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Demodex Species and Culturable Microorganism Co-Infestations in Patients with Blepharitis

We aimed to determine the prevalence of Demodex spp. and bacterial infection in patients with blepharitis and also to investigate the relationship between culturable microorganisms and Demodex spp. in this study. The study included patients diagnosed with blepharitis (n = 128) and volunteers without...

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Autores principales: Pyzia, Joanna, Mańkowska, Katarzyna, Czepita, Maciej, Kot, Karolina, Łanocha-Arendarczyk, Natalia, Czepita, Damian, Kosik-Bogacka, Danuta I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10533081/
https://www.ncbi.nlm.nih.gov/pubmed/37763231
http://dx.doi.org/10.3390/life13091827
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author Pyzia, Joanna
Mańkowska, Katarzyna
Czepita, Maciej
Kot, Karolina
Łanocha-Arendarczyk, Natalia
Czepita, Damian
Kosik-Bogacka, Danuta I.
author_facet Pyzia, Joanna
Mańkowska, Katarzyna
Czepita, Maciej
Kot, Karolina
Łanocha-Arendarczyk, Natalia
Czepita, Damian
Kosik-Bogacka, Danuta I.
author_sort Pyzia, Joanna
collection PubMed
description We aimed to determine the prevalence of Demodex spp. and bacterial infection in patients with blepharitis and also to investigate the relationship between culturable microorganisms and Demodex spp. in this study. The study included patients diagnosed with blepharitis (n = 128) and volunteers without ocular problems (n = 113). Eyelash sampling was performed by epilating eight lashes, which were then tested for Demodex spp. using a light microscope. The examination consisted of assessing the patient’s vision with and without ocular correction and tonus in both eyes and a careful examination of the anterior segment of both eyes. Bacterial identification was performed based on morphological, physiological, and biochemical methods. The prevalence of Demodex spp. was 8.0% in patients from the control group and all patients with blepharitis. Isolated forms of Demodex spp. were detected in all infested patients in the control group and in 58% of patients with blepharitis. A total of 35% of patients with blepharitis had from three to nine forms of Demodex spp., and 7% of patients with blepharitis had more than 10 mites in every field of vision. We found a statistically significant relationship between Demodex spp. infestation and the occurrence of eye dryness and sensations of burning and tearing, redness of the conjunctiva, feeling of a foreign body, loss of eyelashes, Meibomian gland dysfunction, and cylindrical dandruff. There were statistically significant relationships between Demodex sp. infestation and the presence of hyperopia, Meibomian cysts, chronic eyelid inflammation, and the use of eyeglasses. There was also a statistically significant relationship between the occurrence of Demodex spp. and seborrheic dermatitis and diabetes mellitus. Culturable microorganisms of the ocular surface were found in 8.7% of participants who were uninfested and in all patients infested with D. folliculorum. We isolated Staphylococcus aureus, Acinetobacter baumannii, Streptococcus pneumoniae, Klebsiella oxytoca, and Bacillus spp. from the conjunctival sac only in patients infested with D. folliculorum. This indicates an increased probability of colonization by pathogenic bacteria in patients with demodicosis. Therefore, patients infested with D. folliculorum should undergo a microbiological examination of conjunctival swabs.
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spelling pubmed-105330812023-09-28 Demodex Species and Culturable Microorganism Co-Infestations in Patients with Blepharitis Pyzia, Joanna Mańkowska, Katarzyna Czepita, Maciej Kot, Karolina Łanocha-Arendarczyk, Natalia Czepita, Damian Kosik-Bogacka, Danuta I. Life (Basel) Article We aimed to determine the prevalence of Demodex spp. and bacterial infection in patients with blepharitis and also to investigate the relationship between culturable microorganisms and Demodex spp. in this study. The study included patients diagnosed with blepharitis (n = 128) and volunteers without ocular problems (n = 113). Eyelash sampling was performed by epilating eight lashes, which were then tested for Demodex spp. using a light microscope. The examination consisted of assessing the patient’s vision with and without ocular correction and tonus in both eyes and a careful examination of the anterior segment of both eyes. Bacterial identification was performed based on morphological, physiological, and biochemical methods. The prevalence of Demodex spp. was 8.0% in patients from the control group and all patients with blepharitis. Isolated forms of Demodex spp. were detected in all infested patients in the control group and in 58% of patients with blepharitis. A total of 35% of patients with blepharitis had from three to nine forms of Demodex spp., and 7% of patients with blepharitis had more than 10 mites in every field of vision. We found a statistically significant relationship between Demodex spp. infestation and the occurrence of eye dryness and sensations of burning and tearing, redness of the conjunctiva, feeling of a foreign body, loss of eyelashes, Meibomian gland dysfunction, and cylindrical dandruff. There were statistically significant relationships between Demodex sp. infestation and the presence of hyperopia, Meibomian cysts, chronic eyelid inflammation, and the use of eyeglasses. There was also a statistically significant relationship between the occurrence of Demodex spp. and seborrheic dermatitis and diabetes mellitus. Culturable microorganisms of the ocular surface were found in 8.7% of participants who were uninfested and in all patients infested with D. folliculorum. We isolated Staphylococcus aureus, Acinetobacter baumannii, Streptococcus pneumoniae, Klebsiella oxytoca, and Bacillus spp. from the conjunctival sac only in patients infested with D. folliculorum. This indicates an increased probability of colonization by pathogenic bacteria in patients with demodicosis. Therefore, patients infested with D. folliculorum should undergo a microbiological examination of conjunctival swabs. MDPI 2023-08-29 /pmc/articles/PMC10533081/ /pubmed/37763231 http://dx.doi.org/10.3390/life13091827 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pyzia, Joanna
Mańkowska, Katarzyna
Czepita, Maciej
Kot, Karolina
Łanocha-Arendarczyk, Natalia
Czepita, Damian
Kosik-Bogacka, Danuta I.
Demodex Species and Culturable Microorganism Co-Infestations in Patients with Blepharitis
title Demodex Species and Culturable Microorganism Co-Infestations in Patients with Blepharitis
title_full Demodex Species and Culturable Microorganism Co-Infestations in Patients with Blepharitis
title_fullStr Demodex Species and Culturable Microorganism Co-Infestations in Patients with Blepharitis
title_full_unstemmed Demodex Species and Culturable Microorganism Co-Infestations in Patients with Blepharitis
title_short Demodex Species and Culturable Microorganism Co-Infestations in Patients with Blepharitis
title_sort demodex species and culturable microorganism co-infestations in patients with blepharitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10533081/
https://www.ncbi.nlm.nih.gov/pubmed/37763231
http://dx.doi.org/10.3390/life13091827
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