus similar to the morphology of recent thrombus vera. Given the results, the agonal thrombus is a useful tool that confirms the agonal suffering prior to death. MDPI 2023-08-30 /pmc/articles/PMC10532760/ /pubmed/37763238 http://dx.doi.org/10.3390/life13091834 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tiu, Raluca Elena
Rizac, Raluca Ioana
Nicolae, George Laurentiu
Turbatu, Raluca Mihaela
Ciobotaru-Pirvu, Emilia
Agonal Thrombus at Necropsy—A Third Category of Blood Coagulation in Domestic Carnivores
title Agonal Thrombus at Necropsy—A Third Category of Blood Coagulation in Domestic Carnivores
title_full Agonal Thrombus at Necropsy—A Third Category of Blood Coagulation in Domestic Carnivores
title_fullStr Agonal Thrombus at Necropsy—A Third Category of Blood Coagulation in Domestic Carnivores
title_full_unstemmed Agonal Thrombus at Necropsy—A Third Category of Blood Coagulation in Domestic Carnivores
title_short Agonal Thrombus at Necropsy—A Third Category of Blood Coagulation in Domestic Carnivores
title_sort agonal thrombus at necropsy—a third category of blood coagulation in domestic carnivores
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10532760/
https://www.ncbi.nlm.nih.gov/pubmed/37763238
http://dx.doi.org/10.3390/life13091834
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