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Fluorescence Tracking of Small Extracellular Vesicles In Vivo
In this study, we employed organic and inorganic dyes that have fluorescence under visible or near-infrared light region to stain human umbilical cord (Huc) mesenchymal stem cell (MSC)-, HEK293T cell- and HGC cell-derived small extracellular vesicles (sEVs), and then tracked their fluorescence signa...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10534450/ https://www.ncbi.nlm.nih.gov/pubmed/37765266 http://dx.doi.org/10.3390/pharmaceutics15092297 |
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author | Chen, Yanxia Shi, Yinghong Tao, Zhimin |
author_facet | Chen, Yanxia Shi, Yinghong Tao, Zhimin |
author_sort | Chen, Yanxia |
collection | PubMed |
description | In this study, we employed organic and inorganic dyes that have fluorescence under visible or near-infrared light region to stain human umbilical cord (Huc) mesenchymal stem cell (MSC)-, HEK293T cell- and HGC cell-derived small extracellular vesicles (sEVs), and then tracked their fluorescence signals in human gastric cancer xenografted murine models. Several biological characteristics were examined and compared when different dye-stained sEVs in the same tumor model or the same dye-stained sEVs between different tumor models were applied, including sEVs circulation in the blood, biodistribution of sEVs in major organs, and time-dependent tumor accumulation of sEVs. The results demonstrated that distinct tumor accumulation features were presented by sEVs if labeled by different fluorescent dyes, while sEVs derived from different cell lines showed homologous blood circulation and tumor accumulation. To conclude, although fluorescence imaging remains a reliable way to trace sEVs, single staining of sEVs membrane should be obviated in future work when examining the biological fate of sEVs. |
format | Online Article Text |
id | pubmed-10534450 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105344502023-09-29 Fluorescence Tracking of Small Extracellular Vesicles In Vivo Chen, Yanxia Shi, Yinghong Tao, Zhimin Pharmaceutics Article In this study, we employed organic and inorganic dyes that have fluorescence under visible or near-infrared light region to stain human umbilical cord (Huc) mesenchymal stem cell (MSC)-, HEK293T cell- and HGC cell-derived small extracellular vesicles (sEVs), and then tracked their fluorescence signals in human gastric cancer xenografted murine models. Several biological characteristics were examined and compared when different dye-stained sEVs in the same tumor model or the same dye-stained sEVs between different tumor models were applied, including sEVs circulation in the blood, biodistribution of sEVs in major organs, and time-dependent tumor accumulation of sEVs. The results demonstrated that distinct tumor accumulation features were presented by sEVs if labeled by different fluorescent dyes, while sEVs derived from different cell lines showed homologous blood circulation and tumor accumulation. To conclude, although fluorescence imaging remains a reliable way to trace sEVs, single staining of sEVs membrane should be obviated in future work when examining the biological fate of sEVs. MDPI 2023-09-08 /pmc/articles/PMC10534450/ /pubmed/37765266 http://dx.doi.org/10.3390/pharmaceutics15092297 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chen, Yanxia Shi, Yinghong Tao, Zhimin Fluorescence Tracking of Small Extracellular Vesicles In Vivo |
title | Fluorescence Tracking of Small Extracellular Vesicles In Vivo |
title_full | Fluorescence Tracking of Small Extracellular Vesicles In Vivo |
title_fullStr | Fluorescence Tracking of Small Extracellular Vesicles In Vivo |
title_full_unstemmed | Fluorescence Tracking of Small Extracellular Vesicles In Vivo |
title_short | Fluorescence Tracking of Small Extracellular Vesicles In Vivo |
title_sort | fluorescence tracking of small extracellular vesicles in vivo |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10534450/ https://www.ncbi.nlm.nih.gov/pubmed/37765266 http://dx.doi.org/10.3390/pharmaceutics15092297 |
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