Electrocatalytic Determination of Uric Acid with the Poly(Tartrazine)-Modified Pencil Graphite Electrode in Human Serum and Artificial Urine

[Image: see text] A novel electrocatalytic sensing strategy was built for uric acid (UA) determination with an exceptionally developed poly(tartrazine)-modified activated pencil graphite electrode (pTRT/aPGE) in human serum and artificial urine. The oxidation signal of UA at 275 mV in pH 7.5 phospha...

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Autores principales: Liv, Lokman, Portakal, Merve, Çukur, Meryem Sıla, Topaçlı, Beyza, Uzun, Berkay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10535258/
https://www.ncbi.nlm.nih.gov/pubmed/37780010
http://dx.doi.org/10.1021/acsomega.3c02561
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author Liv, Lokman
Portakal, Merve
Çukur, Meryem Sıla
Topaçlı, Beyza
Uzun, Berkay
author_facet Liv, Lokman
Portakal, Merve
Çukur, Meryem Sıla
Topaçlı, Beyza
Uzun, Berkay
author_sort Liv, Lokman
collection PubMed
description [Image: see text] A novel electrocatalytic sensing strategy was built for uric acid (UA) determination with an exceptionally developed poly(tartrazine)-modified activated pencil graphite electrode (pTRT/aPGE) in human serum and artificial urine. The oxidation signal of UA at 275 mV in pH 7.5 phosphate buffer solution served as the analytical response. Cyclic voltammetry, electrochemical impedance spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray photoelectron spectroscopy were used to characterize the sensing platform, which was able to detect 0.10 μM of UA in the ranges of 0.34–60 and 70–140 μM. The samples of human serum and artificial urine were analyzed by both the pTRT/aPGE and the uricase-modified screen-printed electrode. The results were statistically evaluated and compared with each other within the confidence level of 95%, and no significant difference between the results was found.
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spelling pubmed-105352582023-09-29 Electrocatalytic Determination of Uric Acid with the Poly(Tartrazine)-Modified Pencil Graphite Electrode in Human Serum and Artificial Urine Liv, Lokman Portakal, Merve Çukur, Meryem Sıla Topaçlı, Beyza Uzun, Berkay ACS Omega [Image: see text] A novel electrocatalytic sensing strategy was built for uric acid (UA) determination with an exceptionally developed poly(tartrazine)-modified activated pencil graphite electrode (pTRT/aPGE) in human serum and artificial urine. The oxidation signal of UA at 275 mV in pH 7.5 phosphate buffer solution served as the analytical response. Cyclic voltammetry, electrochemical impedance spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray photoelectron spectroscopy were used to characterize the sensing platform, which was able to detect 0.10 μM of UA in the ranges of 0.34–60 and 70–140 μM. The samples of human serum and artificial urine were analyzed by both the pTRT/aPGE and the uricase-modified screen-printed electrode. The results were statistically evaluated and compared with each other within the confidence level of 95%, and no significant difference between the results was found. American Chemical Society 2023-09-11 /pmc/articles/PMC10535258/ /pubmed/37780010 http://dx.doi.org/10.1021/acsomega.3c02561 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Liv, Lokman
Portakal, Merve
Çukur, Meryem Sıla
Topaçlı, Beyza
Uzun, Berkay
Electrocatalytic Determination of Uric Acid with the Poly(Tartrazine)-Modified Pencil Graphite Electrode in Human Serum and Artificial Urine
title Electrocatalytic Determination of Uric Acid with the Poly(Tartrazine)-Modified Pencil Graphite Electrode in Human Serum and Artificial Urine
title_full Electrocatalytic Determination of Uric Acid with the Poly(Tartrazine)-Modified Pencil Graphite Electrode in Human Serum and Artificial Urine
title_fullStr Electrocatalytic Determination of Uric Acid with the Poly(Tartrazine)-Modified Pencil Graphite Electrode in Human Serum and Artificial Urine
title_full_unstemmed Electrocatalytic Determination of Uric Acid with the Poly(Tartrazine)-Modified Pencil Graphite Electrode in Human Serum and Artificial Urine
title_short Electrocatalytic Determination of Uric Acid with the Poly(Tartrazine)-Modified Pencil Graphite Electrode in Human Serum and Artificial Urine
title_sort electrocatalytic determination of uric acid with the poly(tartrazine)-modified pencil graphite electrode in human serum and artificial urine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10535258/
https://www.ncbi.nlm.nih.gov/pubmed/37780010
http://dx.doi.org/10.1021/acsomega.3c02561
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