Cargando…
Advances in Biosynthesis of Non-Canonical Amino Acids (ncAAs) and the Methods of ncAAs Incorporation into Proteins
The functional pool of canonical amino acids (cAAs) has been enriched through the emergence of non-canonical amino acids (ncAAs). NcAAs play a crucial role in the production of various pharmaceuticals. The biosynthesis of ncAAs has emerged as an alternative to traditional chemical synthesis due to i...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10534643/ https://www.ncbi.nlm.nih.gov/pubmed/37764520 http://dx.doi.org/10.3390/molecules28186745 |
_version_ | 1785112442813022208 |
---|---|
author | Chen, Liang Xin, Xiulan Zhang, Yuning Li, Shunyao Zhao, Xiaoman Li, Song Xu, Zhaochu |
author_facet | Chen, Liang Xin, Xiulan Zhang, Yuning Li, Shunyao Zhao, Xiaoman Li, Song Xu, Zhaochu |
author_sort | Chen, Liang |
collection | PubMed |
description | The functional pool of canonical amino acids (cAAs) has been enriched through the emergence of non-canonical amino acids (ncAAs). NcAAs play a crucial role in the production of various pharmaceuticals. The biosynthesis of ncAAs has emerged as an alternative to traditional chemical synthesis due to its environmental friendliness and high efficiency. The breakthrough genetic code expansion (GCE) technique developed in recent years has allowed the incorporation of ncAAs into target proteins, giving them special functions and biological activities. The biosynthesis of ncAAs and their incorporation into target proteins within a single microbe has become an enticing application of such molecules. Based on that, in this study, we first review the biosynthesis methods for ncAAs and analyze the difficulties related to biosynthesis. We then summarize the GCE methods and analyze their advantages and disadvantages. Further, we review the application progress of ncAAs and anticipate the challenges and future development directions of ncAAs. |
format | Online Article Text |
id | pubmed-10534643 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105346432023-09-29 Advances in Biosynthesis of Non-Canonical Amino Acids (ncAAs) and the Methods of ncAAs Incorporation into Proteins Chen, Liang Xin, Xiulan Zhang, Yuning Li, Shunyao Zhao, Xiaoman Li, Song Xu, Zhaochu Molecules Review The functional pool of canonical amino acids (cAAs) has been enriched through the emergence of non-canonical amino acids (ncAAs). NcAAs play a crucial role in the production of various pharmaceuticals. The biosynthesis of ncAAs has emerged as an alternative to traditional chemical synthesis due to its environmental friendliness and high efficiency. The breakthrough genetic code expansion (GCE) technique developed in recent years has allowed the incorporation of ncAAs into target proteins, giving them special functions and biological activities. The biosynthesis of ncAAs and their incorporation into target proteins within a single microbe has become an enticing application of such molecules. Based on that, in this study, we first review the biosynthesis methods for ncAAs and analyze the difficulties related to biosynthesis. We then summarize the GCE methods and analyze their advantages and disadvantages. Further, we review the application progress of ncAAs and anticipate the challenges and future development directions of ncAAs. MDPI 2023-09-21 /pmc/articles/PMC10534643/ /pubmed/37764520 http://dx.doi.org/10.3390/molecules28186745 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Chen, Liang Xin, Xiulan Zhang, Yuning Li, Shunyao Zhao, Xiaoman Li, Song Xu, Zhaochu Advances in Biosynthesis of Non-Canonical Amino Acids (ncAAs) and the Methods of ncAAs Incorporation into Proteins |
title | Advances in Biosynthesis of Non-Canonical Amino Acids (ncAAs) and the Methods of ncAAs Incorporation into Proteins |
title_full | Advances in Biosynthesis of Non-Canonical Amino Acids (ncAAs) and the Methods of ncAAs Incorporation into Proteins |
title_fullStr | Advances in Biosynthesis of Non-Canonical Amino Acids (ncAAs) and the Methods of ncAAs Incorporation into Proteins |
title_full_unstemmed | Advances in Biosynthesis of Non-Canonical Amino Acids (ncAAs) and the Methods of ncAAs Incorporation into Proteins |
title_short | Advances in Biosynthesis of Non-Canonical Amino Acids (ncAAs) and the Methods of ncAAs Incorporation into Proteins |
title_sort | advances in biosynthesis of non-canonical amino acids (ncaas) and the methods of ncaas incorporation into proteins |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10534643/ https://www.ncbi.nlm.nih.gov/pubmed/37764520 http://dx.doi.org/10.3390/molecules28186745 |
work_keys_str_mv | AT chenliang advancesinbiosynthesisofnoncanonicalaminoacidsncaasandthemethodsofncaasincorporationintoproteins AT xinxiulan advancesinbiosynthesisofnoncanonicalaminoacidsncaasandthemethodsofncaasincorporationintoproteins AT zhangyuning advancesinbiosynthesisofnoncanonicalaminoacidsncaasandthemethodsofncaasincorporationintoproteins AT lishunyao advancesinbiosynthesisofnoncanonicalaminoacidsncaasandthemethodsofncaasincorporationintoproteins AT zhaoxiaoman advancesinbiosynthesisofnoncanonicalaminoacidsncaasandthemethodsofncaasincorporationintoproteins AT lisong advancesinbiosynthesisofnoncanonicalaminoacidsncaasandthemethodsofncaasincorporationintoproteins AT xuzhaochu advancesinbiosynthesisofnoncanonicalaminoacidsncaasandthemethodsofncaasincorporationintoproteins |