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A Multilayered Imaging and Microfluidics Approach for Evaluating the Effect of Fibrinolysis in Staphylococcus aureus Biofilm Formation

The recognition of microbe and extracellular matrix (ECM) is a recurring theme in the humoral innate immune system. Fluid-phase molecules of innate immunity share regulatory roles in ECM. On the other hand, ECM elements have immunological functions. Innate immunity is evolutionary and functionally c...

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Autores principales: Parente, Raffaella, Fumagalli, Maria Rita, Di Claudio, Alessia, Cárdenas Rincón, Cindy Lorena, Erreni, Marco, Zanini, Damiano, Iapichino, Giacomo, Protti, Alessandro, Garlanda, Cecilia, Rusconi, Roberto, Doni, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10534389/
https://www.ncbi.nlm.nih.gov/pubmed/37764949
http://dx.doi.org/10.3390/pathogens12091141
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author Parente, Raffaella
Fumagalli, Maria Rita
Di Claudio, Alessia
Cárdenas Rincón, Cindy Lorena
Erreni, Marco
Zanini, Damiano
Iapichino, Giacomo
Protti, Alessandro
Garlanda, Cecilia
Rusconi, Roberto
Doni, Andrea
author_facet Parente, Raffaella
Fumagalli, Maria Rita
Di Claudio, Alessia
Cárdenas Rincón, Cindy Lorena
Erreni, Marco
Zanini, Damiano
Iapichino, Giacomo
Protti, Alessandro
Garlanda, Cecilia
Rusconi, Roberto
Doni, Andrea
author_sort Parente, Raffaella
collection PubMed
description The recognition of microbe and extracellular matrix (ECM) is a recurring theme in the humoral innate immune system. Fluid-phase molecules of innate immunity share regulatory roles in ECM. On the other hand, ECM elements have immunological functions. Innate immunity is evolutionary and functionally connected to hemostasis. Staphylococcus aureus (S. aureus) is a major cause of hospital-associated bloodstream infections and the most common cause of several life-threatening conditions such as endocarditis and sepsis through its ability to manipulate hemostasis. Biofilm-related infection and sepsis represent a medical need due to the lack of treatments and the high resistance to antibiotics. We designed a method combining imaging and microfluidics to dissect the role of elements of the ECM and hemostasis in triggering S. aureus biofilm by highlighting an essential role of fibrinogen (FG) in adhesion and formation. Furthermore, we ascertained an important role of the fluid-phase activation of fibrinolysis in inhibiting biofilm of S. aureus and facilitating an antibody-mediated response aimed at pathogen killing. The results define FG as an essential element of hemostasis in the S. aureus biofilm formation and a role of fibrinolysis in its inhibition, while promoting an antibody-mediated response. Understanding host molecular mechanisms influencing biofilm formation and degradation is instrumental for the development of new combined therapeutic approaches to prevent the risk of S. aureus biofilm-associated diseases.
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spelling pubmed-105343892023-09-29 A Multilayered Imaging and Microfluidics Approach for Evaluating the Effect of Fibrinolysis in Staphylococcus aureus Biofilm Formation Parente, Raffaella Fumagalli, Maria Rita Di Claudio, Alessia Cárdenas Rincón, Cindy Lorena Erreni, Marco Zanini, Damiano Iapichino, Giacomo Protti, Alessandro Garlanda, Cecilia Rusconi, Roberto Doni, Andrea Pathogens Article The recognition of microbe and extracellular matrix (ECM) is a recurring theme in the humoral innate immune system. Fluid-phase molecules of innate immunity share regulatory roles in ECM. On the other hand, ECM elements have immunological functions. Innate immunity is evolutionary and functionally connected to hemostasis. Staphylococcus aureus (S. aureus) is a major cause of hospital-associated bloodstream infections and the most common cause of several life-threatening conditions such as endocarditis and sepsis through its ability to manipulate hemostasis. Biofilm-related infection and sepsis represent a medical need due to the lack of treatments and the high resistance to antibiotics. We designed a method combining imaging and microfluidics to dissect the role of elements of the ECM and hemostasis in triggering S. aureus biofilm by highlighting an essential role of fibrinogen (FG) in adhesion and formation. Furthermore, we ascertained an important role of the fluid-phase activation of fibrinolysis in inhibiting biofilm of S. aureus and facilitating an antibody-mediated response aimed at pathogen killing. The results define FG as an essential element of hemostasis in the S. aureus biofilm formation and a role of fibrinolysis in its inhibition, while promoting an antibody-mediated response. Understanding host molecular mechanisms influencing biofilm formation and degradation is instrumental for the development of new combined therapeutic approaches to prevent the risk of S. aureus biofilm-associated diseases. MDPI 2023-09-06 /pmc/articles/PMC10534389/ /pubmed/37764949 http://dx.doi.org/10.3390/pathogens12091141 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Parente, Raffaella
Fumagalli, Maria Rita
Di Claudio, Alessia
Cárdenas Rincón, Cindy Lorena
Erreni, Marco
Zanini, Damiano
Iapichino, Giacomo
Protti, Alessandro
Garlanda, Cecilia
Rusconi, Roberto
Doni, Andrea
A Multilayered Imaging and Microfluidics Approach for Evaluating the Effect of Fibrinolysis in Staphylococcus aureus Biofilm Formation
title A Multilayered Imaging and Microfluidics Approach for Evaluating the Effect of Fibrinolysis in Staphylococcus aureus Biofilm Formation
title_full A Multilayered Imaging and Microfluidics Approach for Evaluating the Effect of Fibrinolysis in Staphylococcus aureus Biofilm Formation
title_fullStr A Multilayered Imaging and Microfluidics Approach for Evaluating the Effect of Fibrinolysis in Staphylococcus aureus Biofilm Formation
title_full_unstemmed A Multilayered Imaging and Microfluidics Approach for Evaluating the Effect of Fibrinolysis in Staphylococcus aureus Biofilm Formation
title_short A Multilayered Imaging and Microfluidics Approach for Evaluating the Effect of Fibrinolysis in Staphylococcus aureus Biofilm Formation
title_sort multilayered imaging and microfluidics approach for evaluating the effect of fibrinolysis in staphylococcus aureus biofilm formation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10534389/
https://www.ncbi.nlm.nih.gov/pubmed/37764949
http://dx.doi.org/10.3390/pathogens12091141
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