Development of Simple Sequence Repeat Markers and Genetic Diversity Evaluation of Mycocentrospora acerina in Yunnan Province, China

Round spot is a destructive disease that limits of Panax notoginseng production in China. However, the genetic diversity of its etiological agent Mycocentrospora acerina has yet to be studied. In this work, firstly, we developed 32 M. acerina polymorphic microsatellite markers using MISA and CERVUS...

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Autores principales: Wang, Huiling, Yang, Kuan, Huang, Hongping, Guo, Liwei, He, Xiahong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10532959/
https://www.ncbi.nlm.nih.gov/pubmed/37755052
http://dx.doi.org/10.3390/jof9090944
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author Wang, Huiling
Yang, Kuan
Huang, Hongping
Guo, Liwei
He, Xiahong
author_facet Wang, Huiling
Yang, Kuan
Huang, Hongping
Guo, Liwei
He, Xiahong
author_sort Wang, Huiling
collection PubMed
description Round spot is a destructive disease that limits of Panax notoginseng production in China. However, the genetic diversity of its etiological agent Mycocentrospora acerina has yet to be studied. In this work, firstly, we developed 32 M. acerina polymorphic microsatellite markers using MISA and CERVUS 3.0 and selected 14 for further analysis. Then, we studied the genetic diversity of 187 isolates collected from P. notoginseng round spot using simple sequence repeat markers and polyacrylamide gel electrophoresis. The genetic diversity ranged from 0.813 to 0.946, with 264 alleles detected at the 14 microsatellite loci. The expected average heterozygosity was 0.897.
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spelling pubmed-105329592023-09-28 Development of Simple Sequence Repeat Markers and Genetic Diversity Evaluation of Mycocentrospora acerina in Yunnan Province, China Wang, Huiling Yang, Kuan Huang, Hongping Guo, Liwei He, Xiahong J Fungi (Basel) Article Round spot is a destructive disease that limits of Panax notoginseng production in China. However, the genetic diversity of its etiological agent Mycocentrospora acerina has yet to be studied. In this work, firstly, we developed 32 M. acerina polymorphic microsatellite markers using MISA and CERVUS 3.0 and selected 14 for further analysis. Then, we studied the genetic diversity of 187 isolates collected from P. notoginseng round spot using simple sequence repeat markers and polyacrylamide gel electrophoresis. The genetic diversity ranged from 0.813 to 0.946, with 264 alleles detected at the 14 microsatellite loci. The expected average heterozygosity was 0.897. MDPI 2023-09-19 /pmc/articles/PMC10532959/ /pubmed/37755052 http://dx.doi.org/10.3390/jof9090944 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Huiling
Yang, Kuan
Huang, Hongping
Guo, Liwei
He, Xiahong
Development of Simple Sequence Repeat Markers and Genetic Diversity Evaluation of Mycocentrospora acerina in Yunnan Province, China
title Development of Simple Sequence Repeat Markers and Genetic Diversity Evaluation of Mycocentrospora acerina in Yunnan Province, China
title_full Development of Simple Sequence Repeat Markers and Genetic Diversity Evaluation of Mycocentrospora acerina in Yunnan Province, China
title_fullStr Development of Simple Sequence Repeat Markers and Genetic Diversity Evaluation of Mycocentrospora acerina in Yunnan Province, China
title_full_unstemmed Development of Simple Sequence Repeat Markers and Genetic Diversity Evaluation of Mycocentrospora acerina in Yunnan Province, China
title_short Development of Simple Sequence Repeat Markers and Genetic Diversity Evaluation of Mycocentrospora acerina in Yunnan Province, China
title_sort development of simple sequence repeat markers and genetic diversity evaluation of mycocentrospora acerina in yunnan province, china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10532959/
https://www.ncbi.nlm.nih.gov/pubmed/37755052
http://dx.doi.org/10.3390/jof9090944
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