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Isorhamnetin Influences the Viability, Superoxide Production and Interleukin-8 Biosynthesis of Human Colorectal Adenocarcinoma HT-29 Cells In Vitro

Isorhamnetin has gained research interest for its anti-inflammatory, anti-proliferative and chemoprotective properties. In this study, human colon adenocarcinoma cells were cultured in the presence or absence of different isorhamnetin concentrations (5–150 μM) for 24 h or 48 h of cultivation to expl...

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Autores principales: Greifová, Hana, Tokárová, Katarína, Jambor, Tomáš, Štefunková, Nikola, Speváková, Ivana, Dupák, Rudolf, Balytska, Olha, Bažány, Denis, Capcarová, Marcela, Lukáč, Norbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10533024/
https://www.ncbi.nlm.nih.gov/pubmed/37763324
http://dx.doi.org/10.3390/life13091921
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author Greifová, Hana
Tokárová, Katarína
Jambor, Tomáš
Štefunková, Nikola
Speváková, Ivana
Dupák, Rudolf
Balytska, Olha
Bažány, Denis
Capcarová, Marcela
Lukáč, Norbert
author_facet Greifová, Hana
Tokárová, Katarína
Jambor, Tomáš
Štefunková, Nikola
Speváková, Ivana
Dupák, Rudolf
Balytska, Olha
Bažány, Denis
Capcarová, Marcela
Lukáč, Norbert
author_sort Greifová, Hana
collection PubMed
description Isorhamnetin has gained research interest for its anti-inflammatory, anti-proliferative and chemoprotective properties. In this study, human colon adenocarcinoma cells were cultured in the presence or absence of different isorhamnetin concentrations (5–150 μM) for 24 h or 48 h of cultivation to explore the impact on several parameters of viability/proliferation (mitochondrial function using an MTT test, metabolic activity, cell membrane integrity and lysosomal activity using a triple test). The intracellular generation of superoxide radicals using an NBT test and ELISA analysis was performed to observe the biosynthesis of interleukin 8 (IL-8) in cells stimulated with zymosan, as well as in basal conditions. The antiproliferative activity of isorhamnetin was demonstrated by significantly reduced values of mitochondrial and metabolic activity, integrity of cell membranes and lysosomal activity. Its high prooxidant potential was reflected by the significantly elevated generation of superoxides even in cells with low viability status. The anti-inflammatory effect of isorhamnetin was evident due to decreased IL-8 production, and the most significant decline in IL-8 concentration was observed after 24 h treatment in cells with induced inflammation. We demonstrated that isorhamnetin can suppress the proliferation of HT-29 cells, and this effect was correlated with pro-oxidative and anti-inflammatory activity of isorhamnetin.
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spelling pubmed-105330242023-09-28 Isorhamnetin Influences the Viability, Superoxide Production and Interleukin-8 Biosynthesis of Human Colorectal Adenocarcinoma HT-29 Cells In Vitro Greifová, Hana Tokárová, Katarína Jambor, Tomáš Štefunková, Nikola Speváková, Ivana Dupák, Rudolf Balytska, Olha Bažány, Denis Capcarová, Marcela Lukáč, Norbert Life (Basel) Article Isorhamnetin has gained research interest for its anti-inflammatory, anti-proliferative and chemoprotective properties. In this study, human colon adenocarcinoma cells were cultured in the presence or absence of different isorhamnetin concentrations (5–150 μM) for 24 h or 48 h of cultivation to explore the impact on several parameters of viability/proliferation (mitochondrial function using an MTT test, metabolic activity, cell membrane integrity and lysosomal activity using a triple test). The intracellular generation of superoxide radicals using an NBT test and ELISA analysis was performed to observe the biosynthesis of interleukin 8 (IL-8) in cells stimulated with zymosan, as well as in basal conditions. The antiproliferative activity of isorhamnetin was demonstrated by significantly reduced values of mitochondrial and metabolic activity, integrity of cell membranes and lysosomal activity. Its high prooxidant potential was reflected by the significantly elevated generation of superoxides even in cells with low viability status. The anti-inflammatory effect of isorhamnetin was evident due to decreased IL-8 production, and the most significant decline in IL-8 concentration was observed after 24 h treatment in cells with induced inflammation. We demonstrated that isorhamnetin can suppress the proliferation of HT-29 cells, and this effect was correlated with pro-oxidative and anti-inflammatory activity of isorhamnetin. MDPI 2023-09-15 /pmc/articles/PMC10533024/ /pubmed/37763324 http://dx.doi.org/10.3390/life13091921 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Greifová, Hana
Tokárová, Katarína
Jambor, Tomáš
Štefunková, Nikola
Speváková, Ivana
Dupák, Rudolf
Balytska, Olha
Bažány, Denis
Capcarová, Marcela
Lukáč, Norbert
Isorhamnetin Influences the Viability, Superoxide Production and Interleukin-8 Biosynthesis of Human Colorectal Adenocarcinoma HT-29 Cells In Vitro
title Isorhamnetin Influences the Viability, Superoxide Production and Interleukin-8 Biosynthesis of Human Colorectal Adenocarcinoma HT-29 Cells In Vitro
title_full Isorhamnetin Influences the Viability, Superoxide Production and Interleukin-8 Biosynthesis of Human Colorectal Adenocarcinoma HT-29 Cells In Vitro
title_fullStr Isorhamnetin Influences the Viability, Superoxide Production and Interleukin-8 Biosynthesis of Human Colorectal Adenocarcinoma HT-29 Cells In Vitro
title_full_unstemmed Isorhamnetin Influences the Viability, Superoxide Production and Interleukin-8 Biosynthesis of Human Colorectal Adenocarcinoma HT-29 Cells In Vitro
title_short Isorhamnetin Influences the Viability, Superoxide Production and Interleukin-8 Biosynthesis of Human Colorectal Adenocarcinoma HT-29 Cells In Vitro
title_sort isorhamnetin influences the viability, superoxide production and interleukin-8 biosynthesis of human colorectal adenocarcinoma ht-29 cells in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10533024/
https://www.ncbi.nlm.nih.gov/pubmed/37763324
http://dx.doi.org/10.3390/life13091921
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