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Rapid detection of Salmonella in food matrices by photonic PCR based on the photothermal effect of Fe(3)O(4)
Salmonella causes most deaths from diarrheal disease worldwide. Therefore, Salmonella must be accurately and quickly detected, even in complex food matrices, which is difficult to achieve using conventional culture methods. Here we propose a novel photonic polymerase chain reaction (PCR) method base...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10534150/ https://www.ncbi.nlm.nih.gov/pubmed/37780326 http://dx.doi.org/10.1016/j.fochx.2023.100798 |
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author | Jiao, Yuru Zhang, Zhen Wang, Kaifei Zhang, Hongyan Gao, Jianxin |
author_facet | Jiao, Yuru Zhang, Zhen Wang, Kaifei Zhang, Hongyan Gao, Jianxin |
author_sort | Jiao, Yuru |
collection | PubMed |
description | Salmonella causes most deaths from diarrheal disease worldwide. Therefore, Salmonella must be accurately and quickly detected, even in complex food matrices, which is difficult to achieve using conventional culture methods. Here we propose a novel photonic polymerase chain reaction (PCR) method based on ferroferric oxide (Fe(3)O(4)) for the detection of Salmonella typhimurium in complex samples. Owing to the great photothermal conversion performance of Fe(3)O(4), rapid thermal cycling could be accomplished. Our optimized photonic PCR system specifically detected Salmonella typhimurium in complex food matrices within 50 min. Quantitative data showed a limit of detection up to 10(2) CFU/mL in food samples. This method is suitable for the detection of all pathogenic microorganisms and is universal. |
format | Online Article Text |
id | pubmed-10534150 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-105341502023-09-29 Rapid detection of Salmonella in food matrices by photonic PCR based on the photothermal effect of Fe(3)O(4) Jiao, Yuru Zhang, Zhen Wang, Kaifei Zhang, Hongyan Gao, Jianxin Food Chem X Research Article Salmonella causes most deaths from diarrheal disease worldwide. Therefore, Salmonella must be accurately and quickly detected, even in complex food matrices, which is difficult to achieve using conventional culture methods. Here we propose a novel photonic polymerase chain reaction (PCR) method based on ferroferric oxide (Fe(3)O(4)) for the detection of Salmonella typhimurium in complex samples. Owing to the great photothermal conversion performance of Fe(3)O(4), rapid thermal cycling could be accomplished. Our optimized photonic PCR system specifically detected Salmonella typhimurium in complex food matrices within 50 min. Quantitative data showed a limit of detection up to 10(2) CFU/mL in food samples. This method is suitable for the detection of all pathogenic microorganisms and is universal. Elsevier 2023-07-19 /pmc/articles/PMC10534150/ /pubmed/37780326 http://dx.doi.org/10.1016/j.fochx.2023.100798 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Jiao, Yuru Zhang, Zhen Wang, Kaifei Zhang, Hongyan Gao, Jianxin Rapid detection of Salmonella in food matrices by photonic PCR based on the photothermal effect of Fe(3)O(4) |
title | Rapid detection of Salmonella in food matrices by photonic PCR based on the photothermal effect of Fe(3)O(4) |
title_full | Rapid detection of Salmonella in food matrices by photonic PCR based on the photothermal effect of Fe(3)O(4) |
title_fullStr | Rapid detection of Salmonella in food matrices by photonic PCR based on the photothermal effect of Fe(3)O(4) |
title_full_unstemmed | Rapid detection of Salmonella in food matrices by photonic PCR based on the photothermal effect of Fe(3)O(4) |
title_short | Rapid detection of Salmonella in food matrices by photonic PCR based on the photothermal effect of Fe(3)O(4) |
title_sort | rapid detection of salmonella in food matrices by photonic pcr based on the photothermal effect of fe(3)o(4) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10534150/ https://www.ncbi.nlm.nih.gov/pubmed/37780326 http://dx.doi.org/10.1016/j.fochx.2023.100798 |
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