Cargando…

Advances in phage display based nano immunosensors for cholera toxin

Cholera, a persistent global public health concern, continues to cause outbreaks in approximately 30 countries and territories this year. The imperative to safeguard water sources and food from Vibrio cholerae, the causative pathogen, remains urgent. The bacterium is mainly disseminated via ingestio...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Yang, Yang, Kai-di, Kong, De-cai, Ye, Jun-feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10534012/
https://www.ncbi.nlm.nih.gov/pubmed/37781379
http://dx.doi.org/10.3389/fimmu.2023.1224397
_version_ 1785112301766967296
author Li, Yang
Yang, Kai-di
Kong, De-cai
Ye, Jun-feng
author_facet Li, Yang
Yang, Kai-di
Kong, De-cai
Ye, Jun-feng
author_sort Li, Yang
collection PubMed
description Cholera, a persistent global public health concern, continues to cause outbreaks in approximately 30 countries and territories this year. The imperative to safeguard water sources and food from Vibrio cholerae, the causative pathogen, remains urgent. The bacterium is mainly disseminated via ingestion of contaminated water or food. Despite the plate method’s gold standard status for detection, its time-consuming nature, taking several days to provide results, remains a challenge. The emergence of novel virulence serotypes raises public health concerns, potentially compromising existing detection methods. Hence, exploiting Vibrio cholerae toxin testing holds promise due to its inherent stability. Immunobiosensors, leveraging antibody specificity and sensitivity, present formidable tools for detecting diverse small molecules, encompassing drugs, hormones, toxins, and environmental pollutants. This review explores cholera toxin detection, highlighting phage display-based nano immunosensors’ potential. Engineered bacteriophages exhibit exceptional cholera toxin affinity, through specific antibody fragments or mimotopes, enabling precise quantification. This innovative approach promises to reshape cholera toxin detection, offering an alternative to animal-derived methods. Harnessing engineered bacteriophages aligns with ethical detection and emphasizes sensitivity and accuracy, a pivotal stride in the evolution of detection strategies. This review primarily introduces recent advancements in phage display-based nano immunosensors for cholera toxin, encompassing technical aspects, current challenges, and future prospects.
format Online
Article
Text
id pubmed-10534012
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-105340122023-09-29 Advances in phage display based nano immunosensors for cholera toxin Li, Yang Yang, Kai-di Kong, De-cai Ye, Jun-feng Front Immunol Immunology Cholera, a persistent global public health concern, continues to cause outbreaks in approximately 30 countries and territories this year. The imperative to safeguard water sources and food from Vibrio cholerae, the causative pathogen, remains urgent. The bacterium is mainly disseminated via ingestion of contaminated water or food. Despite the plate method’s gold standard status for detection, its time-consuming nature, taking several days to provide results, remains a challenge. The emergence of novel virulence serotypes raises public health concerns, potentially compromising existing detection methods. Hence, exploiting Vibrio cholerae toxin testing holds promise due to its inherent stability. Immunobiosensors, leveraging antibody specificity and sensitivity, present formidable tools for detecting diverse small molecules, encompassing drugs, hormones, toxins, and environmental pollutants. This review explores cholera toxin detection, highlighting phage display-based nano immunosensors’ potential. Engineered bacteriophages exhibit exceptional cholera toxin affinity, through specific antibody fragments or mimotopes, enabling precise quantification. This innovative approach promises to reshape cholera toxin detection, offering an alternative to animal-derived methods. Harnessing engineered bacteriophages aligns with ethical detection and emphasizes sensitivity and accuracy, a pivotal stride in the evolution of detection strategies. This review primarily introduces recent advancements in phage display-based nano immunosensors for cholera toxin, encompassing technical aspects, current challenges, and future prospects. Frontiers Media S.A. 2023-09-13 /pmc/articles/PMC10534012/ /pubmed/37781379 http://dx.doi.org/10.3389/fimmu.2023.1224397 Text en Copyright © 2023 Li, Yang, Kong and Ye https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Li, Yang
Yang, Kai-di
Kong, De-cai
Ye, Jun-feng
Advances in phage display based nano immunosensors for cholera toxin
title Advances in phage display based nano immunosensors for cholera toxin
title_full Advances in phage display based nano immunosensors for cholera toxin
title_fullStr Advances in phage display based nano immunosensors for cholera toxin
title_full_unstemmed Advances in phage display based nano immunosensors for cholera toxin
title_short Advances in phage display based nano immunosensors for cholera toxin
title_sort advances in phage display based nano immunosensors for cholera toxin
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10534012/
https://www.ncbi.nlm.nih.gov/pubmed/37781379
http://dx.doi.org/10.3389/fimmu.2023.1224397
work_keys_str_mv AT liyang advancesinphagedisplaybasednanoimmunosensorsforcholeratoxin
AT yangkaidi advancesinphagedisplaybasednanoimmunosensorsforcholeratoxin
AT kongdecai advancesinphagedisplaybasednanoimmunosensorsforcholeratoxin
AT yejunfeng advancesinphagedisplaybasednanoimmunosensorsforcholeratoxin