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Aqueous two-phase systems coupled with chemometrics-enhanced HPLC-DAD for simultaneous extraction and determination of flavonoids in honey
In this study, an accurate, rapid, green, and environment friendly method for the extraction and quantitative analysis of flavonoids in honey was established by using the aqueous two-phase extraction combined with the chemometrics-assisted HPLC-DAD. The first purpose of this study was to extract sev...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10534099/ https://www.ncbi.nlm.nih.gov/pubmed/37780266 http://dx.doi.org/10.1016/j.fochx.2023.100766 |
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author | Zhang, Xiao-Hua Qing, Xiang-Dong Zheng, Jing-Jing Yu, Yan Huang, Jiaojiao Kang, Chao Liu, Zhi |
author_facet | Zhang, Xiao-Hua Qing, Xiang-Dong Zheng, Jing-Jing Yu, Yan Huang, Jiaojiao Kang, Chao Liu, Zhi |
author_sort | Zhang, Xiao-Hua |
collection | PubMed |
description | In this study, an accurate, rapid, green, and environment friendly method for the extraction and quantitative analysis of flavonoids in honey was established by using the aqueous two-phase extraction combined with the chemometrics-assisted HPLC-DAD. The first purpose of this study was to extract seven flavonoids in five different types of honey using alcohol/salt aqueous two-phase system (ATPS). The system with 2.82 mL sodium citrate (30%), 1.58 mL water, and 3.10 mL isopropanol, showed the highest flavonoids extraction yields in the top phase (87.66–101.50%). Additionally, the three-way array of honey samples based on HPLC-DAD was decomposed mathematically by the alternating trilinear decomposition (ATLD) algorithm to obtain reasonable chromatograms, spectra, and concentration profiles for each analyte. Compared with the traditional solid-phase extraction method, the ATPS-ATLD-based method showed satisfactory spiked recoveries, lower limit of detection, and higher sensitivity, further verifying its accuracy and stability. |
format | Online Article Text |
id | pubmed-10534099 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-105340992023-09-29 Aqueous two-phase systems coupled with chemometrics-enhanced HPLC-DAD for simultaneous extraction and determination of flavonoids in honey Zhang, Xiao-Hua Qing, Xiang-Dong Zheng, Jing-Jing Yu, Yan Huang, Jiaojiao Kang, Chao Liu, Zhi Food Chem X Analytical Methods In this study, an accurate, rapid, green, and environment friendly method for the extraction and quantitative analysis of flavonoids in honey was established by using the aqueous two-phase extraction combined with the chemometrics-assisted HPLC-DAD. The first purpose of this study was to extract seven flavonoids in five different types of honey using alcohol/salt aqueous two-phase system (ATPS). The system with 2.82 mL sodium citrate (30%), 1.58 mL water, and 3.10 mL isopropanol, showed the highest flavonoids extraction yields in the top phase (87.66–101.50%). Additionally, the three-way array of honey samples based on HPLC-DAD was decomposed mathematically by the alternating trilinear decomposition (ATLD) algorithm to obtain reasonable chromatograms, spectra, and concentration profiles for each analyte. Compared with the traditional solid-phase extraction method, the ATPS-ATLD-based method showed satisfactory spiked recoveries, lower limit of detection, and higher sensitivity, further verifying its accuracy and stability. Elsevier 2023-06-21 /pmc/articles/PMC10534099/ /pubmed/37780266 http://dx.doi.org/10.1016/j.fochx.2023.100766 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Analytical Methods Zhang, Xiao-Hua Qing, Xiang-Dong Zheng, Jing-Jing Yu, Yan Huang, Jiaojiao Kang, Chao Liu, Zhi Aqueous two-phase systems coupled with chemometrics-enhanced HPLC-DAD for simultaneous extraction and determination of flavonoids in honey |
title | Aqueous two-phase systems coupled with chemometrics-enhanced HPLC-DAD for simultaneous extraction and determination of flavonoids in honey |
title_full | Aqueous two-phase systems coupled with chemometrics-enhanced HPLC-DAD for simultaneous extraction and determination of flavonoids in honey |
title_fullStr | Aqueous two-phase systems coupled with chemometrics-enhanced HPLC-DAD for simultaneous extraction and determination of flavonoids in honey |
title_full_unstemmed | Aqueous two-phase systems coupled with chemometrics-enhanced HPLC-DAD for simultaneous extraction and determination of flavonoids in honey |
title_short | Aqueous two-phase systems coupled with chemometrics-enhanced HPLC-DAD for simultaneous extraction and determination of flavonoids in honey |
title_sort | aqueous two-phase systems coupled with chemometrics-enhanced hplc-dad for simultaneous extraction and determination of flavonoids in honey |
topic | Analytical Methods |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10534099/ https://www.ncbi.nlm.nih.gov/pubmed/37780266 http://dx.doi.org/10.1016/j.fochx.2023.100766 |
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