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Physicochemical properties of the vacuolar membrane and cellular factors determine formation of vacuolar invaginations
Vacuoles change their morphology in response to stress. In yeast exposed to chronically high temperatures, vacuolar membranes get deformed and invaginations are formed. We show that phase-separation of vacuolar membrane occurred after heat stress leading to the formation of the invagination. In addi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10533490/ https://www.ncbi.nlm.nih.gov/pubmed/37759072 http://dx.doi.org/10.1038/s41598-023-43232-5 |
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author | Kimura, Yoko Tsuji, Takuma Shimizu, Yosuke Watanabe, Yuki Kimura, Masafumi Fujimoto, Toyoshi Higuchi, Miyuki |
author_facet | Kimura, Yoko Tsuji, Takuma Shimizu, Yosuke Watanabe, Yuki Kimura, Masafumi Fujimoto, Toyoshi Higuchi, Miyuki |
author_sort | Kimura, Yoko |
collection | PubMed |
description | Vacuoles change their morphology in response to stress. In yeast exposed to chronically high temperatures, vacuolar membranes get deformed and invaginations are formed. We show that phase-separation of vacuolar membrane occurred after heat stress leading to the formation of the invagination. In addition, Hfl1, a vacuolar membrane-localized Atg8-binding protein, was found to suppress the excess vacuolar invaginations after heat stress. At that time, Hfl1 formed foci at the neck of the invaginations in wild-type cells, whereas it was efficiently degraded in the vacuole in the atg8Δ mutant. Genetic analysis showed that the endosomal sorting complex required for transport machinery was necessary to form the invaginations irrespective of Atg8 or Hfl1. In contrast, a combined mutation with the vacuole BAR domain protein Ivy1 led to vacuoles in hfl1Δivy1Δ and atg8Δivy1Δ mutants having constitutively invaginated structures; moreover, these mutants showed stress-sensitive phenotypes. Our findings suggest that vacuolar invaginations result from the combination of changes in the physiochemical properties of the vacuolar membrane and other cellular factors. |
format | Online Article Text |
id | pubmed-10533490 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-105334902023-09-29 Physicochemical properties of the vacuolar membrane and cellular factors determine formation of vacuolar invaginations Kimura, Yoko Tsuji, Takuma Shimizu, Yosuke Watanabe, Yuki Kimura, Masafumi Fujimoto, Toyoshi Higuchi, Miyuki Sci Rep Article Vacuoles change their morphology in response to stress. In yeast exposed to chronically high temperatures, vacuolar membranes get deformed and invaginations are formed. We show that phase-separation of vacuolar membrane occurred after heat stress leading to the formation of the invagination. In addition, Hfl1, a vacuolar membrane-localized Atg8-binding protein, was found to suppress the excess vacuolar invaginations after heat stress. At that time, Hfl1 formed foci at the neck of the invaginations in wild-type cells, whereas it was efficiently degraded in the vacuole in the atg8Δ mutant. Genetic analysis showed that the endosomal sorting complex required for transport machinery was necessary to form the invaginations irrespective of Atg8 or Hfl1. In contrast, a combined mutation with the vacuole BAR domain protein Ivy1 led to vacuoles in hfl1Δivy1Δ and atg8Δivy1Δ mutants having constitutively invaginated structures; moreover, these mutants showed stress-sensitive phenotypes. Our findings suggest that vacuolar invaginations result from the combination of changes in the physiochemical properties of the vacuolar membrane and other cellular factors. Nature Publishing Group UK 2023-09-27 /pmc/articles/PMC10533490/ /pubmed/37759072 http://dx.doi.org/10.1038/s41598-023-43232-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Kimura, Yoko Tsuji, Takuma Shimizu, Yosuke Watanabe, Yuki Kimura, Masafumi Fujimoto, Toyoshi Higuchi, Miyuki Physicochemical properties of the vacuolar membrane and cellular factors determine formation of vacuolar invaginations |
title | Physicochemical properties of the vacuolar membrane and cellular factors determine formation of vacuolar invaginations |
title_full | Physicochemical properties of the vacuolar membrane and cellular factors determine formation of vacuolar invaginations |
title_fullStr | Physicochemical properties of the vacuolar membrane and cellular factors determine formation of vacuolar invaginations |
title_full_unstemmed | Physicochemical properties of the vacuolar membrane and cellular factors determine formation of vacuolar invaginations |
title_short | Physicochemical properties of the vacuolar membrane and cellular factors determine formation of vacuolar invaginations |
title_sort | physicochemical properties of the vacuolar membrane and cellular factors determine formation of vacuolar invaginations |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10533490/ https://www.ncbi.nlm.nih.gov/pubmed/37759072 http://dx.doi.org/10.1038/s41598-023-43232-5 |
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