The Separation, Purification, Structure Identification, and Antioxidant Activity of Elaeagnus umbellata Polysaccharides

In order to investigate the antioxidant activity of Elaeagnus umbellata polysaccharides, the physicochemical characteristics of purified Elaeagnus umbellata polysaccharides (EUP, consisting of two fractions, EUP1 and EUP2) were investigated using UV spectrophotometry, high-performance liquid chromat...

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Autores principales: Zhang, Jinhua, Xu, Xin, Liu, Xinyi, Chen, Min, Bai, Baoqing, Yang, Yukun, Bo, Tao, Fan, Sanhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10534330/
https://www.ncbi.nlm.nih.gov/pubmed/37764243
http://dx.doi.org/10.3390/molecules28186468
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author Zhang, Jinhua
Xu, Xin
Liu, Xinyi
Chen, Min
Bai, Baoqing
Yang, Yukun
Bo, Tao
Fan, Sanhong
author_facet Zhang, Jinhua
Xu, Xin
Liu, Xinyi
Chen, Min
Bai, Baoqing
Yang, Yukun
Bo, Tao
Fan, Sanhong
author_sort Zhang, Jinhua
collection PubMed
description In order to investigate the antioxidant activity of Elaeagnus umbellata polysaccharides, the physicochemical characteristics of purified Elaeagnus umbellata polysaccharides (EUP, consisting of two fractions, EUP1 and EUP2) were investigated using UV spectrophotometry, high-performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), and Fourier transform infrared spectroscopy (FT-IR). This revealed that EUP1 and EUP2 were acidic polysaccharides with an average molecular weight (MW) of 63 and 38 kDa, respectively. EUP1 mainly consisted of L-rhamnose and D-galactose in a molar ratio of 2.05:1, and EUP2 consisted of D-mannose, L-rhamnose, D-galactose, and D-arabinose in a molar ratio of 2.06:1:2.78:1. Furthermore, EUP exhibited considerable antioxidant potential for scavenging hydroxyl, superoxide anion, DPPH, and ABTS radicals. Therefore, EUP can be developed as a potential antioxidant for the functional food or pharmaceutical field.
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spelling pubmed-105343302023-09-29 The Separation, Purification, Structure Identification, and Antioxidant Activity of Elaeagnus umbellata Polysaccharides Zhang, Jinhua Xu, Xin Liu, Xinyi Chen, Min Bai, Baoqing Yang, Yukun Bo, Tao Fan, Sanhong Molecules Article In order to investigate the antioxidant activity of Elaeagnus umbellata polysaccharides, the physicochemical characteristics of purified Elaeagnus umbellata polysaccharides (EUP, consisting of two fractions, EUP1 and EUP2) were investigated using UV spectrophotometry, high-performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), and Fourier transform infrared spectroscopy (FT-IR). This revealed that EUP1 and EUP2 were acidic polysaccharides with an average molecular weight (MW) of 63 and 38 kDa, respectively. EUP1 mainly consisted of L-rhamnose and D-galactose in a molar ratio of 2.05:1, and EUP2 consisted of D-mannose, L-rhamnose, D-galactose, and D-arabinose in a molar ratio of 2.06:1:2.78:1. Furthermore, EUP exhibited considerable antioxidant potential for scavenging hydroxyl, superoxide anion, DPPH, and ABTS radicals. Therefore, EUP can be developed as a potential antioxidant for the functional food or pharmaceutical field. MDPI 2023-09-06 /pmc/articles/PMC10534330/ /pubmed/37764243 http://dx.doi.org/10.3390/molecules28186468 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Jinhua
Xu, Xin
Liu, Xinyi
Chen, Min
Bai, Baoqing
Yang, Yukun
Bo, Tao
Fan, Sanhong
The Separation, Purification, Structure Identification, and Antioxidant Activity of Elaeagnus umbellata Polysaccharides
title The Separation, Purification, Structure Identification, and Antioxidant Activity of Elaeagnus umbellata Polysaccharides
title_full The Separation, Purification, Structure Identification, and Antioxidant Activity of Elaeagnus umbellata Polysaccharides
title_fullStr The Separation, Purification, Structure Identification, and Antioxidant Activity of Elaeagnus umbellata Polysaccharides
title_full_unstemmed The Separation, Purification, Structure Identification, and Antioxidant Activity of Elaeagnus umbellata Polysaccharides
title_short The Separation, Purification, Structure Identification, and Antioxidant Activity of Elaeagnus umbellata Polysaccharides
title_sort separation, purification, structure identification, and antioxidant activity of elaeagnus umbellata polysaccharides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10534330/
https://www.ncbi.nlm.nih.gov/pubmed/37764243
http://dx.doi.org/10.3390/molecules28186468
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