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A Multiplex Quantitative Polymerase Chain Reaction for the Rapid Differential Detection of Subgroups A, B, J, and K Avian Leukosis Viruses

Avian leukosis (AL), caused by avian leukosis virus (ALV), is a contagious tumor disease that results in significant economic losses for the poultry industry. Currently, ALV-A, B, J, and K subgroups are the most common in commercial poultry and cause possible coinfections. Therefore, close monitorin...

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Autores principales: Dou, Junfeng, Wang, Zui, Li, Li, Lu, Qin, Jin, Xinxin, Ling, Xiaochun, Cheng, Zhengyu, Zhang, Tengfei, Shao, Huabin, Zhai, Xinguo, Luo, Qingping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10535029/
https://www.ncbi.nlm.nih.gov/pubmed/37766196
http://dx.doi.org/10.3390/v15091789
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author Dou, Junfeng
Wang, Zui
Li, Li
Lu, Qin
Jin, Xinxin
Ling, Xiaochun
Cheng, Zhengyu
Zhang, Tengfei
Shao, Huabin
Zhai, Xinguo
Luo, Qingping
author_facet Dou, Junfeng
Wang, Zui
Li, Li
Lu, Qin
Jin, Xinxin
Ling, Xiaochun
Cheng, Zhengyu
Zhang, Tengfei
Shao, Huabin
Zhai, Xinguo
Luo, Qingping
author_sort Dou, Junfeng
collection PubMed
description Avian leukosis (AL), caused by avian leukosis virus (ALV), is a contagious tumor disease that results in significant economic losses for the poultry industry. Currently, ALV-A, B, J, and K subgroups are the most common in commercial poultry and cause possible coinfections. Therefore, close monitoring is necessary to avoid greater economic losses. In this study, a novel multiplex quantitative polymerase chain reaction (qPCR) assay was developed to detect ALV-A, ALV-B, ALV-J, and ALV-K with limits of detection of 40, 11, 13.7, and 96 copies/µL, respectively, and no cross-reactivity with other ALV subtypes and avian pathogens. We detected 852 cell cultures inoculated with clinical samples using this method, showing good consistency with conventional PCR and ELISA. The most prevalent ALV strain in Hubei Province, China, was still ALV-J (11.74%). Although single infections with ALV-A, ALV-B, and ALV-K were not found, coinfections with different subgroup strains were identified: 0.7% for ALV-A/J, 0.35% for ALV-B/J, 0.25% for ALV-J/K, and 0.12% for ALV-A/B/K and ALV-A/B/J. Therefore, our novel multiplex qPCR may be a useful tool for molecular epidemiology, clinical detection of ALV, and ALV eradication programs.
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spelling pubmed-105350292023-09-29 A Multiplex Quantitative Polymerase Chain Reaction for the Rapid Differential Detection of Subgroups A, B, J, and K Avian Leukosis Viruses Dou, Junfeng Wang, Zui Li, Li Lu, Qin Jin, Xinxin Ling, Xiaochun Cheng, Zhengyu Zhang, Tengfei Shao, Huabin Zhai, Xinguo Luo, Qingping Viruses Article Avian leukosis (AL), caused by avian leukosis virus (ALV), is a contagious tumor disease that results in significant economic losses for the poultry industry. Currently, ALV-A, B, J, and K subgroups are the most common in commercial poultry and cause possible coinfections. Therefore, close monitoring is necessary to avoid greater economic losses. In this study, a novel multiplex quantitative polymerase chain reaction (qPCR) assay was developed to detect ALV-A, ALV-B, ALV-J, and ALV-K with limits of detection of 40, 11, 13.7, and 96 copies/µL, respectively, and no cross-reactivity with other ALV subtypes and avian pathogens. We detected 852 cell cultures inoculated with clinical samples using this method, showing good consistency with conventional PCR and ELISA. The most prevalent ALV strain in Hubei Province, China, was still ALV-J (11.74%). Although single infections with ALV-A, ALV-B, and ALV-K were not found, coinfections with different subgroup strains were identified: 0.7% for ALV-A/J, 0.35% for ALV-B/J, 0.25% for ALV-J/K, and 0.12% for ALV-A/B/K and ALV-A/B/J. Therefore, our novel multiplex qPCR may be a useful tool for molecular epidemiology, clinical detection of ALV, and ALV eradication programs. MDPI 2023-08-23 /pmc/articles/PMC10535029/ /pubmed/37766196 http://dx.doi.org/10.3390/v15091789 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dou, Junfeng
Wang, Zui
Li, Li
Lu, Qin
Jin, Xinxin
Ling, Xiaochun
Cheng, Zhengyu
Zhang, Tengfei
Shao, Huabin
Zhai, Xinguo
Luo, Qingping
A Multiplex Quantitative Polymerase Chain Reaction for the Rapid Differential Detection of Subgroups A, B, J, and K Avian Leukosis Viruses
title A Multiplex Quantitative Polymerase Chain Reaction for the Rapid Differential Detection of Subgroups A, B, J, and K Avian Leukosis Viruses
title_full A Multiplex Quantitative Polymerase Chain Reaction for the Rapid Differential Detection of Subgroups A, B, J, and K Avian Leukosis Viruses
title_fullStr A Multiplex Quantitative Polymerase Chain Reaction for the Rapid Differential Detection of Subgroups A, B, J, and K Avian Leukosis Viruses
title_full_unstemmed A Multiplex Quantitative Polymerase Chain Reaction for the Rapid Differential Detection of Subgroups A, B, J, and K Avian Leukosis Viruses
title_short A Multiplex Quantitative Polymerase Chain Reaction for the Rapid Differential Detection of Subgroups A, B, J, and K Avian Leukosis Viruses
title_sort multiplex quantitative polymerase chain reaction for the rapid differential detection of subgroups a, b, j, and k avian leukosis viruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10535029/
https://www.ncbi.nlm.nih.gov/pubmed/37766196
http://dx.doi.org/10.3390/v15091789
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